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Birth of the first ICSI foal in the Benelux

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Organization
Abstract
This paper describes the birth of the first intracytoplasmic sperm injection (ICSI) foal in the Benelux. Oocytes were aspirated from ovaries from slaughtered mares. After in vitro maturation, the oocytes were fertilized by ICSI and cultured in vitro for 9 days. Two embryos reached the blastocyst stage and they were transferred to the uterus of a synchronized mare. Six days later a single embryonic vesicle was diagnosed by ultrasound. After a normal pregnancy a healthy foal was born on the 27(th) of October 2009. Parentage testing via microsatellite genotyping confirmed that the foal originated from the transferred embryo.
Keywords
VIVO, BLASTOCYSTS, OOCYTES, FROZEN-THAWED SPERMATOZOA, IN-VITRO, INTRACYTOPLASMIC SPERM INJECTION, DEVELOPMENTAL COMPETENCE, EQUINE EMBRYOS

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Citation

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MLA
Smits, Katrien et al. “Birth of the First ICSI Foal in the Benelux.” Vlaams Diergeneeskundig Tijdschrift 79.2 (2010): 134–138. Print.
APA
Smits, K., Govaere, J., Hoogewijs, M., De Schauwer, C., Van Haesebrouck, E., Van Poucke, M., Peelman, L., et al. (2010). Birth of the first ICSI foal in the Benelux. Vlaams Diergeneeskundig Tijdschrift, 79(2), 134–138.
Chicago author-date
Smits, Katrien, Jan Govaere, Maarten Hoogewijs, Catharina De Schauwer, E Van Haesebrouck, Mario Van Poucke, Luc Peelman, M van den Berg, T Vullers, and Ann Van Soom. 2010. “Birth of the First ICSI Foal in the Benelux.” Vlaams Diergeneeskundig Tijdschrift 79 (2): 134–138.
Chicago author-date (all authors)
Smits, Katrien, Jan Govaere, Maarten Hoogewijs, Catharina De Schauwer, E Van Haesebrouck, Mario Van Poucke, Luc Peelman, M van den Berg, T Vullers, and Ann Van Soom. 2010. “Birth of the First ICSI Foal in the Benelux.” Vlaams Diergeneeskundig Tijdschrift 79 (2): 134–138.
Vancouver
1.
Smits K, Govaere J, Hoogewijs M, De Schauwer C, Van Haesebrouck E, Van Poucke M, et al. Birth of the first ICSI foal in the Benelux. Vlaams Diergeneeskundig Tijdschrift. FACULTY VETERINARY MEDICINE, B-9000 GHENT, BELGIUM: UNIV GHENT; 2010;79(2):134–8.
IEEE
[1]
K. Smits et al., “Birth of the first ICSI foal in the Benelux,” Vlaams Diergeneeskundig Tijdschrift, vol. 79, no. 2, pp. 134–138, 2010.
@article{996203,
  abstract     = {This paper describes the birth of the first intracytoplasmic sperm injection (ICSI) foal in the Benelux. Oocytes were aspirated from ovaries from slaughtered mares. After in vitro maturation, the oocytes were fertilized by ICSI and cultured in vitro for 9 days. Two embryos reached the blastocyst stage and they were transferred to the uterus of a synchronized mare. Six days later a single embryonic vesicle was diagnosed by ultrasound. After a normal pregnancy a healthy foal was born on the 27(th) of October 2009. Parentage testing via microsatellite genotyping confirmed that the foal originated from the transferred embryo.},
  author       = {Smits, Katrien and Govaere, Jan and Hoogewijs, Maarten and De Schauwer, Catharina and Van Haesebrouck, E and Van Poucke, Mario and Peelman, Luc and van den Berg, M and Vullers, T and Van Soom, Ann},
  issn         = {0303-9021},
  journal      = {Vlaams Diergeneeskundig Tijdschrift},
  keywords     = {VIVO,BLASTOCYSTS,OOCYTES,FROZEN-THAWED SPERMATOZOA,IN-VITRO,INTRACYTOPLASMIC SPERM INJECTION,DEVELOPMENTAL COMPETENCE,EQUINE EMBRYOS},
  language     = {eng},
  number       = {2},
  pages        = {134--138},
  publisher    = {UNIV GHENT},
  title        = {Birth of the first ICSI foal in the Benelux},
  volume       = {79},
  year         = {2010},
}

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