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Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods

(2010) PHOTOSYNTHESIS RESEARCH. 104(2-3). p.153-162
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Abstract
Chlorobium tepidum is a Gram-negative bacterium of the green sulfur phylum (Chlorobia). Chlorobia are obligate anaerobic photolithoautotrophs that are widely distributed in aquatic environments where anoxic layers containing reduced sulfur compounds are exposed to light. The envelope of C. tepidum is a complex organelle composed of the outer membrane, the periplasm-peptidoglycan layer, and the cytoplasmic membrane. In addition to the outer and plasma membranes, C. tepidum contains chlorosomes attached to the cytoplasmic side of the plasma membrane. Each cellular compartment has a unique set of proteins, called sub-proteome. An important aim of proteome analysis is to study the level of the expressed genes and their response to environmental changes. Membrane protein studies are of primary importance to understand how nutrients are transported inside the cell, how toxic molecules are exported, and the mechanisms of photosynthesis and energy metabolism.
Keywords
FUNCTIONAL PROTEOMICS, 2-DIMENSIONAL ELECTROPHORESIS, OUTER-MEMBRANE, BLUE-NATIVE ELECTROPHORESIS, COMPLETE GENOME SEQUENCE, Photosynthesis, Inner membrane, ELECTRON-MICROSCOPY, Outer membrane, Mass spectrometric identification, Chlorobium tepidum, Membrane proteins, Two-dimensional polyacryamide gel electrophoresis, COFRADIC, Blue native, Green sulfur bacteria, Chlorosome, PROTEINS, COMPLEXES, TOOL, CHROMATOGRAPHY

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Chicago
Kouyianou, Kalliopi, Michalis Aivaliotis, Kris Gevaert, Michael Karas, and Georgios Tsiotis. 2010. “Membrane Proteome of the Green Sulfur Bacterium Chlorobium Tepidum (syn. Chlorobaculum Tepidum) Analyzed by Gel-based and Gel-free Methods.” Photosynthesis Research 104 (2-3): 153–162.
APA
Kouyianou, Kalliopi, Aivaliotis, M., Gevaert, K., Karas, M., & Tsiotis, G. (2010). Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods. PHOTOSYNTHESIS RESEARCH, 104(2-3), 153–162.
Vancouver
1.
Kouyianou K, Aivaliotis M, Gevaert K, Karas M, Tsiotis G. Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods. PHOTOSYNTHESIS RESEARCH. 2010;104(2-3):153–62.
MLA
Kouyianou, Kalliopi, Michalis Aivaliotis, Kris Gevaert, et al. “Membrane Proteome of the Green Sulfur Bacterium Chlorobium Tepidum (syn. Chlorobaculum Tepidum) Analyzed by Gel-based and Gel-free Methods.” PHOTOSYNTHESIS RESEARCH 104.2-3 (2010): 153–162. Print.
@article{998221,
  abstract     = {Chlorobium tepidum is a Gram-negative bacterium of the green sulfur phylum (Chlorobia). Chlorobia are obligate anaerobic photolithoautotrophs that are widely distributed in aquatic environments where anoxic layers containing reduced sulfur compounds are exposed to light. The envelope of C. tepidum is a complex organelle composed of the outer membrane, the periplasm-peptidoglycan layer, and the cytoplasmic membrane. In addition to the outer and plasma membranes, C. tepidum contains chlorosomes attached to the cytoplasmic side of the plasma membrane. Each cellular compartment has a unique set of proteins, called sub-proteome. An important aim of proteome analysis is to study the level of the expressed genes and their response to environmental changes. Membrane protein studies are of primary importance to understand how nutrients are transported inside the cell, how toxic molecules are exported, and the mechanisms of photosynthesis and energy metabolism.},
  author       = {Kouyianou, Kalliopi and Aivaliotis, Michalis and Gevaert, Kris and Karas, Michael and Tsiotis, Georgios},
  issn         = {0166-8595},
  journal      = {PHOTOSYNTHESIS RESEARCH},
  keyword      = {FUNCTIONAL PROTEOMICS,2-DIMENSIONAL ELECTROPHORESIS,OUTER-MEMBRANE,BLUE-NATIVE ELECTROPHORESIS,COMPLETE GENOME SEQUENCE,Photosynthesis,Inner membrane,ELECTRON-MICROSCOPY,Outer membrane,Mass spectrometric identification,Chlorobium tepidum,Membrane proteins,Two-dimensional polyacryamide gel electrophoresis,COFRADIC,Blue native,Green sulfur bacteria,Chlorosome,PROTEINS,COMPLEXES,TOOL,CHROMATOGRAPHY},
  language     = {eng},
  number       = {2-3},
  pages        = {153--162},
  title        = {Membrane proteome of the green sulfur bacterium Chlorobium tepidum (syn. Chlorobaculum tepidum) analyzed by gel-based and gel-free methods},
  url          = {http://dx.doi.org/10.1007/s11120-010-9544-x},
  volume       = {104},
  year         = {2010},
}

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