Advanced search
1 file | 1.04 MB

Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum

Author
Organization
Abstract
Background: Fusarium head blight is a very important disease of small grain cereals with F. graminearum as one of the most important causal agents. It not only causes reduction in yield and quality but from a human and animal healthcare point of view, it produces mycotoxins such as deoxynivalenol (DON) which can accumulate to toxic levels. Little is known about external triggers influencing DON production. Results: In the present work, a combined in vivo/ in vitro approach was used to test the effect of sub lethal fungicide treatments on DON production. Using a dilution series of prothioconazole, azoxystrobin and prothioconazole + fluoxastrobin, we demonstrated that sub lethal doses of prothioconazole coincide with an increase in DON production 48 h after fungicide treatment. In an artificial infection trial using wheat plants, the in vitro results of increased DON levels upon sub lethal prothioconazole application were confirmed illustrating the significance of these results from a practical point of view. In addition, further in vitro experiments revealed a timely hyperinduction of H2O2 production as fast as 4 h after amending cultures with prothioconazole. When applying H2O2 directly to germinating conidia, a similar induction of DON-production by F. graminearum was observed. The effect of sub lethal prothioconazole concentrations on DON production completely disappeared when applying catalase together with the fungicide. Conclusions: These cumulative results suggest that H2O2 induced by sub lethal doses of the triazole fungicide prothioconazole acts as a trigger of DON biosynthesis. In a broader framework, this work clearly shows that DON production by the plant pathogen F. graminearum is the result of the interaction of fungal genomics and external environmental triggers.
Keywords
SACCHAROMYCES-CEREVISIAE, HEAD BLIGHT, OXIDATIVE STRESS, MYCOTOXIN DEOXYNIVALENOL, PLASMA-MEMBRANE, ACTIVE OXYGEN, WHEAT SPIKES, CYTOCHROME-B, LIQUID CULTURES, REAL-TIME PCR

Downloads

  • 1471-2180-10-112.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.04 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Audenaert, Kris, Elien Callewaert, Monica Höfte, Sarah De Saeger, and Geert Haesaert. 2010. “Hydrogen Peroxide Induced by the Fungicide Prothioconazole Triggers Deoxynivalenol (DON) Production by Fusarium Graminearum.” Bmc Microbiology 10.
APA
Audenaert, Kris, Callewaert, E., Höfte, M., De Saeger, S., & Haesaert, G. (2010). Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum. BMC MICROBIOLOGY, 10.
Vancouver
1.
Audenaert K, Callewaert E, Höfte M, De Saeger S, Haesaert G. Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum. BMC MICROBIOLOGY. 2010;10.
MLA
Audenaert, Kris, Elien Callewaert, Monica Höfte, et al. “Hydrogen Peroxide Induced by the Fungicide Prothioconazole Triggers Deoxynivalenol (DON) Production by Fusarium Graminearum.” BMC MICROBIOLOGY 10 (2010): n. pag. Print.
@article{977167,
  abstract     = {Background: Fusarium head blight is a very important disease of small grain cereals with F. graminearum as one of the most important causal agents. It not only causes reduction in yield and quality but from a human and animal healthcare point of view, it produces mycotoxins such as deoxynivalenol (DON) which can accumulate to toxic levels. Little is known about external triggers influencing DON production.
Results: In the present work, a combined in vivo/ in vitro approach was used to test the effect of sub lethal fungicide treatments on DON production. Using a dilution series of prothioconazole, azoxystrobin and prothioconazole + fluoxastrobin, we demonstrated that sub lethal doses of prothioconazole coincide with an increase in DON production 48 h after fungicide treatment. In an artificial infection trial using wheat plants, the in vitro results of increased DON levels upon sub lethal prothioconazole application were confirmed illustrating the significance of these results from a practical point of view. In addition, further in vitro experiments revealed a timely hyperinduction of H2O2 production as fast as 4 h after amending cultures with prothioconazole. When applying H2O2 directly to germinating conidia, a similar induction of DON-production by F. graminearum was observed. The effect of sub lethal prothioconazole concentrations on DON production completely disappeared when applying catalase together with the fungicide.
Conclusions: These cumulative results suggest that H2O2 induced by sub lethal doses of the triazole fungicide prothioconazole acts as a trigger of DON biosynthesis. In a broader framework, this work clearly shows that DON production by the plant pathogen F. graminearum is the result of the interaction of fungal genomics and external environmental triggers.},
  articleno    = {112},
  author       = {Audenaert, Kris and Callewaert, Elien and H{\"o}fte, Monica and De Saeger, Sarah and Haesaert, Geert},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {SACCHAROMYCES-CEREVISIAE,HEAD BLIGHT,OXIDATIVE STRESS,MYCOTOXIN DEOXYNIVALENOL,PLASMA-MEMBRANE,ACTIVE OXYGEN,WHEAT SPIKES,CYTOCHROME-B,LIQUID CULTURES,REAL-TIME PCR},
  language     = {eng},
  pages        = {14},
  title        = {Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum},
  url          = {http://dx.doi.org/10.1186/1471-2180-10-112},
  volume       = {10},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: