Advanced search
1 file | 487.37 KB Add to list

Gene function analysis by artificial MicroRNAs in Physcomitrella patens

Author
Organization
Abstract
MicroRNAs (miRNAs) are ~21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.
Keywords
gene silencing, Pyscomitrella patens, gene knockdown, microRNA, artificial microRNA, RNAi

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 487.37 KB

Citation

Please use this url to cite or link to this publication:

MLA
Khraiwesh, Basel, Isam Fattash, M Asif Arif, et al. “Gene Function Analysis by Artificial MicroRNAs in Physcomitrella Patens.” RNAi and Plant Gene Function Analysis : Methods and Protocols. Ed. Hiroaki Kodama & Atsushi Komamine. Vol. 744. New York, NY, USA: Springer, 2011. 57–79. Print.
APA
Khraiwesh, B., Fattash, I., Arif, M. A., & Frank, W. (2011). Gene function analysis by artificial MicroRNAs in Physcomitrella patens. In H. Kodama & A. Komamine (Eds.), RNAi and plant gene function analysis : methods and protocols (Vol. 744, pp. 57–79). New York, NY, USA: Springer.
Chicago author-date
Khraiwesh, Basel, Isam Fattash, M Asif Arif, and Wolfgang Frank. 2011. “Gene Function Analysis by Artificial MicroRNAs in Physcomitrella Patens.” In RNAi and Plant Gene Function Analysis : Methods and Protocols, ed. Hiroaki Kodama and Atsushi Komamine, 744:57–79. New York, NY, USA: Springer.
Chicago author-date (all authors)
Khraiwesh, Basel, Isam Fattash, M Asif Arif, and Wolfgang Frank. 2011. “Gene Function Analysis by Artificial MicroRNAs in Physcomitrella Patens.” In RNAi and Plant Gene Function Analysis : Methods and Protocols, ed. Hiroaki Kodama and Atsushi Komamine, 744:57–79. New York, NY, USA: Springer.
Vancouver
1.
Khraiwesh B, Fattash I, Arif MA, Frank W. Gene function analysis by artificial MicroRNAs in Physcomitrella patens. In: Kodama H, Komamine A, editors. RNAi and plant gene function analysis : methods and protocols. New York, NY, USA: Springer; 2011. p. 57–79.
IEEE
[1]
B. Khraiwesh, I. Fattash, M. A. Arif, and W. Frank, “Gene function analysis by artificial MicroRNAs in Physcomitrella patens,” in RNAi and plant gene function analysis : methods and protocols, vol. 744, H. Kodama and A. Komamine, Eds. New York, NY, USA: Springer, 2011, pp. 57–79.
@incollection{956235,
  abstract     = {MicroRNAs (miRNAs) are ~21nt long small RNAs transcribed from endogenous MIR genes which form precursor RNAs with a characteristic hairpin structure. MiRNAs control the expression of cognate target genes by binding to reverse complementary sequences resulting in cleavage or translational inhibition of the target RNA. Artificial miRNAs (amiRNAs) can be generated by exchanging the miRNA/miRNA* sequence of endogenous MIR precursor genes, while maintaining the general pattern of matches and mismatches in the foldback. Thus, for functional gene analysis amiRNAs can be designed to target any gene of interest.},
  author       = {Khraiwesh, Basel and Fattash, Isam  and Arif, M Asif  and Frank, Wolfgang },
  booktitle    = {RNAi and plant gene function analysis : methods and protocols},
  editor       = {Kodama, Hiroaki  and Komamine, Atsushi},
  isbn         = {9781617791239},
  issn         = {1064-3745},
  keywords     = {gene silencing,Pyscomitrella patens,gene knockdown,microRNA,artificial microRNA,RNAi},
  language     = {eng},
  pages        = {57--79},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Gene function analysis by artificial MicroRNAs in Physcomitrella patens},
  url          = {http://dx.doi.org/10.1007/978-1-61779-123-9_5},
  volume       = {744},
  year         = {2011},
}

Altmetric
View in Altmetric