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Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments

(2010) EUPHYTICA. 171(2). p.283-293
Author
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Abstract
In this study, treatments of both trifluralin (at 10, 100 and 1000 mu M) and N2O (in the form of gas under pressure) were applied to Begonia flower buds to induce the formation of 2n pollen. Three male fertile species (B. cucullata, B. subvillosa var. leptotricha and B. fischeri) and two male sterile hybrids (B. schmidtiana x B. cucullata and B. subvillosa var. leptotricha x B. cucullata) were treated. Pollen size, which is related to pollen DNA content, increased after both N2O and trifluralin treatments, but the induction of large pollen was genotype dependent. Trifluralin induced large pollen only in the male fertile species, while N2O treatments induced fertile 2n pollen in the male sterile B. schmidtiana x B. cucullata. Cytological studies showed that trifluralin induced multinuclear monads that resulted in 4n gametes in stead of 2n gametes. In general, large pollen obtained after trifluralin treatments showed low germination capability, while large pollen obtained after N2O treatments retained high germination capability. Seedlings with raised ploidy level could only be obtained after crosses were performed with large pollen obtained from N2O treatments. Hence, N2O treatments are preferable to the use of trifluralin to induce 2n gametes in Begonia.
Keywords
Meiosis, Germination, Nuclei, Polyploids, Progeny, Unreduced gametes, NITROUS-OXIDE GAS, PLANT MORPHOLOGY, GENE-EXPRESSION, POLYPLOIDY, MAIZE, MECHANISMS, EVOLUTION, HYBRIDS, TETRAPLOIDY, ANGIOSPERMS

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Citation

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MLA
Dewitte, Angelo et al. “Induction of 2n Pollen Formation in Begonia by Trifluralin and N2O Treatments.” EUPHYTICA 171.2 (2010): 283–293. Print.
APA
Dewitte, A., Eeckhaut, T., Van Huylenbroeck, J., & Van Bockstaele, E. (2010). Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments. EUPHYTICA, 171(2), 283–293.
Chicago author-date
Dewitte, Angelo, Tom Eeckhaut, Johan Van Huylenbroeck, and Erik Van Bockstaele. 2010. “Induction of 2n Pollen Formation in Begonia by Trifluralin and N2O Treatments.” Euphytica 171 (2): 283–293.
Chicago author-date (all authors)
Dewitte, Angelo, Tom Eeckhaut, Johan Van Huylenbroeck, and Erik Van Bockstaele. 2010. “Induction of 2n Pollen Formation in Begonia by Trifluralin and N2O Treatments.” Euphytica 171 (2): 283–293.
Vancouver
1.
Dewitte A, Eeckhaut T, Van Huylenbroeck J, Van Bockstaele E. Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments. EUPHYTICA. 2010;171(2):283–93.
IEEE
[1]
A. Dewitte, T. Eeckhaut, J. Van Huylenbroeck, and E. Van Bockstaele, “Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments,” EUPHYTICA, vol. 171, no. 2, pp. 283–293, 2010.
@article{941309,
  abstract     = {In this study, treatments of both trifluralin (at 10, 100 and 1000 mu M) and N2O (in the form of gas under pressure) were applied to Begonia flower buds to induce the formation of 2n pollen. Three male fertile species (B. cucullata, B. subvillosa var. leptotricha and B. fischeri) and two male sterile hybrids (B. schmidtiana x B. cucullata and B. subvillosa var. leptotricha x B. cucullata) were treated. Pollen size, which is related to pollen DNA content, increased after both N2O and trifluralin treatments, but the induction of large pollen was genotype dependent. Trifluralin induced large pollen only in the male fertile species, while N2O treatments induced fertile 2n pollen in the male sterile B. schmidtiana x B. cucullata. Cytological studies showed that trifluralin induced multinuclear monads that resulted in 4n gametes in stead of 2n gametes. In general, large pollen obtained after trifluralin treatments showed low germination capability, while large pollen obtained after N2O treatments retained high germination capability. Seedlings with raised ploidy level could only be obtained after crosses were performed with large pollen obtained from N2O treatments. Hence, N2O treatments are preferable to the use of trifluralin to induce 2n gametes in Begonia.},
  author       = {Dewitte, Angelo and Eeckhaut, Tom and Van Huylenbroeck, Johan and Van Bockstaele, Erik},
  issn         = {0014-2336},
  journal      = {EUPHYTICA},
  keywords     = {Meiosis,Germination,Nuclei,Polyploids,Progeny,Unreduced gametes,NITROUS-OXIDE GAS,PLANT MORPHOLOGY,GENE-EXPRESSION,POLYPLOIDY,MAIZE,MECHANISMS,EVOLUTION,HYBRIDS,TETRAPLOIDY,ANGIOSPERMS},
  language     = {eng},
  number       = {2},
  pages        = {283--293},
  title        = {Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments},
  url          = {http://dx.doi.org/10.1007/s10681-009-0060-z},
  volume       = {171},
  year         = {2010},
}

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