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Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle

Reza Soleimani UGent, Elke Heytens UGent, Rudy Van den Broecke UGent, ISABELLE ROTTIERS UGent, MARC DHONT UGent, Claude Cuvelier UGent and Petra De Sutter UGent (2010) HUMAN REPRODUCTION. 25(6). p.1458-1470
abstract
Background: Ovarian tissue (OT) cryopreservation and transplantation are options for fertility preservation in young female cancer patients. Methods: We investigated xenotransplantation of human OT into back muscle (B) of severe combined immunodeficiency mice. OT follicle content was evaluated by stereomicroscopy and pre-transplantation. Xenograft survival, follicular development (with/without FSH administration), apoptosis and vascularization were compared in B-versus K-site (under the kidney capsule) several times after grafting using histology, immunohistochemistry and magnetic resonance imaging. In vitro maturation (IVM) was also performed. Results: Anastomoses which developed from existing human and invading murine vessels were seen in OT at both sites, but angiogenesis was more prominent at the B-than K-site (P < 0.001). Vascularization and follicle size were correlated in the B-group (Spearman's coefficient 0.73; P < 0.001). FSH increased early (8 days) micro-vessel formation in B but not in K grafts (P < 0.0001, versus no FSH). B-site grafts showed a better histological morphology and survival (P = 0.0084), formation of larger antral follicles (P = 0.005), more metaphase-II (MII) oocytes, growing follicles (P = 0.028) and slightly fewer apoptotic follicles than K grafts. One MI oocyte from B underwent IVM and reached MII stage next day. Conclusions: To our knowledge, this is the first report of MII and IVM-MII oocytes obtained from B xenografts. We report the largest oval-shaped antral follicles containing an MII oocyte obtained after OT xenotransplantation to date. Xenografting in the mouse B should be further explored as a method for human OT transplantation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ANTRAL FOLLICLES, SCID MICE, PRIMORDIAL FOLLICLES, CHILDHOOD-CANCER SURVIVOR, FOLLICLE-STIMULATING-HORMONE, IMMUNODEFICIENT MICE, CORTICAL STRIPS, OOCYTE MATURATION, PRELIMINARY EXPERIENCE, HETEROTOPIC TRANSPLANTATION
journal title
HUMAN REPRODUCTION
Hum. Reprod.
volume
25
issue
6
pages
13 pages
publisher
Oxford University Press
place of publication
OXFORD
Web of Science type
Article
Web of Science id
000277988200016
JCR category
OBSTETRICS & GYNECOLOGY
JCR impact factor
4.357 (2010)
JCR rank
3/75 (2010)
JCR quartile
1 (2010)
ISSN
0268-1161
DOI
10.1093/humrep/deq055
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
976908
handle
http://hdl.handle.net/1854/LU-976908
date created
2010-06-11 11:00:11
date last changed
2010-06-23 13:57:08
@article{976908,
  abstract     = {Background: Ovarian tissue (OT) cryopreservation and transplantation are options for fertility preservation in young female cancer patients.
Methods: We investigated xenotransplantation of human OT into back muscle (B) of severe combined immunodeficiency mice. OT follicle content was evaluated by stereomicroscopy and pre-transplantation. Xenograft survival, follicular development (with/without FSH administration), apoptosis and vascularization were compared in B-versus K-site (under the kidney capsule) several times after grafting using histology, immunohistochemistry and magnetic resonance imaging. In vitro maturation (IVM) was also performed.

Results: Anastomoses which developed from existing human and invading murine vessels were seen in OT at both sites, but angiogenesis was more prominent at the B-than K-site (P {\textlangle} 0.001). Vascularization and follicle size were correlated in the B-group (Spearman's coefficient 0.73; P {\textlangle} 0.001). FSH increased early (8 days) micro-vessel formation in B but not in K grafts (P {\textlangle} 0.0001, versus no FSH). B-site grafts showed a better histological morphology and survival (P = 0.0084), formation of larger antral follicles (P = 0.005), more metaphase-II (MII) oocytes, growing follicles (P = 0.028) and slightly fewer apoptotic follicles than K grafts. One MI oocyte from B underwent IVM and reached MII stage next day.

Conclusions: To our knowledge, this is the first report of MII and IVM-MII oocytes obtained from B xenografts. We report the largest oval-shaped antral follicles containing an MII oocyte obtained after OT xenotransplantation to date. Xenografting in the mouse B should be further explored as a method for human OT transplantation.},
  author       = {Soleimani, Reza and Heytens, Elke and Van den Broecke, Rudy and ROTTIERS, ISABELLE and DHONT, MARC and Cuvelier, Claude and De Sutter, Petra},
  issn         = {0268-1161},
  journal      = {HUMAN REPRODUCTION},
  keyword      = {ANTRAL FOLLICLES,SCID MICE,PRIMORDIAL FOLLICLES,CHILDHOOD-CANCER SURVIVOR,FOLLICLE-STIMULATING-HORMONE,IMMUNODEFICIENT MICE,CORTICAL STRIPS,OOCYTE MATURATION,PRELIMINARY EXPERIENCE,HETEROTOPIC TRANSPLANTATION},
  language     = {eng},
  number       = {6},
  pages        = {1458--1470},
  publisher    = {Oxford University Press},
  title        = {Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle},
  url          = {http://dx.doi.org/10.1093/humrep/deq055},
  volume       = {25},
  year         = {2010},
}

Chicago
Soleimani, Reza, Elke Heytens, Rudy Van den Broecke, ISABELLE ROTTIERS, MARC DHONT, Claude Cuvelier, and Petra De Sutter. 2010. “Xenotransplantation of Cryopreserved Human Ovarian Tissue into Murine Back Muscle.” Human Reproduction 25 (6): 1458–1470.
APA
Soleimani, R., Heytens, E., Van den Broecke, R., ROTTIERS, I., DHONT, M., Cuvelier, C., & De Sutter, P. (2010). Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle. HUMAN REPRODUCTION, 25(6), 1458–1470.
Vancouver
1.
Soleimani R, Heytens E, Van den Broecke R, ROTTIERS I, DHONT M, Cuvelier C, et al. Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle. HUMAN REPRODUCTION. OXFORD: Oxford University Press; 2010;25(6):1458–70.
MLA
Soleimani, Reza, Elke Heytens, Rudy Van den Broecke, et al. “Xenotransplantation of Cryopreserved Human Ovarian Tissue into Murine Back Muscle.” HUMAN REPRODUCTION 25.6 (2010): 1458–1470. Print.