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Arcobacter contamination on pre- and post-chilled bovine carcasses and in minced beef at retail

Sarah De Smet UGent, Lieven De Zutter UGent, Johan Van Hende UGent and Kurt Houf UGent (2010) JOURNAL OF APPLIED MICROBIOLOGY. 108(1).
abstract
The present study aimed to assess the Arcobacter contamination on bovine carcasses postevisceration and postcooling in two slaughterhouses and in ready-to-eat minced beef. Methods and Results: Carcasses (n = 247) were sampled at four sites in two slaughterhouses and 100 minced beef samples were collected at retail. Isolation was performed by a quantitative and qualitative Arcobacter selective method, and the isolates were identified by multiplex PCR, after which a part of them were characterized by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Although arcobacters were isolated from 37% of the bovine carcasses postevisceration with the chest and the foreleg as most contaminated sites, cooling the carcasses for at least 24 h reduced the incidence of Arcobacter (7%) on the carcass surface significantly. Arcobacter butzleri was the species most frequently isolated, although co-contamination with multiple species also occurred. At retail, arcobacters were present in 9% of the minced beef samples, with Arcobacter butzleri as the dominant species. Conclusions: Forced air cooling of bovine carcasses for at least 24 h decreased the number of positive carcasses, but did not eliminate all arcobacters. Significance and Impact of the study: This study demonstrates that maintaining good hygiene practices throughout the food supply chain is crucial to ensure safe food products at the consumer level.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
AEROTOLERANT CAMPYLOBACTER, PATHOGEN ARCOBACTER, FECAL SAMPLES, HEALTHY CATTLE, POULTRY PRODUCTS, BUTZLERI, MEAT, PREVALENCE, SPP., PCR
journal title
JOURNAL OF APPLIED MICROBIOLOGY
J. Appl. Microbiol.
volume
108
issue
1
Web of Science type
article
Web of Science id
000272656800031
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.365 (2010)
JCR rank
64/158 (2010)
JCR quartile
2 (2010)
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2009.04430.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
998244
handle
http://hdl.handle.net/1854/LU-998244
date created
2010-06-30 13:59:37
date last changed
2010-07-02 11:33:05
@article{998244,
  abstract     = {The present study aimed to assess the Arcobacter contamination on bovine carcasses postevisceration and postcooling in two slaughterhouses and in ready-to-eat minced beef.
Methods and Results:
Carcasses (n = 247) were sampled at four sites in two slaughterhouses and 100 minced beef samples were collected at retail. Isolation was performed by a quantitative and qualitative Arcobacter selective method, and the isolates were identified by multiplex PCR, after which a part of them were characterized by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Although arcobacters were isolated from 37\% of the bovine carcasses postevisceration with the chest and the foreleg as most contaminated sites, cooling the carcasses for at least 24 h reduced the incidence of Arcobacter (7\%) on the carcass surface significantly. Arcobacter butzleri was the species most frequently isolated, although co-contamination with multiple species also occurred. At retail, arcobacters were present in 9\% of the minced beef samples, with Arcobacter butzleri as the dominant species.
Conclusions:
Forced air cooling of bovine carcasses for at least 24 h decreased the number of positive carcasses, but did not eliminate all arcobacters.
Significance and Impact of the study:
This study demonstrates that maintaining good hygiene practices throughout the food supply chain is crucial to ensure safe food products at the consumer level.},
  author       = {De Smet, Sarah and De Zutter, Lieven and Van Hende, Johan and Houf, Kurt},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keyword      = {AEROTOLERANT CAMPYLOBACTER,PATHOGEN ARCOBACTER,FECAL SAMPLES,HEALTHY CATTLE,POULTRY PRODUCTS,BUTZLERI,MEAT,PREVALENCE,SPP.,PCR},
  language     = {eng},
  number       = {1},
  title        = {Arcobacter contamination on pre- and post-chilled bovine carcasses and in minced beef at retail},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2009.04430.x},
  volume       = {108},
  year         = {2010},
}

Chicago
De Smet, Sarah, Lieven De Zutter, Johan Van Hende, and Kurt Houf. 2010. “Arcobacter Contamination on Pre- and Post-chilled Bovine Carcasses and in Minced Beef at Retail.” Journal of Applied Microbiology 108 (1).
APA
De Smet, Sarah, De Zutter, L., Van Hende, J., & Houf, K. (2010). Arcobacter contamination on pre- and post-chilled bovine carcasses and in minced beef at retail. JOURNAL OF APPLIED MICROBIOLOGY, 108(1).
Vancouver
1.
De Smet S, De Zutter L, Van Hende J, Houf K. Arcobacter contamination on pre- and post-chilled bovine carcasses and in minced beef at retail. JOURNAL OF APPLIED MICROBIOLOGY. 2010;108(1).
MLA
De Smet, Sarah, Lieven De Zutter, Johan Van Hende, et al. “Arcobacter Contamination on Pre- and Post-chilled Bovine Carcasses and in Minced Beef at Retail.” JOURNAL OF APPLIED MICROBIOLOGY 108.1 (2010): n. pag. Print.