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Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species

Gu Qun UGent, Frank David UGent, Lucie Jorge, Frederic Lynen UGent, Koen Sandra, Klaus Rumpel, Guowang Xu and Patrick Sandra UGent (2010) Capillary Chromatography, 34th International symposium, Abstracts. p.175-175
abstract
One of the most critical parameters in GC-MS application to metabolimic studies is the sample preparation. Often a combination of oximation and silylation is used and this method is applicable to acids (e.g. fatty acids), sterols, amines, amino acids, sugars, etc. Automation of the derivatisation process enhances repeatability and the time between sample preparation and analysis can be kept short and constant. For analysis, retention time locked (RTL) gas chromatography/mass spectrometry was used. For data analysis, AMDIS deconvolution followed by 2 multivariate statistical approaches were used and compared. Arabidopsis thaliana, a popular model organism in plant biology and genetics, was used for testing. The data analysis software allowed the detection of several features that were up- or downregulated in the different species.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
keyword
Fiehn library, amino acid, putrescine, Retention time locking (RTL), arabidopsis thaliana leaf, gas chromatography/mass spectrometry (GC/MS), metabolomics, multivariate statistical analyses (MVA)
in
Capillary Chromatography, 34th International symposium, Abstracts
pages
175 - 175
conference name
34th International symposium on Capillary Chromatography ; 7th GC×GC Symposium
conference location
Riva del Garda, Italy
conference start
2010-05-30
conference end
2010-06-04
language
English
UGent publication?
yes
classification
C3
copyright statement
I have retained and own the full copyright for this publication
id
975896
handle
http://hdl.handle.net/1854/LU-975896
date created
2010-06-09 17:06:34
date last changed
2010-07-13 14:29:42
@inproceedings{975896,
  abstract     = {One of the most critical parameters in GC-MS application to metabolimic studies is the sample preparation. Often a combination of oximation and silylation is used and this method is applicable to acids (e.g. fatty acids), sterols, amines, amino acids, sugars, etc. Automation of the derivatisation process enhances repeatability and the time between sample preparation and analysis can be kept short and constant. For analysis, retention time locked (RTL) gas chromatography/mass spectrometry was used. 
For data analysis, AMDIS deconvolution followed by 2 multivariate statistical approaches were used and compared. Arabidopsis thaliana, a popular model organism in plant biology and genetics, was used for testing. The data analysis software allowed the detection of several features that were up- or downregulated in the different species.},
  author       = {Qun, Gu and David, Frank and Jorge, Lucie and Lynen, Frederic and Sandra, Koen and Rumpel, Klaus and Xu, Guowang and Sandra, Patrick},
  booktitle    = {Capillary Chromatography, 34th International symposium, Abstracts},
  keyword      = {Fiehn library,amino acid,putrescine,Retention time locking (RTL),arabidopsis thaliana leaf,gas chromatography/mass spectrometry (GC/MS),metabolomics,multivariate statistical analyses (MVA)},
  language     = {eng},
  location     = {Riva del Garda, Italy},
  pages        = {175--175},
  title        = {Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species},
  year         = {2010},
}

Chicago
Qun, Gu, Frank David, Lucie Jorge, Frederic Lynen, Koen Sandra, Klaus Rumpel, Guowang Xu, and Patrick Sandra. 2010. “Evaluation of Automated Sample Preparation, Retention Time Locked GC-MS and Automated Data Analysis for the Metabolomic Study of Arabidopsis Species.” In Capillary Chromatography, 34th International Symposium, Abstracts, 175–175.
APA
Qun, G., David, F., Jorge, L., Lynen, F., Sandra, K., Rumpel, K., Xu, G., et al. (2010). Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species. Capillary Chromatography, 34th International symposium, Abstracts (pp. 175–175). Presented at the 34th International symposium on Capillary Chromatography ; 7th GC×GC Symposium.
Vancouver
1.
Qun G, David F, Jorge L, Lynen F, Sandra K, Rumpel K, et al. Evaluation of automated sample preparation, retention time locked GC-MS and automated data analysis for the metabolomic study of Arabidopsis species. Capillary Chromatography, 34th International symposium, Abstracts. 2010. p. 175–175.
MLA
Qun, Gu, Frank David, Lucie Jorge, et al. “Evaluation of Automated Sample Preparation, Retention Time Locked GC-MS and Automated Data Analysis for the Metabolomic Study of Arabidopsis Species.” Capillary Chromatography, 34th International Symposium, Abstracts. 2010. 175–175. Print.