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Layer-by-layer incorporation of growth factors in decellularized aortic heart valve leaflets

Liesbeth De Cock UGent, Stefaan De Koker UGent, Filip De Vos UGent, Chris Vervaet UGent, Jean Paul Remon UGent and Bruno De Geest UGent (2010) BIOMACROMOLECULES. 11(4). p.1002-1008
abstract
Aortic heart valve disease is a growing health problem and a tissue-engineered aortic heart valve could be a promising therapy. In this paper, clecellularized porcine aortic heart valve leaflets are used as scaffolds and loaded with growth factor and heparin via layer-by-layer electrostatic deposition (LbL technique) with the final purpose to stimulate and control cellular processes. Binding and subsequent release of heparin and basic fibroblast growth factor (bFGF) from aortic valve leaflets were assessed qualitatively by immunohistochemistry and quantitatively by radioactive labeling methods. It was observed that the amount of heparin and bFGF bound to aortic heart valve leaflets was directly proportional to the concentration of heparin and bFGF in the incubation medium. Release of heparin and bFGF from the decellularized heart valve leaflets at physiological conditions was sustained over 4 days while preserving the biological activity of the released growth factor.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PROTEIN, SURFACE, GLYCOSAMINOGLYCANS, EXTRACELLULAR-MATRIX, BOVINE SERUM-ALBUMIN, COLLAGEN MATRICES, CELL, HEPARIN-BINDING, DELIVERY, SCAFFOLDS
journal title
BIOMACROMOLECULES
Biomacromolecules
volume
11
issue
4
pages
1002 - 1008
Web of Science type
Article
Web of Science id
000276557300023
JCR category
POLYMER SCIENCE
JCR impact factor
5.325 (2010)
JCR rank
4/78 (2010)
JCR quartile
1 (2010)
ISSN
1525-7797
DOI
10.1021/bm9014649
language
English
UGent publication?
yes
classification
A1
id
986065
handle
http://hdl.handle.net/1854/LU-986065
date created
2010-06-24 14:58:32
date last changed
2010-07-29 14:04:55
@article{986065,
  abstract     = {Aortic heart valve disease is a growing health problem and a tissue-engineered aortic heart valve could be a promising therapy. In this paper, clecellularized porcine aortic heart valve leaflets are used as scaffolds and loaded with growth factor and heparin via layer-by-layer electrostatic deposition (LbL technique) with the final purpose to stimulate and control cellular processes. Binding and subsequent release of heparin and basic fibroblast growth factor (bFGF) from aortic valve leaflets were assessed qualitatively by immunohistochemistry and quantitatively by radioactive labeling methods. It was observed that the amount of heparin and bFGF bound to aortic heart valve leaflets was directly proportional to the concentration of heparin and bFGF in the incubation medium. Release of heparin and bFGF from the decellularized heart valve leaflets at physiological conditions was sustained over 4 days while preserving the biological activity of the released growth factor.},
  author       = {De Cock, Liesbeth and De Koker, Stefaan and De Vos, Filip and Vervaet, Chris and Remon, Jean Paul and De Geest, Bruno},
  issn         = {1525-7797},
  journal      = {BIOMACROMOLECULES},
  keyword      = {PROTEIN,SURFACE,GLYCOSAMINOGLYCANS,EXTRACELLULAR-MATRIX,BOVINE SERUM-ALBUMIN,COLLAGEN MATRICES,CELL,HEPARIN-BINDING,DELIVERY,SCAFFOLDS},
  language     = {eng},
  number       = {4},
  pages        = {1002--1008},
  title        = {Layer-by-layer incorporation of growth factors in decellularized aortic heart valve leaflets},
  url          = {http://dx.doi.org/10.1021/bm9014649},
  volume       = {11},
  year         = {2010},
}

Chicago
De Cock, Liesbeth, Stefaan De Koker, Filip De Vos, Chris Vervaet, Jean Paul Remon, and Bruno De Geest. 2010. “Layer-by-layer Incorporation of Growth Factors in Decellularized Aortic Heart Valve Leaflets.” Biomacromolecules 11 (4): 1002–1008.
APA
De Cock, Liesbeth, De Koker, S., De Vos, F., Vervaet, C., Remon, J. P., & De Geest, B. (2010). Layer-by-layer incorporation of growth factors in decellularized aortic heart valve leaflets. BIOMACROMOLECULES, 11(4), 1002–1008.
Vancouver
1.
De Cock L, De Koker S, De Vos F, Vervaet C, Remon JP, De Geest B. Layer-by-layer incorporation of growth factors in decellularized aortic heart valve leaflets. BIOMACROMOLECULES. 2010;11(4):1002–8.
MLA
De Cock, Liesbeth, Stefaan De Koker, Filip De Vos, et al. “Layer-by-layer Incorporation of Growth Factors in Decellularized Aortic Heart Valve Leaflets.” BIOMACROMOLECULES 11.4 (2010): 1002–1008. Print.