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Continuous CD27 triggering in vivo strongly reduces NK cell numbers

Veerle De Colvenaer, Sylvie Taveirne UGent, Jörg Hamann, Alex M de Bruin, Magda De Smedt, Tom Taghon UGent, Bart Vandekerckhove UGent, Jean Plum, René van Lier and Georges Leclercq UGent (2010) EUROPEAN JOURNAL OF IMMUNOLOGY. 40(4). p.1107-1117
abstract
NK cells are important mediators of the early defense. In mice, immature and mature NK (mNK) cells constitutively express the TNF receptor family member CD27; however, mNK cells eventually lose CD27 expression and become resting NK cells. Interaction of CD27 with its ligand, CD70, enhances proliferation and effector functions of NK cells. We used mice that constitutively express CD70 on B cells (CD70-Tg) to study the in vivo effects of continuous triggering of CD27 on NK cells. Continuous CD70-CD27 interaction resulted in strongly down-modulated CD27 expression on NK cells and gradually reduced absolute NK cell numbers. This reduction was most prominent in the mNK cell subpopulation and was at least partially due to increased apoptosis. Residual NK cells showed lower expression of activating Ly49 receptors and normal (liver) or decreased (spleen) IFN-gamma production. Nevertheless, NK cells from CD70-Tg mice displayed higher YAC-1 killing capacities. CD70-Tg NK cells exhibited up-regulated expression of NKG2D, which is in accordance with the increased YAC-1 lysis, as this is mainly NKG2D-dependent. Taken together, this study is the first to demonstrate that continuous CD70 triggering of CD27 on NK cells in vivo results in a severe reduction of NK cells. On a single cell basis, however, residual NK cells display enhanced cytotoxicity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RECEPTOR, EXPRESSION, TUMOR-CELLS, ACTIVATION, TARGET-CELLS, GAMMA PRODUCTION, T-CELL, NATURAL-KILLER-CELL, PROGENITORS, MURINE BONE-MARROW, Differentiation, Cytotoxicity, CD70-Tg mice
journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
Eur. J. Immunol.
volume
40
issue
4
pages
1107 - 1117
Web of Science type
Article
Web of Science id
000277210800021
JCR category
IMMUNOLOGY
JCR impact factor
4.942 (2010)
JCR rank
22/133 (2010)
JCR quartile
1 (2010)
ISSN
0014-2980
DOI
10.1002/eji.200939251
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
959122
handle
http://hdl.handle.net/1854/LU-959122
date created
2010-05-31 09:18:03
date last changed
2016-12-19 15:40:14
@article{959122,
  abstract     = {NK cells are important mediators of the early defense. In mice, immature and mature NK (mNK) cells constitutively express the TNF receptor family member CD27; however, mNK cells eventually lose CD27 expression and become resting NK cells. Interaction of CD27 with its ligand, CD70, enhances proliferation and effector functions of NK cells. We used mice that constitutively express CD70 on B cells (CD70-Tg) to study the in vivo effects of continuous triggering of CD27 on NK cells. Continuous CD70-CD27 interaction resulted in strongly down-modulated CD27 expression on NK cells and gradually reduced absolute NK cell numbers. This reduction was most prominent in the mNK cell subpopulation and was at least partially due to increased apoptosis. Residual NK cells showed lower expression of activating Ly49 receptors and normal (liver) or decreased (spleen) IFN-gamma production. Nevertheless, NK cells from CD70-Tg mice displayed higher YAC-1 killing capacities. CD70-Tg NK cells exhibited up-regulated expression of NKG2D, which is in accordance with the increased YAC-1 lysis, as this is mainly NKG2D-dependent. Taken together, this study is the first to demonstrate that continuous CD70 triggering of CD27 on NK cells in vivo results in a severe reduction of NK cells. On a single cell basis, however, residual NK cells display enhanced cytotoxicity.},
  author       = {De Colvenaer, Veerle and Taveirne, Sylvie and Hamann, J{\"o}rg and de Bruin, Alex M and De Smedt, Magda and Taghon, Tom and Vandekerckhove, Bart and Plum, Jean and van Lier, Ren{\'e} and Leclercq, Georges},
  issn         = {0014-2980},
  journal      = {EUROPEAN JOURNAL OF IMMUNOLOGY},
  keyword      = {RECEPTOR,EXPRESSION,TUMOR-CELLS,ACTIVATION,TARGET-CELLS,GAMMA PRODUCTION,T-CELL,NATURAL-KILLER-CELL,PROGENITORS,MURINE BONE-MARROW,Differentiation,Cytotoxicity,CD70-Tg mice},
  language     = {eng},
  number       = {4},
  pages        = {1107--1117},
  title        = {Continuous CD27 triggering in vivo strongly reduces NK cell numbers},
  url          = {http://dx.doi.org/10.1002/eji.200939251},
  volume       = {40},
  year         = {2010},
}

Chicago
De Colvenaer, Veerle, Sylvie Taveirne, Jörg Hamann, Alex M de Bruin, Magda De Smedt, Tom Taghon, Bart Vandekerckhove, Jean Plum, René van Lier, and Georges Leclercq. 2010. “Continuous CD27 Triggering in Vivo Strongly Reduces NK Cell Numbers.” European Journal of Immunology 40 (4): 1107–1117.
APA
De Colvenaer, V., Taveirne, S., Hamann, J., de Bruin, A. M., De Smedt, M., Taghon, T., Vandekerckhove, B., et al. (2010). Continuous CD27 triggering in vivo strongly reduces NK cell numbers. EUROPEAN JOURNAL OF IMMUNOLOGY, 40(4), 1107–1117.
Vancouver
1.
De Colvenaer V, Taveirne S, Hamann J, de Bruin AM, De Smedt M, Taghon T, et al. Continuous CD27 triggering in vivo strongly reduces NK cell numbers. EUROPEAN JOURNAL OF IMMUNOLOGY. 2010;40(4):1107–17.
MLA
De Colvenaer, Veerle, Sylvie Taveirne, Jörg Hamann, et al. “Continuous CD27 Triggering in Vivo Strongly Reduces NK Cell Numbers.” EUROPEAN JOURNAL OF IMMUNOLOGY 40.4 (2010): 1107–1117. Print.