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Identification of lysine positive non-fermenting gram negative bacilli (Stenotrophomonas maltophilia and Burkholderia cepacia complex)

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Abstract
Background: The Burkholderia cepacia complex (BCC) and Stenotrophomonas maltophilia are closely related groups of non-fermenting gram-negative bacilli (NFGNBs) having a similar spectrum of infections ranging from superficial to deep-seated and disseminated infections. Identification of these lysine decarboxylase-positive NFGNBs lags behind in most Indian laboratories. A simplified identification scheme was devised for these two pathogens that allowed us to isolate them with an increasing frequency at our tertiary care institute. Materials and Methods: A simple five-tube conventional biochemical identification of these bacteria has been standardized. In the beginning, some of the isolates were confirmed from the International B. cepacia Working group, Belgium. Molecular identification and typing using recA polymerase chain reaction-restriction fragment length polymorphism was also standardized for BCC. For short-term preservation of BCC, an innovative method of preserving the bacteria in Robertsons cooked medium tubes kept in a domestic refrigerator was developed. Results: Thirty-nine isolates of BCC isolates were obtained from various specimens (30 from blood cultures) and 22 S. maltophilia (13 blood cultures and 9 respiratory isolates) were isolated during the year 2007 alone. Conclusions: BCC and S. maltophilia can be identified with relative ease using a small battery of biochemical reactions. Use of simplified methods will allow greater recognition of their pathogenic potential and correct antimicrobials should be advised in other clinical laboratories and hospitals.
Keywords
India, stenotrophomonas maltophilia, identification, Burkholderia cepacia complex, CENOCEPACIA, BACTEREMIA, CYSTIC-FIBROSIS PATIENTS, GENOMOVAR STATUS, INFECTION, OUTBREAK

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Chicago
Gautam, V, P Ray, Peter Vandamme, SS Chatterjee, A Das, K Sharma, S Rana, et al. 2009. “Identification of Lysine Positive Non-fermenting Gram Negative Bacilli (Stenotrophomonas Maltophilia and Burkholderia Cepacia Complex).” Indian Journal of Medical Microbiology 27 (2): 128–133.
APA
Gautam, V, Ray, P., Vandamme, P., Chatterjee, S., Das, A., Sharma, K., Rana, S., et al. (2009). Identification of lysine positive non-fermenting gram negative bacilli (Stenotrophomonas maltophilia and Burkholderia cepacia complex). INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 27(2), 128–133.
Vancouver
1.
Gautam V, Ray P, Vandamme P, Chatterjee S, Das A, Sharma K, et al. Identification of lysine positive non-fermenting gram negative bacilli (Stenotrophomonas maltophilia and Burkholderia cepacia complex). INDIAN JOURNAL OF MEDICAL MICROBIOLOGY. 2009;27(2):128–33.
MLA
Gautam, V, P Ray, Peter Vandamme, et al. “Identification of Lysine Positive Non-fermenting Gram Negative Bacilli (Stenotrophomonas Maltophilia and Burkholderia Cepacia Complex).” INDIAN JOURNAL OF MEDICAL MICROBIOLOGY 27.2 (2009): 128–133. Print.
@article{943201,
  abstract     = {Background: The Burkholderia cepacia complex (BCC) and Stenotrophomonas maltophilia are closely related groups of non-fermenting gram-negative bacilli (NFGNBs) having a similar spectrum of infections ranging from superficial to deep-seated and disseminated infections. Identification of these lysine decarboxylase-positive NFGNBs lags behind in most Indian laboratories. A simplified identification scheme was devised for these two pathogens that allowed us to isolate them with an increasing frequency at our tertiary care institute. Materials and Methods: A simple five-tube conventional biochemical identification of these bacteria has been standardized. In the beginning, some of the isolates were confirmed from the International B. cepacia Working group, Belgium. Molecular identification and typing using recA polymerase chain reaction-restriction fragment length polymorphism was also standardized for BCC. For short-term preservation of BCC, an innovative method of preserving the bacteria in Robertsons cooked medium tubes kept in a domestic refrigerator was developed. Results: Thirty-nine isolates of BCC isolates were obtained from various specimens (30 from blood cultures) and 22 S. maltophilia (13 blood cultures and 9 respiratory isolates) were isolated during the year 2007 alone. Conclusions: BCC and S. maltophilia can be identified with relative ease using a small battery of biochemical reactions. Use of simplified methods will allow greater recognition of their pathogenic potential and correct antimicrobials should be advised in other clinical laboratories and hospitals.},
  author       = {Gautam, V and Ray, P and Vandamme, Peter and Chatterjee, SS and Das, A and Sharma, K and Rana, S and Garg, RK and Madhup, SK and Mahajan, M and Sharma, M},
  issn         = {0255-0857},
  journal      = {INDIAN JOURNAL OF MEDICAL MICROBIOLOGY},
  language     = {eng},
  number       = {2},
  pages        = {128--133},
  title        = {Identification of lysine positive non-fermenting gram negative bacilli (Stenotrophomonas maltophilia and Burkholderia cepacia complex)},
  url          = {http://dx.doi.org/10.4103/0255-0857.49425},
  volume       = {27},
  year         = {2009},
}

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