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Disposition of soy isoflavones in normal human breast tissue

Selin Bolca UGent, Mireia Urpi-Sarda, Phillip Blondeel UGent, Nathalie Roche UGent, Lynn Vanhaecke UGent, Sam Possemiers UGent, Nawaf Al-Maharik, Nigel Botting, Denis De Keukeleire UGent and Marc Bracke UGent, et al. (2010) AMERICAN JOURNAL OF CLINICAL NUTRITION. 91(4). p.976-984
abstract
Background: Despite decades of research on the relation between soy and breast cancer, questions regarding the absorption, metabolism, and distribution of isoflavones in breast tissue largely remain unanswered. Objective: We evaluated the potential health effects of isoflavone consumption on normal breast tissue; isoflavone concentrations, metabolites, and biodistribution were investigated and compared with 17 beta-estradiol exposure. Design: In this dietary intervention study, healthy women were randomly allocated to a soy milk (n = 11; 16.98-mg genistein and 5.40-mg daidzein aglycone equivalents per dose), soy supplement (n = 10; 5.27-mg genistein and 17.56-mg daidzein aglycone equivalents per dose), or control (n = 10) group. After a run-in period >= 4 d, 3 doses of soy milk or soy supplements were taken daily for 5 d before an esthetic breast reduction. Blood and breast biopsies were collected during surgery and analyzed with liquid chromatography-tandem mass spectrometry. Results: After soy administration, genistein and total daidzein concentrations, which were expressed as aglycone equivalents, ranged from 135.1 to 2831 nmol/L and 105.1 to 1397 nmol/L, respectively, in hydrolyzed serum and from 92.33 to 493.8 pmol/g and 22.15 to 770.8 pmol/g, respectively, in hydrolyzed breast tissue. The major metabolites identified in nonhydrolyzed samples were genistein-7-O-glucuronide and daidzein-7-O-glucuronide, with an overall glucuronidation of 98%. Total isoflavones showed a breast adipose/glandular tissue distribution of 40: 60, and their mean (+/- SEM) derived 17 beta-estradiol equivalents toward estrogen receptor beta were 21 +/- 4-fold and 40 +/- 10-fold higher than the 17 beta-estradiol concentrations in adipose (0.283 +/- 6 0.089 pmol/g, P < 0.001) and glandular (0.246 +/- 0.091 pmol/g, P = 0.001) fractions, respectively. Conclusion: After intake of soy milk and soy supplements, isoflavones reach exposure levels in breast tissue at which potential health effects may occur.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MAMMARY-GLAND, MCF-7 TUMORS, STIMULATE GROWTH, AROMATASE ACTIVITIES, IN-VITRO, POSTMENOPAUSAL WOMEN, ESTROGEN-RECEPTOR-ALPHA, CANCER TUMORS, HUMAN URINE, PHYTOESTROGENS
journal title
AMERICAN JOURNAL OF CLINICAL NUTRITION
Am. J. Clin. Nutr.
volume
91
issue
4
pages
976 - 984
Web of Science type
Article
Web of Science id
000275802400019
JCR category
NUTRITION & DIETETICS
JCR impact factor
6.606 (2010)
JCR rank
3/70 (2010)
JCR quartile
1 (2010)
ISSN
0002-9165
DOI
10.3945/ajcn.2009.28854
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
980671
handle
http://hdl.handle.net/1854/LU-980671
date created
2010-06-18 08:52:57
date last changed
2011-07-14 10:05:35
@article{980671,
  abstract     = {Background: Despite decades of research on the relation between soy and breast cancer, questions regarding the absorption, metabolism, and distribution of isoflavones in breast tissue largely remain unanswered.
Objective: We evaluated the potential health effects of isoflavone consumption on normal breast tissue; isoflavone concentrations, metabolites, and biodistribution were investigated and compared with 17 beta-estradiol exposure.
Design: In this dietary intervention study, healthy women were randomly allocated to a soy milk (n = 11; 16.98-mg genistein and 5.40-mg daidzein aglycone equivalents per dose), soy supplement (n = 10; 5.27-mg genistein and 17.56-mg daidzein aglycone equivalents per dose), or control (n = 10) group. After a run-in period {\textrangle}= 4 d, 3 doses of soy milk or soy supplements were taken daily for 5 d before an esthetic breast reduction. Blood and breast biopsies were collected during surgery and analyzed with liquid chromatography-tandem mass spectrometry.
Results: After soy administration, genistein and total daidzein concentrations, which were expressed as aglycone equivalents, ranged from 135.1 to 2831 nmol/L and 105.1 to 1397 nmol/L, respectively, in hydrolyzed serum and from 92.33 to 493.8 pmol/g and 22.15 to 770.8 pmol/g, respectively, in hydrolyzed breast tissue. The major metabolites identified in nonhydrolyzed samples were genistein-7-O-glucuronide and daidzein-7-O-glucuronide, with an overall glucuronidation of 98\%. Total isoflavones showed a breast adipose/glandular tissue distribution of 40: 60, and their mean (+/- SEM) derived 17 beta-estradiol equivalents toward estrogen receptor beta were 21 +/- 4-fold and 40 +/- 10-fold higher than the 17 beta-estradiol concentrations in adipose (0.283 +/- 6 0.089 pmol/g, P {\textlangle} 0.001) and glandular (0.246 +/- 0.091 pmol/g, P = 0.001) fractions, respectively.
Conclusion: After intake of soy milk and soy supplements, isoflavones reach exposure levels in breast tissue at which potential health effects may occur.},
  author       = {Bolca, Selin and Urpi-Sarda, Mireia and Blondeel, Phillip and Roche, Nathalie and Vanhaecke, Lynn and Possemiers, Sam and Al-Maharik, Nawaf and Botting, Nigel and De Keukeleire, Denis and Bracke, Marc and Heyerick, Arne and Manach, Claudine and Depypere, Herman},
  issn         = {0002-9165},
  journal      = {AMERICAN JOURNAL OF CLINICAL NUTRITION},
  keyword      = {MAMMARY-GLAND,MCF-7 TUMORS,STIMULATE GROWTH,AROMATASE ACTIVITIES,IN-VITRO,POSTMENOPAUSAL WOMEN,ESTROGEN-RECEPTOR-ALPHA,CANCER TUMORS,HUMAN URINE,PHYTOESTROGENS},
  language     = {eng},
  number       = {4},
  pages        = {976--984},
  title        = {Disposition of soy isoflavones in normal human breast tissue},
  url          = {http://dx.doi.org/10.3945/ajcn.2009.28854},
  volume       = {91},
  year         = {2010},
}

Chicago
Bolca, Selin, Mireia Urpi-Sarda, Phillip Blondeel, NATHALIE ROCHE, Lynn Vanhaecke, Sam Possemiers, Nawaf Al-Maharik, et al. 2010. “Disposition of Soy Isoflavones in Normal Human Breast Tissue.” American Journal of Clinical Nutrition 91 (4): 976–984.
APA
Bolca, S., Urpi-Sarda, M., Blondeel, P., ROCHE, N., Vanhaecke, L., Possemiers, S., Al-Maharik, N., et al. (2010). Disposition of soy isoflavones in normal human breast tissue. AMERICAN JOURNAL OF CLINICAL NUTRITION, 91(4), 976–984.
Vancouver
1.
Bolca S, Urpi-Sarda M, Blondeel P, ROCHE N, Vanhaecke L, Possemiers S, et al. Disposition of soy isoflavones in normal human breast tissue. AMERICAN JOURNAL OF CLINICAL NUTRITION. 2010;91(4):976–84.
MLA
Bolca, Selin, Mireia Urpi-Sarda, Phillip Blondeel, et al. “Disposition of Soy Isoflavones in Normal Human Breast Tissue.” AMERICAN JOURNAL OF CLINICAL NUTRITION 91.4 (2010): 976–984. Print.