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An immunochemical test for rapid screening of zearalenone and T-2 toxin

Evgenia Yu Basova, Irina Yu Goryacheva, Tatiana Yu Rusanova, Natalia A Burmistrova, Richard Dietrich, Erwin Martlbauer, Christel Detavernier UGent, Carlos Van Peteghem UGent and Sarah De Saeger UGent (2010) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 397(1). p.55-62
abstract
An immunochemically based test for noninstrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 mu g/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MYCOTOXINS, OCHRATOXIN-A, SAMPLES, DEOXYNIVALENOL, ENZYME-IMMUNOASSAY, NONINSTRUMENTAL DETECTION, TANDEM MASS-SPECTROMETRY, AFLATOXIN B-1, ASSAY COLUMN, TRICHOTHECENES, T-2 toxin, Feed, Mycotoxin, Zearalenone, Fusarium toxin, Visual detection, Non-instrumental test
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
397
issue
1
pages
8 pages
Web of Science type
Article
Web of Science id
000276738800009
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.841 (2010)
JCR rank
9/71 (2010)
JCR quartile
1 (2010)
ISSN
1618-2642
DOI
10.1007/s00216-009-3328-1
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
977182
handle
http://hdl.handle.net/1854/LU-977182
date created
2010-06-11 13:41:42
date last changed
2010-06-24 11:45:49
@article{977182,
  abstract     = {An immunochemically based test for noninstrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 mu g/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.},
  author       = {Basova, Evgenia Yu and Goryacheva, Irina Yu and Rusanova, Tatiana Yu and Burmistrova, Natalia A and Dietrich, Richard and Martlbauer, Erwin and Detavernier, Christel and Van Peteghem, Carlos and De Saeger, Sarah},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {MYCOTOXINS,OCHRATOXIN-A,SAMPLES,DEOXYNIVALENOL,ENZYME-IMMUNOASSAY,NONINSTRUMENTAL DETECTION,TANDEM MASS-SPECTROMETRY,AFLATOXIN B-1,ASSAY COLUMN,TRICHOTHECENES,T-2 toxin,Feed,Mycotoxin,Zearalenone,Fusarium toxin,Visual detection,Non-instrumental test},
  language     = {eng},
  number       = {1},
  pages        = {55--62},
  title        = {An immunochemical test for rapid screening of zearalenone and T-2 toxin},
  url          = {http://dx.doi.org/10.1007/s00216-009-3328-1},
  volume       = {397},
  year         = {2010},
}

Chicago
Basova, Evgenia Yu, Irina Yu Goryacheva, Tatiana Yu Rusanova, Natalia A Burmistrova, Richard Dietrich, Erwin Martlbauer, Christel Detavernier, Carlos Van Peteghem, and Sarah De Saeger. 2010. “An Immunochemical Test for Rapid Screening of Zearalenone and T-2 Toxin.” Analytical and Bioanalytical Chemistry 397 (1): 55–62.
APA
Basova, E. Y., Goryacheva, I. Y., Rusanova, T. Y., Burmistrova, N. A., Dietrich, R., Martlbauer, E., Detavernier, C., et al. (2010). An immunochemical test for rapid screening of zearalenone and T-2 toxin. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 397(1), 55–62.
Vancouver
1.
Basova EY, Goryacheva IY, Rusanova TY, Burmistrova NA, Dietrich R, Martlbauer E, et al. An immunochemical test for rapid screening of zearalenone and T-2 toxin. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2010;397(1):55–62.
MLA
Basova, Evgenia Yu, Irina Yu Goryacheva, Tatiana Yu Rusanova, et al. “An Immunochemical Test for Rapid Screening of Zearalenone and T-2 Toxin.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 397.1 (2010): 55–62. Print.