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Effective treatment of respiratory alphaherpesvirus infection using RNA interference

Amy Fulton, Sarah T Peters, Gillian A Perkins, Keith W Jarosinski, Armando Damiani, Margaret Brosnahan, Elizabeth L Buckles, Nikolaus Osterrieder and Gerlinde Van de Walle UGent (2009) PLOS ONE. 4(1).
abstract
Background: Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesvirinae, is spread via nasal secretions and causes respiratory disease, neurological disorders and abortions. The virus is a significant equine pathogen, but current EHV-1 vaccines are only partially protective and effective metaphylactic and therapeutic agents are not available. Small interfering RNAs (siRNA's), delivered intranasally, could prove a valuable alternative for infection control. siRNA's against two essential EHV-1 genes, encoding the viral helicase (Ori) and glycoprotein B, were evaluated for their potential to decrease EHV-1 infection in a mouse model. Methodology/Principal Fndings: siRNA therapy in vitro significantly reduced virus production and plaque size. Viral titers were reduced 80-fold with 37.5 pmol of a single siRNA or with as little as 6.25 pmol of each siRNA when used in combination. siRNA therapy in vivo significantly reduced viral replication and clinical signs. Intranasal treatment did not require a transport vehicle and proved effective when given up to 12 h before or after infection. Conclusions/Significance: siRNA treatment has potential for both prevention and early treatment of EHV-1 infections.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
PLOS ONE
PLoS One
volume
4
issue
1
Web of Science type
Article
Web of Science id
000265472900003
JCR category
BIOLOGY
JCR impact factor
4.351 (2009)
JCR rank
9/73 (2009)
JCR quartile
1 (2009)
ISSN
1932-6203
DOI
10.1371/journal.pone.0004118
language
English
UGent publication?
no
classification
A1
additional info
article no. e4118 (10 p.)
copyright statement
I have retained and own the full copyright for this publication
id
933911
handle
http://hdl.handle.net/1854/LU-933911
date created
2010-04-21 10:12:46
date last changed
2010-04-29 14:34:30
@article{933911,
  abstract     = {Background: Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesvirinae, is spread via nasal secretions and causes respiratory disease, neurological disorders and abortions. The virus is a significant equine pathogen, but current EHV-1 vaccines are only partially protective and effective metaphylactic and therapeutic agents are not available. Small interfering RNAs (siRNA's), delivered intranasally, could prove a valuable alternative for infection control. siRNA's against two essential EHV-1 genes, encoding the viral helicase (Ori) and glycoprotein B, were evaluated for their potential to decrease EHV-1 infection in a mouse model.
Methodology/Principal Fndings: siRNA therapy in vitro significantly reduced virus production and plaque size. Viral titers were reduced 80-fold with 37.5 pmol of a single siRNA or with as little as 6.25 pmol of each siRNA when used in combination. siRNA therapy in vivo significantly reduced viral replication and clinical signs. Intranasal treatment did not require a transport vehicle and proved effective when given up to 12 h before or after infection.
Conclusions/Significance: siRNA treatment has potential for both prevention and early treatment of EHV-1 infections.},
  author       = {Fulton, Amy and Peters, Sarah T and Perkins, Gillian A and Jarosinski, Keith W and Damiani, Armando and Brosnahan, Margaret and Buckles, Elizabeth L and Osterrieder, Nikolaus and Van de Walle, Gerlinde},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {1},
  title        = {Effective treatment of respiratory alphaherpesvirus infection using RNA interference},
  url          = {http://dx.doi.org/10.1371/journal.pone.0004118},
  volume       = {4},
  year         = {2009},
}

Chicago
Fulton, Amy, Sarah T Peters, Gillian A Perkins, Keith W Jarosinski, Armando Damiani, Margaret Brosnahan, Elizabeth L Buckles, Nikolaus Osterrieder, and Gerlinde Van de Walle. 2009. “Effective Treatment of Respiratory Alphaherpesvirus Infection Using RNA Interference.” Plos One 4 (1).
APA
Fulton, A., Peters, S. T., Perkins, G. A., Jarosinski, K. W., Damiani, A., Brosnahan, M., Buckles, E. L., et al. (2009). Effective treatment of respiratory alphaherpesvirus infection using RNA interference. PLOS ONE, 4(1).
Vancouver
1.
Fulton A, Peters ST, Perkins GA, Jarosinski KW, Damiani A, Brosnahan M, et al. Effective treatment of respiratory alphaherpesvirus infection using RNA interference. PLOS ONE. 2009;4(1).
MLA
Fulton, Amy, Sarah T Peters, Gillian A Perkins, et al. “Effective Treatment of Respiratory Alphaherpesvirus Infection Using RNA Interference.” PLOS ONE 4.1 (2009): n. pag. Print.