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Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers

Ivan Meeus UGent, Dirk de Graaf UGent, K Jans and Guy Smagghe UGent (2010) JOURNAL OF APPLIED MICROBIOLOGY. 109(1). p.107-115
abstract
Aims: The aims of this study were to design universal markers for different protozoan parasites of Bombus spp. based on the phylogenetic position of two important bumblebee parasites Crithidia bombi and Apicystis bombi. Methods and Results: Standard PCR and extraction techniques were used to amplify and sequence 18S rDNA. Phylogenetic analysis of the rDNA was performed in order to predict the parasite range of the primers. Conclusions: Crithidia bombi phylogenetically clusters with the trypanosomatids with slowly-evolving SSU-rRNA sequences (SE), while A. bombi is the closest sister group of Mattesia. A multiplex was designed containing an internal control and two broad-range primer pairs, detecting C. bombi and other SE trypanosomatids and also A. bombi and other neogregarines. Significance and Impact of the Study: Sequence data generated will further improve the current systematics of insect trypanosomatids and gregarines that remain troublesome. Broad-range markers for bumblebee parasites are necessary tools enabling the screening of commercially imported colonies and thus controlling their worldwide distribution and to discover related emerging parasites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Apicystis bombi, 18S rDNA, Crithidia bombi, phylogeny, protozoa detection, BEES BOMBUS, COLONY COLLAPSE, PARASITIC MITES, NOSEMA-CERANAE, HYMENOPTERA, APIDAE, DECLINE, POLLINATION, PHYLOGENY, CRITHIDIA
journal title
JOURNAL OF APPLIED MICROBIOLOGY
J. Appl. Microbiol.
volume
109
issue
1
pages
107 - 115
Web of Science type
Article
Web of Science id
000278674300010
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
2.365 (2010)
JCR rank
64/158 (2010)
JCR quartile
2 (2010)
ISSN
1364-5072
DOI
10.1111/j.1365-2672.2009.04635.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
935582
handle
http://hdl.handle.net/1854/LU-935582
date created
2010-04-22 23:24:34
date last changed
2011-01-18 13:59:55
@article{935582,
  abstract     = {Aims: The aims of this study were to design universal markers for different protozoan parasites of Bombus spp. based on the phylogenetic position of two important bumblebee parasites Crithidia bombi and Apicystis bombi.
Methods and Results: Standard PCR and extraction techniques were used to amplify and sequence 18S rDNA. Phylogenetic analysis of the rDNA was performed in order to predict the parasite range of the primers.
Conclusions: Crithidia bombi phylogenetically clusters with the trypanosomatids with slowly-evolving SSU-rRNA sequences (SE), while A. bombi is the closest sister group of Mattesia. A multiplex was designed containing an internal control and two broad-range primer pairs, detecting C. bombi and other SE trypanosomatids and also A. bombi and other neogregarines.
Significance and Impact of the Study: Sequence data generated will further improve the current systematics of insect trypanosomatids and gregarines that remain troublesome. Broad-range markers for bumblebee parasites are necessary tools enabling the screening of commercially imported colonies and thus controlling their worldwide distribution and to discover related emerging parasites.},
  author       = {Meeus, Ivan and de Graaf, Dirk and Jans, K and Smagghe, Guy},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keyword      = {Apicystis bombi,18S rDNA,Crithidia bombi,phylogeny,protozoa detection,BEES BOMBUS,COLONY COLLAPSE,PARASITIC MITES,NOSEMA-CERANAE,HYMENOPTERA,APIDAE,DECLINE,POLLINATION,PHYLOGENY,CRITHIDIA},
  language     = {eng},
  number       = {1},
  pages        = {107--115},
  title        = {Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2009.04635.x},
  volume       = {109},
  year         = {2010},
}

Chicago
Meeus, Ivan, Dirk de Graaf, K Jans, and Guy Smagghe. 2010. “Multiplex PCR Detection of Slowly-evolving Trypanosomatids and Neogregarines in Bumblebees Using Broad-range Primers.” Journal of Applied Microbiology 109 (1): 107–115.
APA
Meeus, I., de Graaf, D., Jans, K., & Smagghe, G. (2010). Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers. JOURNAL OF APPLIED MICROBIOLOGY, 109(1), 107–115.
Vancouver
1.
Meeus I, de Graaf D, Jans K, Smagghe G. Multiplex PCR detection of slowly-evolving trypanosomatids and neogregarines in bumblebees using broad-range primers. JOURNAL OF APPLIED MICROBIOLOGY. 2010;109(1):107–15.
MLA
Meeus, Ivan, Dirk de Graaf, K Jans, et al. “Multiplex PCR Detection of Slowly-evolving Trypanosomatids and Neogregarines in Bumblebees Using Broad-range Primers.” JOURNAL OF APPLIED MICROBIOLOGY 109.1 (2010): 107–115. Print.