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Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively

Alexandr Nemec, Martin Musilek, Ondrej Sedo, Thierry De Baere UGent, Martina Maixnerova, Tanny JK van der Reijden, Zbynek Zdrahal, Mario Vaneechoutte UGent and Lenie Dijkshoorn (2010) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 60(4). p.896-903
abstract
Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize o-glucose and to grow at 38 C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46(T) =ATCC 11171(T) = CC U G 2491(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RECOGNITION, STRAINS, REPRODUCIBILITY, GENUS ACINETOBACTER, HUMAN CLINICAL SPECIMENS, IDENTIFICATION, PCR, BAUMANNII
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
60
issue
4
pages
8 pages
Web of Science type
Article
Web of Science id
000277733300033
JCR category
MICROBIOLOGY
JCR impact factor
1.93 (2010)
JCR rank
68/103 (2010)
JCR quartile
3 (2010)
ISSN
1466-5026
DOI
10.1099/ijs.0.013656-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
969801
handle
http://hdl.handle.net/1854/LU-969801
date created
2010-06-04 09:34:10
date last changed
2010-06-17 11:23:05
@article{969801,
  abstract     = {Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize o-glucose and to grow at 38 C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46(T) =ATCC 11171(T) = CC U G 2491(T)).},
  author       = {Nemec, Alexandr and Musilek, Martin and Sedo, Ondrej and De Baere, Thierry and Maixnerova, Martina and van der Reijden, Tanny JK and Zdrahal, Zbynek and Vaneechoutte, Mario and Dijkshoorn, Lenie},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {RECOGNITION,STRAINS,REPRODUCIBILITY,GENUS ACINETOBACTER,HUMAN CLINICAL SPECIMENS,IDENTIFICATION,PCR,BAUMANNII},
  language     = {eng},
  number       = {4},
  pages        = {896--903},
  title        = {Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively},
  url          = {http://dx.doi.org/10.1099/ijs.0.013656-0},
  volume       = {60},
  year         = {2010},
}

Chicago
Nemec, Alexandr, Martin Musilek, Ondrej Sedo, Thierry De Baere, Martina Maixnerova, Tanny JK van der Reijden, Zbynek Zdrahal, Mario Vaneechoutte, and Lenie Dijkshoorn. 2010. “Acinetobacter Bereziniae Sp Nov and Acinetobacter Guillouiae Sp Nov., to Accommodate Acinetobacter Genomic Species 10 and 11, Respectively.” International Journal of Systematic and Evolutionary Microbiology 60 (4): 896–903.
APA
Nemec, Alexandr, Musilek, M., Sedo, O., De Baere, T., Maixnerova, M., van der Reijden, T. J., Zdrahal, Z., et al. (2010). Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 60(4), 896–903.
Vancouver
1.
Nemec A, Musilek M, Sedo O, De Baere T, Maixnerova M, van der Reijden TJ, et al. Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2010;60(4):896–903.
MLA
Nemec, Alexandr, Martin Musilek, Ondrej Sedo, et al. “Acinetobacter Bereziniae Sp Nov and Acinetobacter Guillouiae Sp Nov., to Accommodate Acinetobacter Genomic Species 10 and 11, Respectively.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 60.4 (2010): 896–903. Print.