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Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy

Winnok De Vos UGent, GH Joss, W Haffmans, RA Hoebe, EMM Manders and Patric Van Oostveldt UGent (2010) JOURNAL OF MICROSCOPY-OXFORD. 238(3). p.254-264
abstract
Telomeres are the complex end structures that confer functional integrity and positional stability to human chromosomes. Telomere research has long been dominated by length measurements and biochemical analyses. Recently, interest has shifted towards the role of their three-dimensional organization and dynamics within the nuclear volume. In the mammalian interphase nucleus, there is increasing evidence for a telomeric configuration that is non-random and is cell cycle and cell type dependent. This has functional implications for genome stability. Objective and reproducible representation of the spatiotemporal organization of telomeres, under different experimental conditions, requires quantification by reliable automated image processing techniques. In this paper, we describe methods for quantitative telomere analysis in cell nuclei of living human cells expressing telomere-binding fusion proteins. We present a toolbox for determining telomere positions within the nucleus with subresolution accuracy and tracking telomeres in 4D controlled light exposure microscopy (CLEM) recordings. The use of CLEM allowed for durable imaging and thereby improved segmentation performance considerably. With minor modifications, the underlying algorithms can be expanded to the analysis of other intranuclear features, such as nuclear bodies or DNA double stranded break foci.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MAMMALIAN-CELLS, ECV304, 3D, MOBILITY, NUCLEI, REVEALS, ARCHITECTURE, MOTION, image analysis, telomeres, CLEM, SPOTS, DYNAMICS
journal title
JOURNAL OF MICROSCOPY-OXFORD
J. Microsc.-Oxf.
volume
238
issue
3
pages
254 - 264
Web of Science type
Article
Web of Science id
000277867900007
JCR category
MICROSCOPY
JCR impact factor
1.872 (2010)
JCR rank
4/9 (2010)
JCR quartile
2 (2010)
ISSN
0022-2720
DOI
10.1111/j.1365-2818.2009.03350.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
987486
handle
http://hdl.handle.net/1854/LU-987486
date created
2010-06-25 17:23:52
date last changed
2016-12-19 15:42:12
@article{987486,
  abstract     = {Telomeres are the complex end structures that confer functional integrity and positional stability to human chromosomes. Telomere research has long been dominated by length measurements and biochemical analyses. Recently, interest has shifted towards the role of their three-dimensional organization and dynamics within the nuclear volume. In the mammalian interphase nucleus, there is increasing evidence for a telomeric configuration that is non-random and is cell cycle and cell type dependent. This has functional implications for genome stability. Objective and reproducible representation of the spatiotemporal organization of telomeres, under different experimental conditions, requires quantification by reliable automated image processing techniques. In this paper, we describe methods for quantitative telomere analysis in cell nuclei of living human cells expressing telomere-binding fusion proteins. We present a toolbox for determining telomere positions within the nucleus with subresolution accuracy and tracking telomeres in 4D controlled light exposure microscopy (CLEM) recordings. The use of CLEM allowed for durable imaging and thereby improved segmentation performance considerably. With minor modifications, the underlying algorithms can be expanded to the analysis of other intranuclear features, such as nuclear bodies or DNA double stranded break foci.},
  author       = {De Vos, Winnok and Joss, GH and Haffmans, W and Hoebe, RA and Manders, EMM and Van Oostveldt, Patric},
  issn         = {0022-2720},
  journal      = {JOURNAL OF MICROSCOPY-OXFORD},
  keyword      = {MAMMALIAN-CELLS,ECV304,3D,MOBILITY,NUCLEI,REVEALS,ARCHITECTURE,MOTION,image analysis,telomeres,CLEM,SPOTS,DYNAMICS},
  language     = {eng},
  number       = {3},
  pages        = {254--264},
  title        = {Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy},
  url          = {http://dx.doi.org/10.1111/j.1365-2818.2009.03350.x},
  volume       = {238},
  year         = {2010},
}

Chicago
De Vos, Winnok, GH Joss, W Haffmans, RA Hoebe, EMM Manders, and Patric Van Oostveldt. 2010. “Four-dimensional Telomere Analysis in Recordings of Living Human Cells Acquired with Controlled Light Exposure Microscopy.” Journal of Microscopy-oxford 238 (3): 254–264.
APA
De Vos, Winnok, Joss, G., Haffmans, W., Hoebe, R., Manders, E., & Van Oostveldt, P. (2010). Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy. JOURNAL OF MICROSCOPY-OXFORD, 238(3), 254–264.
Vancouver
1.
De Vos W, Joss G, Haffmans W, Hoebe R, Manders E, Van Oostveldt P. Four-dimensional telomere analysis in recordings of living human cells acquired with Controlled Light Exposure Microscopy. JOURNAL OF MICROSCOPY-OXFORD. 2010;238(3):254–64.
MLA
De Vos, Winnok, GH Joss, W Haffmans, et al. “Four-dimensional Telomere Analysis in Recordings of Living Human Cells Acquired with Controlled Light Exposure Microscopy.” JOURNAL OF MICROSCOPY-OXFORD 238.3 (2010): 254–264. Print.