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A new preparation method to study fresh plant structures with X-ray computed tomography

Olivier Leroux (UGent) , F Leroux, Elke Bellefroid (UGent) , Myriam Claeys (UGent) , Marjolein Couvreur (UGent) , Gaetan Borgonie (UGent) , Luc Van Hoorebeke (UGent) , Bert Masschaele (UGent) and Ronnie Viane (UGent)
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Abstract
Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.
Keywords
WOOD DENSITY, PODOCARPACEAE, SEEDS, ACMOPYLE-PANCHERI, WATER-CONTENT, PHASE-CONTRAST, FEMALE CONES, MICROTOMOGRAPHY, ROOTS, VISUALIZATION, 3-D, sample preparation, fern root, en bloc staining, critical point drying, Computed tomography

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Citation

Please use this url to cite or link to this publication:

MLA
Leroux, Olivier et al. “A New Preparation Method to Study Fresh Plant Structures with X-ray Computed Tomography.” JOURNAL OF MICROSCOPY-OXFORD 233.1 (2009): 1–4. Print.
APA
Leroux, O., Leroux, F., Bellefroid, E., Claeys, M., Couvreur, M., Borgonie, G., Van Hoorebeke, L., et al. (2009). A new preparation method to study fresh plant structures with X-ray computed tomography. JOURNAL OF MICROSCOPY-OXFORD, 233(1), 1–4.
Chicago author-date
Leroux, Olivier, F Leroux, Elke Bellefroid, Myriam Claeys, Marjolein Couvreur, Gaetan Borgonie, Luc Van Hoorebeke, Bert Masschaele, and Ronnie Viane. 2009. “A New Preparation Method to Study Fresh Plant Structures with X-ray Computed Tomography.” Journal of Microscopy-oxford 233 (1): 1–4.
Chicago author-date (all authors)
Leroux, Olivier, F Leroux, Elke Bellefroid, Myriam Claeys, Marjolein Couvreur, Gaetan Borgonie, Luc Van Hoorebeke, Bert Masschaele, and Ronnie Viane. 2009. “A New Preparation Method to Study Fresh Plant Structures with X-ray Computed Tomography.” Journal of Microscopy-oxford 233 (1): 1–4.
Vancouver
1.
Leroux O, Leroux F, Bellefroid E, Claeys M, Couvreur M, Borgonie G, et al. A new preparation method to study fresh plant structures with X-ray computed tomography. JOURNAL OF MICROSCOPY-OXFORD. 2009;233(1):1–4.
IEEE
[1]
O. Leroux et al., “A new preparation method to study fresh plant structures with X-ray computed tomography,” JOURNAL OF MICROSCOPY-OXFORD, vol. 233, no. 1, pp. 1–4, 2009.
@article{996978,
  abstract     = {Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.},
  author       = {Leroux, Olivier and Leroux, F and Bellefroid, Elke and Claeys, Myriam and Couvreur, Marjolein and Borgonie, Gaetan and Van Hoorebeke, Luc and Masschaele, Bert and Viane, Ronnie},
  issn         = {0022-2720},
  journal      = {JOURNAL OF MICROSCOPY-OXFORD},
  keywords     = {WOOD DENSITY,PODOCARPACEAE,SEEDS,ACMOPYLE-PANCHERI,WATER-CONTENT,PHASE-CONTRAST,FEMALE CONES,MICROTOMOGRAPHY,ROOTS,VISUALIZATION,3-D,sample preparation,fern root,en bloc staining,critical point drying,Computed tomography},
  language     = {eng},
  number       = {1},
  pages        = {1--4},
  title        = {A new preparation method to study fresh plant structures with X-ray computed tomography},
  url          = {http://dx.doi.org/10.1111/j.1365-2818.2008.03088.x},
  volume       = {233},
  year         = {2009},
}

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