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Prophylactic use of transferrins against chlamydiosis

Delphine Sylvie Anne Beeckman UGent, Caroline Van Droogenbroeck UGent and Daisy Vanrompay UGent (2010) Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International conference, Abstracts.
abstract
Introduction: Chlamydophila psittaci is an important poultry pathogen, especially in turkeys and ducks, causing infections of mucosal epithelial cells and macrophages of the respiratory tract, followed by septicaemia and localisation in epithelial cells and macrophages in various organs. Chlamydial infections in poultry not only present significant economical losses, public health is also under consideration since veterinary surgeons and poultry workers are at high risk of becoming infected by this zoonotic agent. Objectives: the effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular Gram-negative bacterium Chlamydophila (Cp.) psittaci was evaluated using a model of African Green Monkey kidney (BGM) cells and chicken macrophages (HD11 cells) as artificial hosts. Subsequently, the potential use of ovotransferrin (ovoTF) to prevent chlamydiosis in vivo was evaluated using experimentally infected SPF turkeys. Results: firstly, the effect of transferrins on extracellular bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/ml ovoTF prior to infecting BGM cells significantly lowered the infection rate. For both lactoferrins, the infection rate could only be reduced with 5 mg/ml, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/ml ovoTF and 0.5 mg/ml hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. Thirdly, the effect of ovoTF was evaluated in vivo. A single dose of 10 mg aerosolized ovoTF per turkey prior to infection and a daily dose of 5 mg ovoTF during twelve subsequent days provided significant protection regarding clinical signs, gross lesions and chlamydial replication and excretion. Interestingly, there was a clear effect of the administered dose of ovoTF as turkeys receiving 5 mg ovoTF per turkey only once, showed severe symptoms comparable to those of the untreated control group. The present results demonstrate the anti-chlamydial effect of ovoTF in vivo and indicate the possibility to administer this anti-microbial protein aerogenically to poultry in order to prevent Cp. psittaci infections. Conclusion: The present results demonstrate the anti-chlamydial effect of ovoTF in vivo and indicate the possibility to administer this anti-microbial protein aerogenically to poultry in order to prevent Cp. psittaci infections.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International conference, Abstracts
conference name
2nd International conference on Modern Mucosal Vaccines, Adjuvants and Microbicides (MMVAM 2010)
conference location
Dublin, Ireland
conference start
2010-04-28
conference end
2010-04-30
language
English
UGent publication?
yes
classification
C3
additional info
publication on CD-Rom
id
948680
handle
http://hdl.handle.net/1854/LU-948680
date created
2010-05-19 10:36:22
date last changed
2016-12-19 15:36:01
@inproceedings{948680,
  abstract     = {Introduction: Chlamydophila psittaci is an important poultry pathogen, especially in turkeys and ducks, causing infections of mucosal epithelial cells and macrophages of the respiratory tract, followed by septicaemia and localisation in epithelial cells and macrophages in various organs. Chlamydial infections in poultry not only present significant economical losses, public health is also under consideration since veterinary surgeons and poultry workers are at high risk of becoming infected by this zoonotic agent.
Objectives: the effect of ovotransferrin (ovoTF), human lactoferrin (hLF) and bovine lactoferrin (bLF) on the obligate intracellular Gram-negative bacterium Chlamydophila (Cp.) psittaci was evaluated using a model of African Green Monkey kidney (BGM) cells and chicken macrophages (HD11 cells) as artificial hosts. Subsequently, the potential use of ovotransferrin (ovoTF) to prevent chlamydiosis in vivo was evaluated using experimentally infected SPF turkeys.
Results: firstly, the effect of transferrins on extracellular bacteria was evaluated. Pre-incubation of Cp. psittaci with 0.5 to 5 mg/ml ovoTF prior to infecting BGM cells significantly lowered the infection rate. For both lactoferrins, the infection rate could only be reduced with 5 mg/ml, albeit not significantly as compared to the infection rate created by the untreated bacteria. Secondly, transferrins were tested for their ability to influence bacterial adhesion and entry in HD11 cells. Maximal non-cytotoxic and non-bactericidal concentrations of 0.05 mg/ml ovoTF and 0.5 mg/ml hLF and bLF were used. Overall, ovoTF was more effective than human and bovine LF in inhibiting bacterial attachment and cell entry and the latter was accompanied by a dose-dependent reduction of actin recruitment at the bacterial entry site. However, once bacteria had entered HD11 cells, transferrins had apparently no effect on intracellular replication. Thirdly, the effect of ovoTF was evaluated in vivo. A single dose of 10 mg aerosolized ovoTF per turkey prior to infection and a daily dose of 5 mg ovoTF during twelve subsequent days provided significant protection regarding clinical signs, gross lesions and chlamydial replication and excretion. Interestingly, there was a clear effect of the administered dose of ovoTF as turkeys receiving 5 mg ovoTF per turkey only once, showed severe symptoms comparable to those of the untreated control group. The present results demonstrate the anti-chlamydial effect of ovoTF in vivo and indicate the possibility to administer this anti-microbial protein aerogenically to poultry in order to prevent Cp. psittaci infections.
Conclusion: The present results demonstrate the anti-chlamydial effect of ovoTF in vivo and indicate the possibility to administer this anti-microbial protein aerogenically to poultry in order to prevent Cp. psittaci infections.},
  author       = {Beeckman, Delphine Sylvie Anne and Van Droogenbroeck, Caroline and Vanrompay, Daisy},
  booktitle    = {Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International conference, Abstracts},
  language     = {eng},
  location     = {Dublin, Ireland},
  title        = {Prophylactic use of transferrins against chlamydiosis},
  year         = {2010},
}

Chicago
Beeckman, Delphine Sylvie Anne, Caroline Van Droogenbroeck, and Daisy Vanrompay. 2010. “Prophylactic Use of Transferrins Against Chlamydiosis.” In Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International Conference, Abstracts.
APA
Beeckman, D. S. A., Van Droogenbroeck, C., & Vanrompay, D. (2010). Prophylactic use of transferrins against chlamydiosis. Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International conference, Abstracts. Presented at the 2nd International conference on Modern Mucosal Vaccines, Adjuvants and Microbicides (MMVAM 2010).
Vancouver
1.
Beeckman DSA, Van Droogenbroeck C, Vanrompay D. Prophylactic use of transferrins against chlamydiosis. Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International conference, Abstracts. 2010.
MLA
Beeckman, Delphine Sylvie Anne, Caroline Van Droogenbroeck, and Daisy Vanrompay. “Prophylactic Use of Transferrins Against Chlamydiosis.” Modern Mucosal Vaccines, Adjuvants and Microbicides, 2nd International Conference, Abstracts. 2010. Print.