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A bio-analytical hydrophilic interaction LC-MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children

Julie De Smet UGent, Koen Boussery UGent, Pieter De Cock UGent, Peter De Paepe UGent, Jean Paul Remon UGent, Myriam Van Winckel UGent and Jan Van Bocxlaer UGent (2010) JOURNAL OF SEPARATION SCIENCE. 33(6-7). p.939-947
abstract
A hydrophilic interaction LC method with MS/MS was developed and validated for the simultaneous quantification of omeprazole and lansoprazole in human plasma. Chromatographic separation was achieved on a Betasil silica column using a high organic mobile phase (eluent A: ACN/formic acid 997.5:2.5 v/v; eluent B: water/formic acid 997.5:2.5 v/v) and gradient elution. The mass spectrometer was operated in the Multiple Reaction Monitoring mode. Prior to chromatography, liquid-liquid extraction with ethyl acetate was used and the organic layer was diluted with ACN, allowing direct injection on column. The method showed acceptable linearity, high precision (RSD%<10.5%), accuracy (88.9-109.3%) and selectivity in the two concentration ranges studied: 1.5-100 and 5-2000 ng/mL. The LOQ was established at 1.5 and 5 ng/mL for the two concentration ranges. Lack of variability in matrix effects was demonstrated and mean extraction recovery for omeprazole and lansoprazole was determined in the low (56.3-67.7%) and high (45.3-44.3%) concentration range, respectively. Additionally, plasma samples were found to be stable after three freeze-thaw cycles and for at least 15 h after extraction. This assay was successfully applied to a pharmacokinetic omeprazole study in children with cerebral palsy and mental retardation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PERFORMANCE LIQUID-CHROMATOGRAPHY, PROTON PUMP INHIBITORS, HPLC, TANDEM MASS-SPECTROMETRY, Quantification, Proton pump inhibitor, Omeprazole, Lansoprazole, SOLID-PHASE EXTRACTION, Hydrophilic interaction LC, METABOLITES, BLOOD-PLASMA, PANTOPRAZOLE, COLUMN, SINGLE
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
33
issue
6-7
pages
939 - 947
Web of Science type
Article
Web of Science id
000276782700028
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.631 (2010)
JCR rank
23/71 (2010)
JCR quartile
2 (2010)
ISSN
1615-9306
DOI
10.1002/jssc.200900590
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
986003
handle
http://hdl.handle.net/1854/LU-986003
date created
2010-06-24 14:38:55
date last changed
2010-08-19 11:28:02
@article{986003,
  abstract     = {A hydrophilic interaction LC method with MS/MS was developed and validated for the simultaneous quantification of omeprazole and lansoprazole in human plasma. Chromatographic separation was achieved on a Betasil silica column using a high organic mobile phase (eluent A: ACN/formic acid 997.5:2.5 v/v; eluent B: water/formic acid 997.5:2.5 v/v) and gradient elution. The mass spectrometer was operated in the Multiple Reaction Monitoring mode. Prior to chromatography, liquid-liquid extraction with ethyl acetate was used and the organic layer was diluted with ACN, allowing direct injection on column. The method showed acceptable linearity, high precision (RSD\%{\textlangle}10.5\%), accuracy (88.9-109.3\%) and selectivity in the two concentration ranges studied: 1.5-100 and 5-2000 ng/mL. The LOQ was established at 1.5 and 5 ng/mL for the two concentration ranges. Lack of variability in matrix effects was demonstrated and mean extraction recovery for omeprazole and lansoprazole was determined in the low (56.3-67.7\%) and high (45.3-44.3\%) concentration range, respectively. Additionally, plasma samples were found to be stable after three freeze-thaw cycles and for at least 15 h after extraction. This assay was successfully applied to a pharmacokinetic omeprazole study in children with cerebral palsy and mental retardation.},
  author       = {De Smet, Julie and Boussery, Koen and De Cock, Pieter and De Paepe, Peter and Remon, Jean Paul and Van Winckel, Myriam and Van Bocxlaer, Jan},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {PERFORMANCE LIQUID-CHROMATOGRAPHY,PROTON PUMP INHIBITORS,HPLC,TANDEM MASS-SPECTROMETRY,Quantification,Proton pump inhibitor,Omeprazole,Lansoprazole,SOLID-PHASE EXTRACTION,Hydrophilic interaction LC,METABOLITES,BLOOD-PLASMA,PANTOPRAZOLE,COLUMN,SINGLE},
  language     = {eng},
  number       = {6-7},
  pages        = {939--947},
  title        = {A bio-analytical hydrophilic interaction LC-MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children},
  url          = {http://dx.doi.org/10.1002/jssc.200900590},
  volume       = {33},
  year         = {2010},
}

Chicago
De Smet, Julie, Koen Boussery, Pieter De Cock, Peter De Paepe, Jean Paul Remon, Myriam Van Winckel, and Jan Van Bocxlaer. 2010. “A Bio-analytical Hydrophilic Interaction LC-MS/MS Method for the Simultaneous Quantification of Omeprazole and Lansoprazole in Human Plasma in Support of a Pharmacokinetic Omeprazole Study in Children.” Journal of Separation Science 33 (6-7): 939–947.
APA
De Smet, Julie, Boussery, K., De Cock, P., De Paepe, P., Remon, J. P., Van Winckel, M., & Van Bocxlaer, J. (2010). A bio-analytical hydrophilic interaction LC-MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children. JOURNAL OF SEPARATION SCIENCE, 33(6-7), 939–947.
Vancouver
1.
De Smet J, Boussery K, De Cock P, De Paepe P, Remon JP, Van Winckel M, et al. A bio-analytical hydrophilic interaction LC-MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children. JOURNAL OF SEPARATION SCIENCE. 2010;33(6-7):939–47.
MLA
De Smet, Julie, Koen Boussery, Pieter De Cock, et al. “A Bio-analytical Hydrophilic Interaction LC-MS/MS Method for the Simultaneous Quantification of Omeprazole and Lansoprazole in Human Plasma in Support of a Pharmacokinetic Omeprazole Study in Children.” JOURNAL OF SEPARATION SCIENCE 33.6-7 (2010): 939–947. Print.