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Analysis of bacterial fatty acids by flow modulated comprehensive two dimensional gas chromatography with parallel flame ionization detector/mass spectrometry

Gu Qun (UGent) , Frank David (UGent) , Frederic Lynen (UGent) , Klaus Rumpel, Guowang Xu and Patrick Sandra (UGent)
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Abstract
Comprehensive two-dimensional gas chromatography (GC×GC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GC×GC using a commercially available system was evaluated, and different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia by using parallel flame ionization detector (FID)/mass spectrometry (MS). The GC×GC plots obtained for a reference sample of bacterial fatty acid esters and a bacteria sample (S. maltophilia) were very similar to those obtained by using thermal modulated GC×GC. The parallel FID/MS set-up is useful since the MS allows identification and confirmation, while the FID allows comparison of the relative fatty acid composition with existing databases. The data show that flow modulated GC×GC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria.
Keywords
mass spectrometry, bacterial fatty acid methyl esters, GC×GC, parallel detection, flow modulation

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Citation

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Chicago
Qun, Gu, Frank David, Frederic Lynen, Klaus Rumpel, Guowang Xu, and Patrick Sandra. 2010. “Analysis of Bacterial Fatty Acids by Flow Modulated Comprehensive Two Dimensional Gas Chromatography with Parallel Flame Ionization Detector/mass Spectrometry.” In Capillary Chromatography, 34th International Symposium, Abstracts, 286–286.
APA
Qun, G., David, F., Lynen, F., Rumpel, K., Xu, G., & Sandra, P. (2010). Analysis of bacterial fatty acids by flow modulated comprehensive two dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. Capillary Chromatography, 34th International symposium, Abstracts (pp. 286–286). Presented at the 34th International symposium on Capillary Chromatography ; 7th GC×GC Symposium.
Vancouver
1.
Qun G, David F, Lynen F, Rumpel K, Xu G, Sandra P. Analysis of bacterial fatty acids by flow modulated comprehensive two dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. Capillary Chromatography, 34th International symposium, Abstracts. 2010. p. 286–286.
MLA
Qun, Gu, Frank David, Frederic Lynen, et al. “Analysis of Bacterial Fatty Acids by Flow Modulated Comprehensive Two Dimensional Gas Chromatography with Parallel Flame Ionization Detector/mass Spectrometry.” Capillary Chromatography, 34th International Symposium, Abstracts. 2010. 286–286. Print.
@inproceedings{975839,
  abstract     = {Comprehensive two-dimensional gas chromatography (GC{\texttimes}GC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GC{\texttimes}GC using a commercially available system was evaluated, and different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia by using parallel flame ionization detector (FID)/mass spectrometry (MS). The GC{\texttimes}GC plots obtained for a reference sample of bacterial fatty acid esters and a bacteria sample (S. maltophilia) were very similar to those obtained by using thermal modulated GC{\texttimes}GC.  The parallel FID/MS set-up is useful since the MS allows identification and confirmation, while the FID allows comparison of the relative fatty acid composition with existing databases. The data show that flow modulated GC{\texttimes}GC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria.},
  author       = {Qun, Gu and David, Frank and Lynen, Frederic and Rumpel, Klaus and Xu, Guowang and Sandra, Patrick},
  booktitle    = {Capillary Chromatography, 34th International symposium, Abstracts},
  keyword      = {mass spectrometry,bacterial fatty acid methyl esters,GC{\texttimes}GC,parallel detection,flow modulation},
  language     = {eng},
  location     = {Riva del Garda, Italy},
  pages        = {286--286},
  title        = {Analysis of bacterial fatty acids by flow modulated comprehensive two dimensional gas chromatography with parallel flame ionization detector/mass spectrometry},
  year         = {2010},
}