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Trafficking to the outer polar domain defines the root-soil interface

Lukasz Langowski (UGent) , Kamil Ruzicka (UGent) , Satoshi Naramoto (UGent) , Jürgen Kleine-Vehn (UGent) and Jiri Friml (UGent)
(2010) CURRENT BIOLOGY. 20(10). p.904-908
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Abstract
In animals, the interface between organism and environment is constituted by the epithelium [1]. In plants, the exchange of nutrients and signals between root and soil is crucial for their survival, but the cellular mechanisms underlying the epithelium-like function and specific localization of proteins to the root surface have not been identified [2]. Here we analyze the mechanism of polar delivery to the root-soil interface of the proteins BOR4, ABCG37, and PEN3, which transport nutrients [2], transport plant hormones, and are required for pathogen defense [3], respectively. The simultaneous visualization of these proteins and the apical and basal cargos in a single cell demonstrates that the outermost cell side represents an additional polar domain. Delivery to this outer polar domain depends on ARF GEF [4] and actin [5-8] function but does not require known molecular components of the apical or basal targeting. The outer polar delivery is, in contrast to known basal and apical cargos [9, 10], mediated by the polar secretion. Our findings show that the outermost cell membranes of roots define an additional polar domain in plant cells along with a specific, previously uncharacterized, polar targeting mechanism that is important for defining the functional, epithelium-like root-soil interface.
Keywords
RESISTANCE, ACTIN, AUXIN TRANSPORT, BINDING CASSETTE TRANSPORTER, ARABIDOPSIS-THALIANA, PLANT DEVELOPMENT, LOCALIZATION, GROWTH, EFFLUX, DISTINCT

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Citation

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Chicago
Langowski, Lukasz, Kamil Ruzicka, Satoshi Naramoto, Jürgen Kleine-Vehn, and Jiri Friml. 2010. “Trafficking to the Outer Polar Domain Defines the Root-soil Interface.” Current Biology 20 (10): 904–908.
APA
Langowski, L., Ruzicka, K., Naramoto, S., Kleine-Vehn, J., & Friml, J. (2010). Trafficking to the outer polar domain defines the root-soil interface. CURRENT BIOLOGY, 20(10), 904–908.
Vancouver
1.
Langowski L, Ruzicka K, Naramoto S, Kleine-Vehn J, Friml J. Trafficking to the outer polar domain defines the root-soil interface. CURRENT BIOLOGY. 2010;20(10):904–8.
MLA
Langowski, Lukasz, Kamil Ruzicka, Satoshi Naramoto, et al. “Trafficking to the Outer Polar Domain Defines the Root-soil Interface.” CURRENT BIOLOGY 20.10 (2010): 904–908. Print.
@article{980228,
  abstract     = {In animals, the interface between organism and environment is constituted by the epithelium [1]. In plants, the exchange of nutrients and signals between root and soil is crucial for their survival, but the cellular mechanisms underlying the epithelium-like function and specific localization of proteins to the root surface have not been identified [2]. Here we analyze the mechanism of polar delivery to the root-soil interface of the proteins BOR4, ABCG37, and PEN3, which transport nutrients [2], transport plant hormones, and are required for pathogen defense [3], respectively. The simultaneous visualization of these proteins and the apical and basal cargos in a single cell demonstrates that the outermost cell side represents an additional polar domain. Delivery to this outer polar domain depends on ARF GEF [4] and actin [5-8] function but does not require known molecular components of the apical or basal targeting. The outer polar delivery is, in contrast to known basal and apical cargos [9, 10], mediated by the polar secretion. Our findings show that the outermost cell membranes of roots define an additional polar domain in plant cells along with a specific, previously uncharacterized, polar targeting mechanism that is important for defining the functional, epithelium-like root-soil interface.},
  author       = {Langowski, Lukasz and Ruzicka, Kamil and Naramoto, Satoshi and Kleine-Vehn, J{\"u}rgen and Friml, Jiri},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keyword      = {RESISTANCE,ACTIN,AUXIN TRANSPORT,BINDING CASSETTE TRANSPORTER,ARABIDOPSIS-THALIANA,PLANT DEVELOPMENT,LOCALIZATION,GROWTH,EFFLUX,DISTINCT},
  language     = {eng},
  number       = {10},
  pages        = {904--908},
  title        = {Trafficking to the outer polar domain defines the root-soil interface},
  url          = {http://dx.doi.org/10.1016/j.cub.2010.03.059},
  volume       = {20},
  year         = {2010},
}

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