Ghent University Academic Bibliography

Advanced

Influence of cryopreservation on the pulpal tissue of immature third molars in vitro.

Liesbeth Temmerman UGent, Hilde Beele UGent, Luc Dermaut UGent, GEORGES VAN MAELE UGent and Guy De Pauw UGent (2010) CELL AND TISSUE BANKING. 11(3). p.281-289
abstract
The purpose of this study was to evaluate in vitro the viability of isolated and non-isolated pulpal tissue of immature third molars after cryopreservation. This study was divided in three different experiments. Experiment 1: Pulpal tissue isolated from 19 third molars was divided in horizontal segments. Each segment was cultured separately in order to evaluate whether differences in growth capacity within the tissue could be found. Experiment 2: Pulpal tissue isolated from 27 third molars was divided in a mesial and a distal part. One part was cryopreserved before culturing, the other part was cultured immediately. Growth capacity of cryopreserved and non-cryopreserved tissue was evaluated and compared. Experiment 3: 43 third molars were cryopreserved. After thawing, the dimension of the apical foramen was measured and the pulp was isolated and segmented horizontally. The different parts were cultured and growth capacity was evaluated and compared. Results of experiment 1 and 2 showed no significant difference in growth capacity between fibroblasts originating from different pulpal segments of the same tooth without cryopreservation and between fibroblasts originating from cryopreserved and non-cryopreserved isolated pulpal tissue. In experiment 3 it was demonstrated that the dimension of the apical foramen and pulpal viability after cryopreservation are positively correlated. A minimum dimension of 9.42 mm(2) enables the cryoprotective agent to penetrate sufficiently and to protect the pulpal tissue from apex to crown. This study proved that cryopreservation of human pulpal tissue is possible if the cryoprotective agent can reach the entire pulp.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Viability, Apical foramen, PERIODONTAL REGENERATION, MATURE TEETH, REPLANTATION, AUTOTRANSPLANTATION, FIBROBLASTS, TOOTH, MONKEYS, Autotransplantation, Cryopreservation, Cell culture, Pulpal Tissue
journal title
CELL AND TISSUE BANKING
Cell Tissue Banking
volume
11
issue
3
pages
281 - 289
Web of Science type
Article
Web of Science id
000279295000008
JCR category
ENGINEERING, BIOMEDICAL
JCR impact factor
1.157 (2010)
JCR rank
45/68 (2010)
JCR quartile
3 (2010)
ISSN
1389-9333
DOI
10.1007/s10561-009-9148-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
984718
handle
http://hdl.handle.net/1854/LU-984718
date created
2010-06-23 12:19:40
date last changed
2010-09-16 13:03:54
@article{984718,
  abstract     = {The purpose of this study was to evaluate in vitro the viability of isolated and non-isolated pulpal tissue of immature third molars after cryopreservation. This study was divided in three different experiments. Experiment 1: Pulpal tissue isolated from 19 third molars was divided in horizontal segments. Each segment was cultured separately in order to evaluate whether differences in growth capacity within the tissue could be found. Experiment 2: Pulpal tissue isolated from 27 third molars was divided in a mesial and a distal part. One part was cryopreserved before culturing, the other part was cultured immediately. Growth capacity of cryopreserved and non-cryopreserved tissue was evaluated and compared. Experiment 3: 43 third molars were cryopreserved. After thawing, the dimension of the apical foramen was measured and the pulp was isolated and segmented horizontally. The different parts were cultured and growth capacity was evaluated and compared. Results of experiment 1 and 2 showed no significant difference in growth capacity between fibroblasts originating from different pulpal segments of the same tooth without cryopreservation and between fibroblasts originating from cryopreserved and non-cryopreserved isolated pulpal tissue. In experiment 3 it was demonstrated that the dimension of the apical foramen and pulpal viability after cryopreservation are positively correlated. A minimum dimension of 9.42 mm(2) enables the cryoprotective agent to penetrate sufficiently and to protect the pulpal tissue from apex to crown. This study proved that cryopreservation of human pulpal tissue is possible if the cryoprotective agent can reach the entire pulp.},
  author       = {Temmerman, Liesbeth and Beele, Hilde and Dermaut, Luc and VAN MAELE, GEORGES and De Pauw, Guy},
  issn         = {1389-9333},
  journal      = {CELL AND TISSUE BANKING},
  keyword      = {Viability,Apical foramen,PERIODONTAL REGENERATION,MATURE TEETH,REPLANTATION,AUTOTRANSPLANTATION,FIBROBLASTS,TOOTH,MONKEYS,Autotransplantation,Cryopreservation,Cell culture,Pulpal Tissue},
  language     = {eng},
  number       = {3},
  pages        = {281--289},
  title        = {Influence of cryopreservation on the pulpal tissue of immature third molars in vitro.},
  url          = {http://dx.doi.org/10.1007/s10561-009-9148-x},
  volume       = {11},
  year         = {2010},
}

Chicago
Temmerman, Liesbeth, Hilde Beele, Luc Dermaut, Georges Van Maele, and Guy De Pauw. 2010. “Influence of Cryopreservation on the Pulpal Tissue of Immature Third Molars in Vitro.” Cell and Tissue Banking 11 (3): 281–289.
APA
Temmerman, Liesbeth, Beele, H., Dermaut, L., Van Maele, G., & De Pauw, G. (2010). Influence of cryopreservation on the pulpal tissue of immature third molars in vitro. CELL AND TISSUE BANKING, 11(3), 281–289.
Vancouver
1.
Temmerman L, Beele H, Dermaut L, Van Maele G, De Pauw G. Influence of cryopreservation on the pulpal tissue of immature third molars in vitro. CELL AND TISSUE BANKING. 2010;11(3):281–9.
MLA
Temmerman, Liesbeth, Hilde Beele, Luc Dermaut, et al. “Influence of Cryopreservation on the Pulpal Tissue of Immature Third Molars in Vitro.” CELL AND TISSUE BANKING 11.3 (2010): 281–289. Print.