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Meiotic aberrations during 2n pollen formation in Begonia

(2010) HEREDITY. 104(2). p.215-223
Author
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Abstract
Unreduced gametes are the driving force for the polyploidization of plants in nature, and are also an important tool for ploidy breeding. The final heterozygosity of a 2n pollen grain depends on the cytological mechanism behind 2n pollen formation. In this study, chromosome pairing and chromosome segregation during the microsporogenesis of seven Begonia genotypes were analysed using fluorescent chromosome staining on (squashed) pollen mother cells. Among the seven genotypes, five genotypes produce 2n pollen (B. 'Bubbles', B. 'Florence Rita', B. 'Orococo', B. 'Tamo' and B276) and two genotypes produce only normal n pollen (B. fischeri and B243). All 2n pollen producers showed a mechanism equivalent to first division restitution (FDR), in which chromosomes did not segregate during meiosis I but only during meiosis II. This FDR was the result of (a) an irregular chromosome pairing in B. 'Tamo', (b) stickiness of chromosomes associated with numerous chromosome bridges in B. 'Florence Rita' and B276, and (c) a combination of irregular chromosome pairing and stickiness of chromosomes in B. 'Bubbles'. The exact mechanism of the nuclear restitution in B. 'Orococo' could not be determined. Other mechanisms, such as early asymmetric cytokinesis, omission of meiosis II, parallel or tripolar spindle formation, were rather uncommon. Unpaired chromosomes (univalents) were observed in all genotypes, but they had moved to one of the poles by the end of anaphase I or II. Only B. 'Tamo' formed a high number of micronuclei. Consequently, this genotype formed a large number of malformed pollen. Obviously, chromosome behaviour during meiosis in Begonia is very dynamic, which may have important consequences for chromosome evolution and biodiversity within the genus. Heredity (2010) 104, 215-223; doi:10.1038/hdy.2009.111; published online 26 August 2009
Keywords
HYBRIDS, BRIDGE, RESTITUTION, CELL-LINE, CHROMOSOME INSTABILITY, SEXUAL POLYPLOIDIZATION, SPONTANEOUS TELOMERE LOSS, restitution, chromosome bridges, chromosome pairing, univalents, meiosis, polyploids, HETEROZYGOSITY, ORGANIZATION, SEGREGATION

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Citation

Please use this url to cite or link to this publication:

MLA
Dewitte, Angelo et al. “Meiotic Aberrations During 2n Pollen Formation in Begonia.” HEREDITY 104.2 (2010): 215–223. Print.
APA
Dewitte, A., Eeckhaut, T., Van Huylenbroeck, J., & Van Bockstaele, E. (2010). Meiotic aberrations during 2n pollen formation in Begonia. HEREDITY, 104(2), 215–223.
Chicago author-date
Dewitte, Angelo, Tom Eeckhaut, Johan Van Huylenbroeck, and Erik Van Bockstaele. 2010. “Meiotic Aberrations During 2n Pollen Formation in Begonia.” Heredity 104 (2): 215–223.
Chicago author-date (all authors)
Dewitte, Angelo, Tom Eeckhaut, Johan Van Huylenbroeck, and Erik Van Bockstaele. 2010. “Meiotic Aberrations During 2n Pollen Formation in Begonia.” Heredity 104 (2): 215–223.
Vancouver
1.
Dewitte A, Eeckhaut T, Van Huylenbroeck J, Van Bockstaele E. Meiotic aberrations during 2n pollen formation in Begonia. HEREDITY. 2010;104(2):215–23.
IEEE
[1]
A. Dewitte, T. Eeckhaut, J. Van Huylenbroeck, and E. Van Bockstaele, “Meiotic aberrations during 2n pollen formation in Begonia,” HEREDITY, vol. 104, no. 2, pp. 215–223, 2010.
@article{941306,
  abstract     = {Unreduced gametes are the driving force for the polyploidization of plants in nature, and are also an important tool for ploidy breeding. The final heterozygosity of a 2n pollen grain depends on the cytological mechanism behind 2n pollen formation. In this study, chromosome pairing and chromosome segregation during the microsporogenesis of seven Begonia genotypes were analysed using fluorescent chromosome staining on (squashed) pollen mother cells. Among the seven genotypes, five genotypes produce 2n pollen (B. 'Bubbles', B. 'Florence Rita', B. 'Orococo', B. 'Tamo' and B276) and two genotypes produce only normal n pollen (B. fischeri and B243). All 2n pollen producers showed a mechanism equivalent to first division restitution (FDR), in which chromosomes did not segregate during meiosis I but only during meiosis II. This FDR was the result of (a) an irregular chromosome pairing in B. 'Tamo', (b) stickiness of chromosomes associated with numerous chromosome bridges in B. 'Florence Rita' and B276, and (c) a combination of irregular chromosome pairing and stickiness of chromosomes in B. 'Bubbles'. The exact mechanism of the nuclear restitution in B. 'Orococo' could not be determined. Other mechanisms, such as early asymmetric cytokinesis, omission of meiosis II, parallel or tripolar spindle formation, were rather uncommon. Unpaired chromosomes (univalents) were observed in all genotypes, but they had moved to one of the poles by the end of anaphase I or II. Only B. 'Tamo' formed a high number of micronuclei. Consequently, this genotype formed a large number of malformed pollen. Obviously, chromosome behaviour during meiosis in Begonia is very dynamic, which may have important consequences for chromosome evolution and biodiversity within the genus. Heredity (2010) 104, 215-223; doi:10.1038/hdy.2009.111; published online 26 August 2009},
  author       = {Dewitte, Angelo and Eeckhaut, Tom and Van Huylenbroeck, Johan and Van Bockstaele, Erik},
  issn         = {0018-067X},
  journal      = {HEREDITY},
  keywords     = {HYBRIDS,BRIDGE,RESTITUTION,CELL-LINE,CHROMOSOME INSTABILITY,SEXUAL POLYPLOIDIZATION,SPONTANEOUS TELOMERE LOSS,restitution,chromosome bridges,chromosome pairing,univalents,meiosis,polyploids,HETEROZYGOSITY,ORGANIZATION,SEGREGATION},
  language     = {eng},
  number       = {2},
  pages        = {215--223},
  title        = {Meiotic aberrations during 2n pollen formation in Begonia},
  url          = {http://dx.doi.org/10.1038/hdy.2009.111},
  volume       = {104},
  year         = {2010},
}

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