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Identification of the nasal mucosa as a new target for leptin action

(2010) HISTOPATHOLOGY. 56(6). p.789-798
Author
Organization
Abstract
Aims: Leptin is a key molecule in the regulation of energy homeostasis. Furthermore, several studies indicate that leptin also plays a pivotal role in immune and inflammatory responses. The aim of this study was to examine systemic and local nasal leptin and leptin receptor expression in patients with nasal polyposis and healthy controls. Methods and results: Serum leptin and soluble leptin receptor levels were examined by enzyme-linked immunosorbent assay (ELISA). The presence of leptin and leptin receptor mRNA was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), and tissue leptin and leptin receptor protein expression was analysed by immunohistochemistry and ELISA. Serum levels of biologically active leptin were significantly elevated in patients with nasal polyps compared with control subjects. These serum leptin levels were strongly correlated with the levels found in tissue in both study groups, although leptin was not significantly elevated in nasal polyp tissue. Using RT-PCR, we showed that both leptin and its receptors were produced in nasal mucosa. Finally, immunohistochemistry showed that leptin and leptin receptor protein were expressed in several cells of the normal and inflamed nasal mucosa. Conclusions: Leptin receptors and their biological ligand leptin are expressed in the nasal mucosa, suggesting a possible role in upper airway immunology.
Keywords
leptin, nasal polyps, leptin receptor

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Chicago
Taildeman, Jasmien, Pieter Demetter, ISABELLE ROTTIERS, Gabriële Holtappels, Claus Bachert, Claude Cuvelier, and Claudina Perez Novo. 2010. “Identification of the Nasal Mucosa as a New Target for Leptin Action.” Histopathology 56 (6): 789–798.
APA
Taildeman, J., Demetter, P., ROTTIERS, I., Holtappels, G., Bachert, C., Cuvelier, C., & Perez Novo, C. (2010). Identification of the nasal mucosa as a new target for leptin action. HISTOPATHOLOGY, 56(6), 789–798.
Vancouver
1.
Taildeman J, Demetter P, ROTTIERS I, Holtappels G, Bachert C, Cuvelier C, et al. Identification of the nasal mucosa as a new target for leptin action. HISTOPATHOLOGY. USA: WILEY-BLACKWELL PUBLISHING, INC, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA; 2010;56(6):789–98.
MLA
Taildeman, Jasmien, Pieter Demetter, ISABELLE ROTTIERS, et al. “Identification of the Nasal Mucosa as a New Target for Leptin Action.” HISTOPATHOLOGY 56.6 (2010): 789–798. Print.
@article{976813,
  abstract     = {Aims:
Leptin is a key molecule in the regulation of energy homeostasis. Furthermore, several studies indicate that leptin also plays a pivotal role in immune and inflammatory responses. The aim of this study was to examine systemic and local nasal leptin and leptin receptor expression in patients with nasal polyposis and healthy controls.

Methods and results:

Serum leptin and soluble leptin receptor levels were examined by enzyme-linked immunosorbent assay (ELISA). The presence of leptin and leptin receptor mRNA was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), and tissue leptin and leptin receptor protein expression was analysed by immunohistochemistry and ELISA.

Serum levels of biologically active leptin were significantly elevated in patients with nasal polyps compared with control subjects. These serum leptin levels were strongly correlated with the levels found in tissue in both study groups, although leptin was not significantly elevated in nasal polyp tissue. Using RT-PCR, we showed that both leptin and its receptors were produced in nasal mucosa. Finally, immunohistochemistry showed that leptin and leptin receptor protein were expressed in several cells of the normal and inflamed nasal mucosa.

Conclusions:

Leptin receptors and their biological ligand leptin are expressed in the nasal mucosa, suggesting a possible role in upper airway immunology.},
  author       = {Taildeman, Jasmien and Demetter, Pieter and Rottiers, Isabelle and Holtappels, Gabri{\"e}le and Bachert, Claus and Cuvelier, Claude and Perez Novo, Claudina},
  issn         = {0309-0167},
  journal      = {HISTOPATHOLOGY},
  keyword      = {leptin,nasal polyps,leptin receptor},
  language     = {eng},
  number       = {6},
  pages        = {789--798},
  publisher    = {WILEY-BLACKWELL PUBLISHING, INC, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA},
  title        = {Identification of the nasal mucosa as a new target for leptin action},
  url          = {http://dx.doi.org/10.1111/j.1365-2559.2010.03552.x},
  volume       = {56},
  year         = {2010},
}

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