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Transcriptional control of gene expression by MicroRNAs

(2010) CELL. 140(1). p.111-122
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Abstract
MicroRNAs (miRNAs) control gene expression in animals and plants. Like another class of small RNAs, siRNAs, they affect gene expression posttranscriptionally. While siRNAs in addition act in transcriptional gene silencing, a role of miRNAs in transcriptional regulation has been less clear. We show here that in moss Physcomitrella patens mutants without a DICER-LIKE1b gene, maturation of miRNAs is normal but cleavage of target RNAs is abolished and levels of these transcripts are drastically reduced. These mutants accumulate miRNA: target-RNA duplexes and show hypermethylation of the genes encoding target RNAs, leading to gene silencing. This pathway occurs also in the wild-type upon hormone treatment. We propose that initiation of epigenetic silencing by DNA methylation depends on the ratio of the miRNA and its target RNA.
Keywords
PLANTS, DICER HOMOLOG, MESSENGER-RNAS, MAMMALIAN-CELLS, C-ELEGANS, ARABIDOPSIS-THALIANA, PHYSCOMITRELLA-PATENS, TRANS-ACTING SIRNAS, DEPENDENT RNA-POLYMERASE, DIRECTED DNA METHYLATION

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Citation

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Chicago
Khraiwesh, Basel, M Asif Arif, Gotelinde I Seumel, Stephan Ossowski, Detlef Weigel, Ralf Reski, and Wolfgang Frank. 2010. “Transcriptional Control of Gene Expression by MicroRNAs.” Cell 140 (1): 111–122.
APA
Khraiwesh, B., Arif, M. A., Seumel, G. I., Ossowski, S., Weigel, D., Reski, R., & Frank, W. (2010). Transcriptional control of gene expression by MicroRNAs. CELL, 140(1), 111–122.
Vancouver
1.
Khraiwesh B, Arif MA, Seumel GI, Ossowski S, Weigel D, Reski R, et al. Transcriptional control of gene expression by MicroRNAs. CELL. 2010;140(1):111–22.
MLA
Khraiwesh, Basel, M Asif Arif, Gotelinde I Seumel, et al. “Transcriptional Control of Gene Expression by MicroRNAs.” CELL 140.1 (2010): 111–122. Print.
@article{956199,
  abstract     = {MicroRNAs (miRNAs) control gene expression in animals and plants. Like another class of small RNAs, siRNAs, they affect gene expression posttranscriptionally. While siRNAs in addition act in transcriptional gene silencing, a role of miRNAs in transcriptional regulation has been less clear. We show here that in moss Physcomitrella patens mutants without a DICER-LIKE1b gene, maturation of miRNAs is normal but cleavage of target RNAs is abolished and levels of these transcripts are drastically reduced. These mutants accumulate miRNA: target-RNA duplexes and show hypermethylation of the genes encoding target RNAs, leading to gene silencing. This pathway occurs also in the wild-type upon hormone treatment. We propose that initiation of epigenetic silencing by DNA methylation depends on the ratio of the miRNA and its target RNA.},
  author       = {Khraiwesh, Basel and Arif, M Asif  and Seumel, Gotelinde I and Ossowski, Stephan  and Weigel, Detlef  and Reski, Ralf  and  Frank, Wolfgang},
  issn         = {0092-8674},
  journal      = {CELL},
  keyword      = {PLANTS,DICER HOMOLOG,MESSENGER-RNAS,MAMMALIAN-CELLS,C-ELEGANS,ARABIDOPSIS-THALIANA,PHYSCOMITRELLA-PATENS,TRANS-ACTING SIRNAS,DEPENDENT RNA-POLYMERASE,DIRECTED DNA METHYLATION},
  language     = {eng},
  number       = {1},
  pages        = {111--122},
  title        = {Transcriptional control of gene expression by MicroRNAs},
  url          = {http://dx.doi.org/10.1016/j.cell.2009.12.023},
  volume       = {140},
  year         = {2010},
}

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