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Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum

Kris Audenaert UGent, Elien Callewaert, Monica Höfte UGent, Sarah De Saeger UGent and Geert Haesaert UGent (2010) BMC MICROBIOLOGY. 10.
abstract
Background: Fusarium head blight is a very important disease of small grain cereals with F. graminearum as one of the most important causal agents. It not only causes reduction in yield and quality but from a human and animal healthcare point of view, it produces mycotoxins such as deoxynivalenol (DON) which can accumulate to toxic levels. Little is known about external triggers influencing DON production. Results: In the present work, a combined in vivo/ in vitro approach was used to test the effect of sub lethal fungicide treatments on DON production. Using a dilution series of prothioconazole, azoxystrobin and prothioconazole + fluoxastrobin, we demonstrated that sub lethal doses of prothioconazole coincide with an increase in DON production 48 h after fungicide treatment. In an artificial infection trial using wheat plants, the in vitro results of increased DON levels upon sub lethal prothioconazole application were confirmed illustrating the significance of these results from a practical point of view. In addition, further in vitro experiments revealed a timely hyperinduction of H2O2 production as fast as 4 h after amending cultures with prothioconazole. When applying H2O2 directly to germinating conidia, a similar induction of DON-production by F. graminearum was observed. The effect of sub lethal prothioconazole concentrations on DON production completely disappeared when applying catalase together with the fungicide. Conclusions: These cumulative results suggest that H2O2 induced by sub lethal doses of the triazole fungicide prothioconazole acts as a trigger of DON biosynthesis. In a broader framework, this work clearly shows that DON production by the plant pathogen F. graminearum is the result of the interaction of fungal genomics and external environmental triggers.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SACCHAROMYCES-CEREVISIAE, HEAD BLIGHT, OXIDATIVE STRESS, MYCOTOXIN DEOXYNIVALENOL, PLASMA-MEMBRANE, ACTIVE OXYGEN, WHEAT SPIKES, CYTOCHROME-B, LIQUID CULTURES, REAL-TIME PCR
journal title
BMC MICROBIOLOGY
BMC Microbiol.
volume
10
article_number
112
pages
14 pages
Web of Science type
Article
Web of Science id
000277076000001
JCR category
MICROBIOLOGY
JCR impact factor
2.96 (2010)
JCR rank
38/103 (2010)
JCR quartile
2 (2010)
ISSN
1471-2180
DOI
10.1186/1471-2180-10-112
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
977167
handle
http://hdl.handle.net/1854/LU-977167
date created
2010-06-11 13:41:42
date last changed
2014-02-27 09:56:59
@article{977167,
  abstract     = {Background: Fusarium head blight is a very important disease of small grain cereals with F. graminearum as one of the most important causal agents. It not only causes reduction in yield and quality but from a human and animal healthcare point of view, it produces mycotoxins such as deoxynivalenol (DON) which can accumulate to toxic levels. Little is known about external triggers influencing DON production.
Results: In the present work, a combined in vivo/ in vitro approach was used to test the effect of sub lethal fungicide treatments on DON production. Using a dilution series of prothioconazole, azoxystrobin and prothioconazole + fluoxastrobin, we demonstrated that sub lethal doses of prothioconazole coincide with an increase in DON production 48 h after fungicide treatment. In an artificial infection trial using wheat plants, the in vitro results of increased DON levels upon sub lethal prothioconazole application were confirmed illustrating the significance of these results from a practical point of view. In addition, further in vitro experiments revealed a timely hyperinduction of H2O2 production as fast as 4 h after amending cultures with prothioconazole. When applying H2O2 directly to germinating conidia, a similar induction of DON-production by F. graminearum was observed. The effect of sub lethal prothioconazole concentrations on DON production completely disappeared when applying catalase together with the fungicide.
Conclusions: These cumulative results suggest that H2O2 induced by sub lethal doses of the triazole fungicide prothioconazole acts as a trigger of DON biosynthesis. In a broader framework, this work clearly shows that DON production by the plant pathogen F. graminearum is the result of the interaction of fungal genomics and external environmental triggers.},
  articleno    = {112},
  author       = {Audenaert, Kris and Callewaert, Elien and H{\"o}fte, Monica and De Saeger, Sarah and Haesaert, Geert},
  issn         = {1471-2180},
  journal      = {BMC MICROBIOLOGY},
  keyword      = {SACCHAROMYCES-CEREVISIAE,HEAD BLIGHT,OXIDATIVE STRESS,MYCOTOXIN DEOXYNIVALENOL,PLASMA-MEMBRANE,ACTIVE OXYGEN,WHEAT SPIKES,CYTOCHROME-B,LIQUID CULTURES,REAL-TIME PCR},
  language     = {eng},
  pages        = {14},
  title        = {Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum},
  url          = {http://dx.doi.org/10.1186/1471-2180-10-112},
  volume       = {10},
  year         = {2010},
}

Chicago
Audenaert, Kris, Elien Callewaert, Monica Höfte, Sarah De Saeger, and Geert Haesaert. 2010. “Hydrogen Peroxide Induced by the Fungicide Prothioconazole Triggers Deoxynivalenol (DON) Production by Fusarium Graminearum.” Bmc Microbiology 10.
APA
Audenaert, Kris, Callewaert, E., Höfte, M., De Saeger, S., & Haesaert, G. (2010). Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum. BMC MICROBIOLOGY, 10.
Vancouver
1.
Audenaert K, Callewaert E, Höfte M, De Saeger S, Haesaert G. Hydrogen peroxide induced by the fungicide prothioconazole triggers deoxynivalenol (DON) production by Fusarium graminearum. BMC MICROBIOLOGY. 2010;10.
MLA
Audenaert, Kris, Elien Callewaert, Monica Höfte, et al. “Hydrogen Peroxide Induced by the Fungicide Prothioconazole Triggers Deoxynivalenol (DON) Production by Fusarium Graminearum.” BMC MICROBIOLOGY 10 (2010): n. pag. Print.