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Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite

Elke Peeters UGent, Andrea Sass, Eshwar Mahenthiralingam, Hans Nelis UGent and Tom Coenye UGent (2010) BMC GENOMICS. 11.
abstract
Background: Burkholderia cepacia complex bacteria are opportunistic pathogens, which can cause severe respiratory tract infections in patients with cystic fibrosis (CF). As treatment of infected CF patients is problematic, multiple preventive measures are taken to reduce the infection risk. Besides a stringent segregation policy to prevent patient-to-patient transmission, clinicians also advise patients to clean and disinfect their respiratory equipment on a regular basis. However, problems regarding the efficacy of several disinfection procedures for the removal and/or killing of B. cepacia complex bacteria have been reported. In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of reactive oxygen species (ROS), the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2 or NaOCl. Results: The exposure to H2O2 and NaOCl resulted in an upregulation of the transcription of 315 (4.4%) and 386 (5.4%) genes, respectively. Transcription of 185 (2.6%) and 331 (4.6%) genes was decreased in response to the respective treatments. Many of the upregulated genes in the NaOCl- and H2O2-treated biofilms are involved in oxidative stress as well as general stress response, emphasizing the importance of the efficient neutralization and scavenging of ROS. In addition, multiple upregulated genes encode proteins that are necessary to repair ROS-induced cellular damage. Unexpectedly, a prolonged treatment with H2O2 also resulted in an increased transcription of multiple phage-related genes. A closer inspection of hybridisation signals obtained with probes targeting intergenic regions led to the identification of a putative 6S RNA. Conclusion: Our results reveal that the transcription of a large fraction of B. cenocepacia J2315 genes is altered upon exposure of sessile cells to ROS. These observations have highlighted that B. cenocepacia may alter several pathways in response to exposure to ROS and they have led to the identification of many genes not previously implicated in the stress response of this pathogen.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYSTIC-FIBROSIS PATIENTS, ALKYL HYDROPEROXIDE REDUCTASE, QUALITY-CONTROL MAQC, PSEUDOMONAS-AERUGINOSA, ESCHERICHIA-COLI, CEPACIA COMPLEX, OXIDATIVE STRESS, ORGANIC HYDROPEROXIDE, SIGMA-FACTOR, BIOFILM FORMATION
journal title
BMC GENOMICS
BMC Genomics
volume
11
Web of Science type
Article
Web of Science id
000275291800001
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
4.206 (2010)
JCR rank
24/158 (2010)
JCR quartile
1 (2010)
ISSN
1471-2164
DOI
10.1186/1471-2164-11-90
language
English
UGent publication?
yes
classification
A1
additional info
article no. 90 (18 p.)
copyright statement
I have retained and own the full copyright for this publication
id
942010
handle
http://hdl.handle.net/1854/LU-942010
date created
2010-05-04 14:20:07
date last changed
2016-12-19 15:44:41
@article{942010,
  abstract     = {Background: Burkholderia cepacia complex bacteria are opportunistic pathogens, which can cause severe respiratory tract infections in patients with cystic fibrosis (CF). As treatment of infected CF patients is problematic, multiple preventive measures are taken to reduce the infection risk. Besides a stringent segregation policy to prevent patient-to-patient transmission, clinicians also advise patients to clean and disinfect their respiratory equipment on a regular basis. However, problems regarding the efficacy of several disinfection procedures for the removal and/or killing of B. cepacia complex bacteria have been reported. In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of reactive oxygen species (ROS), the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2 or NaOCl.
Results: The exposure to H2O2 and NaOCl resulted in an upregulation of the transcription of 315 (4.4\%) and 386 (5.4\%) genes, respectively. Transcription of 185 (2.6\%) and 331 (4.6\%) genes was decreased in response to the respective treatments. Many of the upregulated genes in the NaOCl- and H2O2-treated biofilms are involved in oxidative stress as well as general stress response, emphasizing the importance of the efficient neutralization and scavenging of ROS. In addition, multiple upregulated genes encode proteins that are necessary to repair ROS-induced cellular damage. Unexpectedly, a prolonged treatment with H2O2 also resulted in an increased transcription of multiple phage-related genes. A closer inspection of hybridisation signals obtained with probes targeting intergenic regions led to the identification of a putative 6S RNA.
Conclusion: Our results reveal that the transcription of a large fraction of B. cenocepacia J2315 genes is altered upon exposure of sessile cells to ROS. These observations have highlighted that B. cenocepacia may alter several pathways in response to exposure to ROS and they have led to the identification of many genes not previously implicated in the stress response of this pathogen.},
  author       = {Peeters, Elke and Sass, Andrea and Mahenthiralingam, Eshwar and Nelis, Hans and Coenye, Tom},
  issn         = {1471-2164},
  journal      = {BMC GENOMICS},
  keyword      = {CYSTIC-FIBROSIS PATIENTS,ALKYL HYDROPEROXIDE REDUCTASE,QUALITY-CONTROL MAQC,PSEUDOMONAS-AERUGINOSA,ESCHERICHIA-COLI,CEPACIA COMPLEX,OXIDATIVE STRESS,ORGANIC HYDROPEROXIDE,SIGMA-FACTOR,BIOFILM FORMATION},
  language     = {eng},
  title        = {Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite},
  url          = {http://dx.doi.org/10.1186/1471-2164-11-90},
  volume       = {11},
  year         = {2010},
}

Chicago
Peeters, Elke, Andrea Sass, Eshwar Mahenthiralingam, Hans Nelis, and Tom Coenye. 2010. “Transcriptional Response of Burkholderia Cenocepacia J2315 Sessile Cells to Treatments with High Doses of Hydrogen Peroxide and Sodium Hypochlorite.” Bmc Genomics 11.
APA
Peeters, Elke, Sass, A., Mahenthiralingam, E., Nelis, H., & Coenye, T. (2010). Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite. BMC GENOMICS, 11.
Vancouver
1.
Peeters E, Sass A, Mahenthiralingam E, Nelis H, Coenye T. Transcriptional response of Burkholderia cenocepacia J2315 sessile cells to treatments with high doses of hydrogen peroxide and sodium hypochlorite. BMC GENOMICS. 2010;11.
MLA
Peeters, Elke, Andrea Sass, Eshwar Mahenthiralingam, et al. “Transcriptional Response of Burkholderia Cenocepacia J2315 Sessile Cells to Treatments with High Doses of Hydrogen Peroxide and Sodium Hypochlorite.” BMC GENOMICS 11 (2010): n. pag. Print.