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The ability of bovine mammary tissue to synthesize lipids for 96-H when cocultured with liver and adipose-tissue

Author
Organization
Abstract
Explants of bovine mammary, liver, and adipose tissues were cocultured in the same well for 24, 48, 72 or 96 h and their ability to synthesize lipids was measured by the incorporation of [C-14]acetate into triglycerides, free fatty acids, and phospholipids. The model was developed to study the effect of bovine somatotropin on lipid synthesis in mammary tissue, because somatotropin will not affect mammary tissue unless liver tissue is present. Somatotropin reduced incorporation by mammary tissue relative to the control. However, the rate of incorporation remained constant through % h with somatotropin, but steadily decreased in control mammary tissue. Phospholipid release into the media increased dramatically from 2% of that synthesized during the first 24 h to 218% of that synthesized from 72 to 96 h. The high release of phospholipids after 24 h suggests that cellular breakdown was occurring. A 24-h incubation is recommended to study lipid synthesis with this system.
Keywords
LIPID SYNTHESIS, BOVINE SOMATOTROPIN, TRIGLYCERIDES, FREE FATTY ACIDS, PHOSPHOLIPIDS, GROWTH-HORMONE, INSULIN, RECEPTORS, PROLACTIN, BINDING, GLAND, COWS

Citation

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MLA
KEYS, JE et al. “The Ability of Bovine Mammary Tissue to Synthesize Lipids for 96-H When Cocultured with Liver and Adipose-tissue.” BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 70.5 (1992): 343–346. Print.
APA
KEYS, J., FEKRY, A., WOOD, D., & Capuco, A. (1992). The ability of bovine mammary tissue to synthesize lipids for 96-H when cocultured with liver and adipose-tissue. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 70(5), 343–346.
Chicago author-date
KEYS, JE, AE FEKRY, DL WOOD, and Anthony Capuco. 1992. “The Ability of Bovine Mammary Tissue to Synthesize Lipids for 96-H When Cocultured with Liver and Adipose-tissue.” Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire 70 (5): 343–346.
Chicago author-date (all authors)
KEYS, JE, AE FEKRY, DL WOOD, and Anthony Capuco. 1992. “The Ability of Bovine Mammary Tissue to Synthesize Lipids for 96-H When Cocultured with Liver and Adipose-tissue.” Biochemistry and Cell Biology-biochimie Et Biologie Cellulaire 70 (5): 343–346.
Vancouver
1.
KEYS J, FEKRY A, WOOD D, Capuco A. The ability of bovine mammary tissue to synthesize lipids for 96-H when cocultured with liver and adipose-tissue. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE. 1992;70(5):343–6.
IEEE
[1]
J. KEYS, A. FEKRY, D. WOOD, and A. Capuco, “The ability of bovine mammary tissue to synthesize lipids for 96-H when cocultured with liver and adipose-tissue,” BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, vol. 70, no. 5, pp. 343–346, 1992.
@article{932442,
  abstract     = {Explants of bovine mammary, liver, and adipose tissues were cocultured in the same well for 24, 48, 72 or 96 h and their ability to synthesize lipids was measured by the incorporation of [C-14]acetate into triglycerides, free fatty acids, and phospholipids. The model was developed to study the effect of bovine somatotropin on lipid synthesis in mammary tissue, because somatotropin will not affect mammary tissue unless liver tissue is present. Somatotropin reduced incorporation by mammary tissue relative to the control. However, the rate of incorporation remained constant through % h with somatotropin, but steadily decreased in control mammary tissue. Phospholipid release into the media increased dramatically from 2% of that synthesized during the first 24 h to 218% of that synthesized from 72 to 96 h. The high release of phospholipids after 24 h suggests that cellular breakdown was occurring. A 24-h incubation is recommended to study lipid synthesis with this system.},
  author       = {KEYS, JE and FEKRY, AE and WOOD, DL and Capuco, Anthony},
  issn         = {0829-8211},
  journal      = {BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE},
  keywords     = {LIPID SYNTHESIS,BOVINE SOMATOTROPIN,TRIGLYCERIDES,FREE FATTY ACIDS,PHOSPHOLIPIDS,GROWTH-HORMONE,INSULIN,RECEPTORS,PROLACTIN,BINDING,GLAND,COWS},
  language     = {eng},
  number       = {5},
  pages        = {343--346},
  title        = {The ability of bovine mammary tissue to synthesize lipids for 96-H when cocultured with liver and adipose-tissue},
  volume       = {70},
  year         = {1992},
}