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Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei

Luciane Chimetto Tonon UGent, Ilse Cleenwerck UGent, Nelson Alves Junior, Bruno Sergio Silva, Marcelo Brocchi, Anne Willems UGent, Paul De Vos UGent and Fabiano L Thompson (2011) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 61(2). p.362-368
abstract
Eight Vibrio isolates originating from the marine corals Mussismilia hispida and Phyllogorgia dilatata and the zoanthids Palythoa caribaeorum and Palythoa variabilis in Brazil and the Pacific white shrimp (Litopenaeus vannamei) in Ecuador were studied by means of a polyphasic approach. The novel isolates formed a tight monophyletic group in the genus Vibrio and were closely related to species of the Vibrio harveyi group, to which they showed more than 99% 16S rRNA gene sequence similarity. Analysis based on concatenated sequences of the following seven genes, 16S rRNA, gyrB, recA, rpoA, topA, pyrH and mreB (5633 bp in length), showed clear separation between the isolates and species of the V. harveyi group. Amplified fragment length polymorphism (AFLP) analysis, performed previously, revealed that a representative isolate of this group, LMG 20370, was clearly separate from known Vibrio species (it belonged to the so-called AFLP cluster A31). DNA-DNA hybridization (DDH) experiments with representative isolates and type strains of the V. harveyi species group revealed high DDH between the novel isolates (more than 74 %) and less than 70% DDH towards type strains of related Vibrio species, proving the novel species status of the isolates. Phenotypically, the novel species belongs to the arginine dihydrolase (A)-negative, lysine decarboxylase (L)-positive and ornithine decarboxylase (O)-positive (A-/L+/O+) cluster reported previously. Most species of the V. harveyi group (i.e. Vibrio rotiferianus, V. harveyi, V. parahaemolyticus and V. alginolyticus) also belong to this A-/L+/O+ cluster. However, several phenotypic features can be used for the identification of the novel species. In contrast to its closest phylogenetic neighbours, the novel species exhibits esterase (C4) and N-acetyl-beta-glucosaminidase activities, but it does not produce acetoin, does not use citrate, a-ketoglutaric acid or propionic acid and does not ferment melibiose. The novel species can also be differentiated on the basis of the presence of the fatty acids C(17:0), C(17:1)omega 8c, iso-C(17:0) and iso-C(13:0) and the absence of the fatty acid C(18:0). The name Vibrio communis sp. nov. is proposed for this taxon. Strain R-40496(T) (=LMG 25430(T) =CAIM 1816(T)) is the type strain.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GEN. NOV., MULTILOCUS SEQUENCE-ANALYSIS, MOLECULAR-IDENTIFICATION, DEOXYRIBONUCLEIC-ACID, GENOMIC DIVERSITY, COMB. NOV, HYBRIDIZATION, BACTERIUM, HARVEYI, RECLASSIFICATION
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
61
issue
2
pages
362 - 368
Web of Science type
Article
Web of Science id
000288471300026
JCR category
MICROBIOLOGY
JCR impact factor
2.268 (2011)
JCR rank
63/111 (2011)
JCR quartile
3 (2011)
ISSN
1466-5026
DOI
10.1099/ijs.0.019729-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
953068
handle
http://hdl.handle.net/1854/LU-953068
date created
2010-05-26 13:56:06
date last changed
2011-07-26 16:28:52
@article{953068,
  abstract     = {Eight Vibrio isolates originating from the marine corals Mussismilia hispida and Phyllogorgia dilatata and the zoanthids Palythoa caribaeorum and Palythoa variabilis in Brazil and the Pacific white shrimp (Litopenaeus vannamei) in Ecuador were studied by means of a polyphasic approach. The novel isolates formed a tight monophyletic group in the genus Vibrio and were closely related to species of the Vibrio harveyi group, to which they showed more than 99\% 16S rRNA gene sequence similarity. Analysis based on concatenated sequences of the following seven genes, 16S rRNA, gyrB, recA, rpoA, topA, pyrH and mreB (5633 bp in length), showed clear separation between the isolates and species of the V. harveyi group. Amplified fragment length polymorphism (AFLP) analysis, performed previously, revealed that a representative isolate of this group, LMG 20370, was clearly separate from known Vibrio species (it belonged to the so-called AFLP cluster A31). DNA-DNA hybridization (DDH) experiments with representative isolates and type strains of the V. harveyi species group revealed high DDH between the novel isolates (more than 74 \%) and less than 70\% DDH towards type strains of related Vibrio species, proving the novel species status of the isolates. Phenotypically, the novel species belongs to the arginine dihydrolase (A)-negative, lysine decarboxylase (L)-positive and ornithine decarboxylase (O)-positive (A-/L+/O+) cluster reported previously. Most species of the V. harveyi group (i.e. Vibrio rotiferianus, V. harveyi, V. parahaemolyticus and V. alginolyticus) also belong to this A-/L+/O+ cluster. However, several phenotypic features can be used for the identification of the novel species. In contrast to its closest phylogenetic neighbours, the novel species exhibits esterase (C4) and N-acetyl-beta-glucosaminidase activities, but it does not produce acetoin, does not use citrate, a-ketoglutaric acid or propionic acid and does not ferment melibiose. The novel species can also be differentiated on the basis of the presence of the fatty acids C(17:0), C(17:1)omega 8c, iso-C(17:0) and iso-C(13:0) and the absence of the fatty acid C(18:0). The name Vibrio communis sp. nov. is proposed for this taxon. Strain R-40496(T) (=LMG 25430(T) =CAIM 1816(T)) is the type strain.},
  author       = {Chimetto Tonon, Luciane and Cleenwerck, Ilse and Junior, Nelson Alves and Silva, Bruno Sergio and Brocchi, Marcelo and Willems, Anne and De Vos, Paul and Thompson, Fabiano L},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {GEN. NOV.,MULTILOCUS SEQUENCE-ANALYSIS,MOLECULAR-IDENTIFICATION,DEOXYRIBONUCLEIC-ACID,GENOMIC DIVERSITY,COMB. NOV,HYBRIDIZATION,BACTERIUM,HARVEYI,RECLASSIFICATION},
  language     = {eng},
  number       = {2},
  pages        = {362--368},
  title        = {Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei},
  url          = {http://dx.doi.org/10.1099/ijs.0.019729-0},
  volume       = {61},
  year         = {2011},
}

Chicago
Chimetto Tonon, Luciane, Ilse Cleenwerck, Nelson Alves Junior, Bruno Sergio Silva, Marcelo Brocchi, Anne Willems, Paul De Vos, and Fabiano L Thompson. 2011. “Vibrio Communis Sp. Nov., Isolated from the Marine Animals Mussismilia Hispida, Phyllogorgia Dilatata, Palythoa Caribaeorum, Palythoa Variabilis and Litopenaeus Vannamei.” International Journal of Systematic and Evolutionary Microbiology 61 (2): 362–368.
APA
Chimetto Tonon, L., Cleenwerck, I., Junior, N. A., Silva, B. S., Brocchi, M., Willems, A., De Vos, P., et al. (2011). Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 61(2), 362–368.
Vancouver
1.
Chimetto Tonon L, Cleenwerck I, Junior NA, Silva BS, Brocchi M, Willems A, et al. Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2011;61(2):362–8.
MLA
Chimetto Tonon, Luciane, Ilse Cleenwerck, Nelson Alves Junior, et al. “Vibrio Communis Sp. Nov., Isolated from the Marine Animals Mussismilia Hispida, Phyllogorgia Dilatata, Palythoa Caribaeorum, Palythoa Variabilis and Litopenaeus Vannamei.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 61.2 (2011): 362–368. Print.