Ghent University Academic Bibliography

Advanced

Development of Gantrez® AN nanoparticles for oral delivery of antigens

Katrien Vandamme UGent (2010)
abstract
The development of a dosage form that improves the absorption of vaccines via the gastrointestinal tract is one of the greatest challenges in the pharmaceutical field. On the one hand, oral vaccine administration is favoured, thanks to its non-invasive character. In addition, oral administration of vaccines allows the induction of a mucosal immune response, which is advantageous since most pathogens enter the body via mucosal surfaces. On the other hand, the oral route includes several challenges for vaccine delivery as the harsh conditions in the gastrointestinal tract can cause antigen degradation and oral absorption of intact antigens is usually poor. To protect antigens against chemical, enzymatic or immunological degradation, polymeric nanoparticulate delivery systems have been proposed. Moreover, to enhance antigen uptake, nanoparticle coating with targeting ligands is of special interest, as ligands can interact with specific receptors on epithelial cells, resulting in receptor-mediated uptake and transport through the epithelial barrier. In this field, the biocompatible copolymer between methylvinylether and maleic anhydride (commercialised as Gantrez®AN) has been proposed as carrier. Using a solvent displacement or nanoprecipitation method, ligand-bound nanoparticles are easily formulated, due to the presence of reactive anhydride groups which do not need time-consuming chemical activation. Formulation of antigen-containing Gantrez®AN nanoparticles via the solvent displacement method requires the use of an organic solvent (e.g. acetone). However, as antigens are hydrophilic entities this technique is not universally applicable. In order to broaden the application of Gantrez®AN-based nanoparticles, an alternative synthesis method was developed in Chapter II.2 in which water-soluble antigens were dissolved in a water phase prior to mixing with an organic Gantrez®AN solution (using β-galactosidase as model antigen).
Please use this url to cite or link to this publication:
author
promoter
UGent and UGent
organization
year
type
dissertation (monograph)
subject
keyword
poly methylvinylether-co-maleic anhydride, Gantrez ® AN, vaccination, mucosal, nanoparticles
pages
201 pages
publisher
Ghent University. Faculty of Pharmaceutical Sciences
place of publication
Ghent, Belgium
defense location
Gent : Farmaceutisch Instituut (auditorium II)
defense date
2010-05-06 17:00
language
English
UGent publication?
yes
classification
D1
additional info
dissertation in parts contains copyrighted material
copyright statement
I have transferred the copyright for this publication to the publisher
id
944304
handle
http://hdl.handle.net/1854/LU-944304
alternative location
http://lib.ugent.be/fulltxt/RUG01/001/401/469/RUG01-001401469_2010_0001_AC.pdf
date created
2010-05-10 09:44:48
date last changed
2010-05-18 16:50:09
@phdthesis{944304,
  abstract     = {The development of a dosage form that improves the absorption of vaccines via the gastrointestinal tract is one of the greatest challenges in the pharmaceutical field. On the one hand, oral vaccine administration is favoured, thanks to its non-invasive character. In addition, oral administration of vaccines allows the induction of a mucosal immune response, which is advantageous since most pathogens enter the body via mucosal surfaces. On the other hand, the oral route includes several challenges for vaccine delivery as the harsh conditions in the gastrointestinal tract can cause antigen degradation and oral absorption of intact antigens is usually poor.
To protect antigens against chemical, enzymatic or immunological degradation, polymeric nanoparticulate delivery systems have been proposed. Moreover, to enhance antigen uptake, nanoparticle coating with targeting ligands is of special interest, as ligands can interact with specific receptors on epithelial cells, resulting in receptor-mediated uptake and transport through the epithelial barrier.
In this field, the biocompatible copolymer between methylvinylether and maleic anhydride (commercialised as Gantrez{\textregistered}AN) has been proposed as carrier. Using a solvent displacement or nanoprecipitation method, ligand-bound nanoparticles are easily formulated, due to the presence of reactive anhydride groups which do not need time-consuming chemical activation. Formulation of antigen-containing Gantrez{\textregistered}AN nanoparticles via the solvent displacement method requires the use of an organic solvent (e.g. acetone). However, as antigens are hydrophilic entities this technique is not universally applicable.
In order to broaden the application of Gantrez{\textregistered}AN-based nanoparticles, an alternative synthesis method was developed in Chapter II.2 in which water-soluble antigens were dissolved in a water phase prior to mixing with an organic Gantrez{\textregistered}AN solution (using \ensuremath{\beta}-galactosidase as model antigen).},
  author       = {Vandamme, Katrien},
  keyword      = {poly methylvinylether-co-maleic anhydride,Gantrez {\textregistered} AN,vaccination,mucosal,nanoparticles},
  language     = {eng},
  pages        = {201},
  publisher    = {Ghent University. Faculty of Pharmaceutical Sciences},
  school       = {Ghent University},
  title        = {Development of Gantrez{\textregistered} AN nanoparticles for oral delivery of antigens},
  url          = {http://lib.ugent.be/fulltxt/RUG01/001/401/469/RUG01-001401469\_2010\_0001\_AC.pdf},
  year         = {2010},
}

Chicago
Vandamme, Katrien. 2010. “Development of Gantrez® AN Nanoparticles for Oral Delivery of Antigens”. Ghent, Belgium: Ghent University. Faculty of Pharmaceutical Sciences.
APA
Vandamme, Katrien. (2010). Development of Gantrez® AN nanoparticles for oral delivery of antigens. Ghent University. Faculty of Pharmaceutical Sciences, Ghent, Belgium.
Vancouver
1.
Vandamme K. Development of Gantrez® AN nanoparticles for oral delivery of antigens. [Ghent, Belgium]: Ghent University. Faculty of Pharmaceutical Sciences; 2010.
MLA
Vandamme, Katrien. “Development of Gantrez® AN Nanoparticles for Oral Delivery of Antigens.” 2010 : n. pag. Print.