Micro-CT of corrosion casts for use in the computer-aided design of microvasculature
(2009) TISSUE ENGINEERING PART C-METHODS. 15(4). p.729-738- abstract
- Two-dimensional micro-computed tomography (micro-CT) slices can be reconstructed into three-dimensional (3D) models that demonstrate capillary beds. This study focused on the acquisition of data necessary to create scaffolding that directly mimics the unique structural patterns of a microvascular tree system. The Microfil (TM) vascular contrasting method was compared to the Baston's methylmethacrylate corrosion casting (BMCC) method to determine which provided the most accurate and high-resolution results for 3D micro-CT reconstruction derived from the two-dimensional micro-CT slices of the capillary beds. It was determined that the BMCC, a method traditionally used in the scanning electron microscopic analysis of the microvasculature, was the best method for representing capillary lumina for micro-CT scanning. The removal of tissues from the BMCC cast resulted in samples that eliminated background material, thus increasing the X-ray contrast levels of the CT images. This provided for a more complete and more distinguishable high-resolution image of the represented capillary lumina. Images created with this BMCC method were reconstructed in a stereolithography file format as 3D mesh structure for later importing into computer-aided design (CAD) software. The resulting Bio-CAD, then, can be used to guide the more accurate fabrication of the microvascular scaffolding and then serve as the framework for tissue engineering of microvascular structures. Results from this study clearly indicated that the BMCC method is superior to the Microfil method for accurate and complete high-resolution imaging of capillary beds.
Please use this url to cite or link to this publication:
http://hdl.handle.net/1854/LU-978237
- author
- William Lafayette Mondy, Don Cameron, Jean-Pierre Timmermans, Nora De Clerck, Alexander Sasov, Christophe Casteleyn UGent and Les A Piegl
- organization
- year
- 2009
- type
- journalArticle (original)
- publication status
- published
- subject
- keyword
- GOLD NANOPARTICLES, POOL CONTRAST AGENT, IN-VIVO, BIO-CAD, TOMOGRAPHY, TISSUE, VISUALIZATION, LUNG, QUANTIFICATION, ANGIOGRAPHY
- journal title
- TISSUE ENGINEERING PART C-METHODS
- Tissue Eng. Part C-Methods
- volume
- 15
- issue
- 4
- pages
- 729 - 738
- Web of Science type
- Article
- Web of Science id
- 000272609100020
- ISSN
- 1937-3384
- DOI
- 10.1089/ten.tec.2008.0583
- language
- English
- UGent publication?
- yes
- classification
- A1
- copyright statement
- I have transferred the copyright for this publication to the publisher
- id
- 978237
- handle
- http://hdl.handle.net/1854/LU-978237
- date created
- 2010-06-14 15:37:04
- date last changed
- 2016-12-19 15:46:23
@article{978237, abstract = {Two-dimensional micro-computed tomography (micro-CT) slices can be reconstructed into three-dimensional (3D) models that demonstrate capillary beds. This study focused on the acquisition of data necessary to create scaffolding that directly mimics the unique structural patterns of a microvascular tree system. The Microfil (TM) vascular contrasting method was compared to the Baston's methylmethacrylate corrosion casting (BMCC) method to determine which provided the most accurate and high-resolution results for 3D micro-CT reconstruction derived from the two-dimensional micro-CT slices of the capillary beds. It was determined that the BMCC, a method traditionally used in the scanning electron microscopic analysis of the microvasculature, was the best method for representing capillary lumina for micro-CT scanning. The removal of tissues from the BMCC cast resulted in samples that eliminated background material, thus increasing the X-ray contrast levels of the CT images. This provided for a more complete and more distinguishable high-resolution image of the represented capillary lumina. Images created with this BMCC method were reconstructed in a stereolithography file format as 3D mesh structure for later importing into computer-aided design (CAD) software. The resulting Bio-CAD, then, can be used to guide the more accurate fabrication of the microvascular scaffolding and then serve as the framework for tissue engineering of microvascular structures. Results from this study clearly indicated that the BMCC method is superior to the Microfil method for accurate and complete high-resolution imaging of capillary beds.}, author = {Mondy, William Lafayette and Cameron, Don and Timmermans, Jean-Pierre and De Clerck, Nora and Sasov, Alexander and Casteleyn, Christophe and Piegl, Les A}, issn = {1937-3384}, journal = {TISSUE ENGINEERING PART C-METHODS}, keyword = {GOLD NANOPARTICLES,POOL CONTRAST AGENT,IN-VIVO,BIO-CAD,TOMOGRAPHY,TISSUE,VISUALIZATION,LUNG,QUANTIFICATION,ANGIOGRAPHY}, language = {eng}, number = {4}, pages = {729--738}, title = {Micro-CT of corrosion casts for use in the computer-aided design of microvasculature}, url = {http://dx.doi.org/10.1089/ten.tec.2008.0583}, volume = {15}, year = {2009}, }
- Chicago
- Mondy, William Lafayette, Don Cameron, Jean-Pierre Timmermans, Nora De Clerck, Alexander Sasov, Christophe Casteleyn, and Les A Piegl. 2009. “Micro-CT of Corrosion Casts for Use in the Computer-aided Design of Microvasculature.” Tissue Engineering Part C-methods 15 (4): 729–738.
- APA
- Mondy, W. L., Cameron, D., Timmermans, J.-P., De Clerck, N., Sasov, A., Casteleyn, C., & Piegl, L. A. (2009). Micro-CT of corrosion casts for use in the computer-aided design of microvasculature. TISSUE ENGINEERING PART C-METHODS, 15(4), 729–738.
- Vancouver
- 1.Mondy WL, Cameron D, Timmermans J-P, De Clerck N, Sasov A, Casteleyn C, et al. Micro-CT of corrosion casts for use in the computer-aided design of microvasculature. TISSUE ENGINEERING PART C-METHODS. 2009;15(4):729–38.
- MLA
- Mondy, William Lafayette, Don Cameron, Jean-Pierre Timmermans, et al. “Micro-CT of Corrosion Casts for Use in the Computer-aided Design of Microvasculature.” TISSUE ENGINEERING PART C-METHODS 15.4 (2009): 729–738. Print.