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Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signaling in the pea aphid

Olivier Christiaens UGent, Masatoshi Iga UGent, RA Velarde, Pierre Rougé and Guy Smagghe UGent (2010) INSECT MOLECULAR BIOLOGY. 19(suppl. 2). p.187-200
abstract
The pea aphid (Acyrthosiphon pisum) is the first whole genome sequenced insect with a hemimetabolic development and an emerging model organism for studies in ecology, evolution and development. The insect steroid moulting hormone 20-hydroxyecdysone (20E) controls and coordinates development in insects, especially the moulting/metamorphosis process. We, therefore present here a comprehensive characterization of the Halloween genes phantom, disembodied, shadow, shade, spook and spookiest, coding for the P450 enzymes that control the biosynthesis of 20E. Regarding the presence of nuclear receptors in the pea aphid genome, we found 19 genes, representing all of the seven known subfamilies. The annotation and phylogenetic analysis revealed a strong conservation in the class of Insecta. But compared with other sequenced insect genomes, three orthologues are missing in the Acyrthosiphon genome, namely HR96, PNR-like and Knirps. We also cloned the EcR, Usp, E75 and HR3. Finally, 3D-modelling of the ligand-binding domain of Ap-EcR exhibited the typical canonical structural scaffold with 12 alpha-helices associated with a short hairpin of two antiparallel beta-strands. Upon docking, 20E was located in the hormone-binding groove, supporting the hypothesis that EcR has a role in 20E signalling.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
nuclear receptors, ecdysteroid biosynthesis, ecdysteroid cascade, pea aphid, development, moulting, metamorphosis, LIGAND-BINDING POCKET, DROSOPHILA-MELANOGASTER, ECDYSONE BIOSYNTHESIS, HORMONE-RECEPTOR, MOLTING HORMONE, BOMBYX-MORI, DEVELOPMENTAL EXPRESSION, CYTOCHROME-P450 ENZYME, GENOME SEQUENCE, ORPHAN RECEPTOR
journal title
INSECT MOLECULAR BIOLOGY
Insect Mol. Biol.
volume
19
issue
suppl. 2
pages
187 - 200
Web of Science type
Article
Web of Science id
000274909200018
JCR category
ENTOMOLOGY
JCR impact factor
2.669 (2010)
JCR rank
5/83 (2010)
JCR quartile
1 (2010)
ISSN
0962-1075
DOI
10.1111/j.1365-2583.2009.00957.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
930917
handle
http://hdl.handle.net/1854/LU-930917
date created
2010-04-17 11:43:14
date last changed
2010-04-23 11:40:15
@article{930917,
  abstract     = {The pea aphid (Acyrthosiphon pisum) is the first whole genome sequenced insect with a hemimetabolic development and an emerging model organism for studies in ecology, evolution and development. The insect steroid moulting hormone 20-hydroxyecdysone (20E) controls and coordinates development in insects, especially the moulting/metamorphosis process. We, therefore present here a comprehensive characterization of the Halloween genes phantom, disembodied, shadow, shade, spook and spookiest, coding for the P450 enzymes that control the biosynthesis of 20E. Regarding the presence of nuclear receptors in the pea aphid genome, we found 19 genes, representing all of the seven known subfamilies. The annotation and phylogenetic analysis revealed a strong conservation in the class of Insecta. But compared with other sequenced insect genomes, three orthologues are missing in the Acyrthosiphon genome, namely HR96, PNR-like and Knirps. We also cloned the EcR, Usp, E75 and HR3. Finally, 3D-modelling of the ligand-binding domain of Ap-EcR exhibited the typical canonical structural scaffold with 12 alpha-helices associated with a short hairpin of two antiparallel beta-strands. Upon docking, 20E was located in the hormone-binding groove, supporting the hypothesis that EcR has a role in 20E signalling.},
  author       = {Christiaens, Olivier and Iga, Masatoshi and Velarde, RA and Roug{\'e}, Pierre and Smagghe, Guy},
  issn         = {0962-1075},
  journal      = {INSECT MOLECULAR BIOLOGY},
  keyword      = {nuclear receptors,ecdysteroid biosynthesis,ecdysteroid cascade,pea aphid,development,moulting,metamorphosis,LIGAND-BINDING POCKET,DROSOPHILA-MELANOGASTER,ECDYSONE BIOSYNTHESIS,HORMONE-RECEPTOR,MOLTING HORMONE,BOMBYX-MORI,DEVELOPMENTAL EXPRESSION,CYTOCHROME-P450 ENZYME,GENOME SEQUENCE,ORPHAN RECEPTOR},
  language     = {eng},
  number       = {suppl. 2},
  pages        = {187--200},
  title        = {Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signaling in the pea aphid},
  url          = {http://dx.doi.org/10.1111/j.1365-2583.2009.00957.x},
  volume       = {19},
  year         = {2010},
}

Chicago
Christiaens, Olivier, Masatoshi Iga, RA Velarde, Pierre Rougé, and Guy Smagghe. 2010. “Halloween Genes and Nuclear Receptors in Ecdysteroid Biosynthesis and Signaling in the Pea Aphid.” Insect Molecular Biology 19 (suppl. 2): 187–200.
APA
Christiaens, O., Iga, M., Velarde, R., Rougé, P., & Smagghe, G. (2010). Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signaling in the pea aphid. INSECT MOLECULAR BIOLOGY, 19(suppl. 2), 187–200.
Vancouver
1.
Christiaens O, Iga M, Velarde R, Rougé P, Smagghe G. Halloween genes and nuclear receptors in ecdysteroid biosynthesis and signaling in the pea aphid. INSECT MOLECULAR BIOLOGY. 2010;19(suppl. 2):187–200.
MLA
Christiaens, Olivier, Masatoshi Iga, RA Velarde, et al. “Halloween Genes and Nuclear Receptors in Ecdysteroid Biosynthesis and Signaling in the Pea Aphid.” INSECT MOLECULAR BIOLOGY 19.suppl. 2 (2010): 187–200. Print.