Advanced search
1 file | 456.32 KB Add to list

NINJA connects the co-repressor TOPLESS to jasmonate signalling

(2010) NATURE. 464(7289). p.788-791
Author
Organization
Abstract
Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.
Keywords
DEFENSE, ARABIDOPSIS-THALIANA, TANDEM AFFINITY PURIFICATION, FUNCTIONAL GENOMICS, STRESS-RESPONSE, PROTEIN FAMILY, DEGRADATION, AUXIN, CELLS, GENES

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 456.32 KB

Citation

Please use this url to cite or link to this publication:

MLA
Pauwels, Laurens et al. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” NATURE 464.7289 (2010): 788–791. Print.
APA
Pauwels, Laurens, Fernández Barbero, G., Geerinck, J., Tilleman, S., Grunewald, W., Cuéllar Pérez, A., Chico, J. M., et al. (2010). NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE, 464(7289), 788–791.
Chicago author-date
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, Sofie Tilleman, Wim Grunewald, Amparo Cuéllar Pérez, José Manuel Chico, et al. 2010. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” Nature 464 (7289): 788–791.
Chicago author-date (all authors)
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, Sofie Tilleman, Wim Grunewald, Amparo Cuéllar Pérez, José Manuel Chico, Robin Vanden Bossche, Jared Sewell, Eduardo Gil, Gloria Garcia-Casado, Erwin Witters, Dirk Inzé, Jeff A Long, Geert De Jaeger, Roberto Solano, and Alain Goossens. 2010. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” Nature 464 (7289): 788–791.
Vancouver
1.
Pauwels L, Fernández Barbero G, Geerinck J, Tilleman S, Grunewald W, Cuéllar Pérez A, et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE. 2010;464(7289):788–91.
IEEE
[1]
L. Pauwels et al., “NINJA connects the co-repressor TOPLESS to jasmonate signalling,” NATURE, vol. 464, no. 7289, pp. 788–791, 2010.
@article{924840,
  abstract     = {Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.},
  author       = {Pauwels, Laurens and Fernández Barbero, Gemma and Geerinck, Jan and Tilleman, Sofie and Grunewald, Wim and Cuéllar Pérez, Amparo and Chico, José Manuel and Vanden Bossche, Robin and Sewell, Jared and Gil, Eduardo and Garcia-Casado, Gloria and Witters, Erwin and Inzé, Dirk and Long, Jeff A and De Jaeger, Geert and Solano, Roberto and Goossens, Alain},
  issn         = {0028-0836},
  journal      = {NATURE},
  keywords     = {DEFENSE,ARABIDOPSIS-THALIANA,TANDEM AFFINITY PURIFICATION,FUNCTIONAL GENOMICS,STRESS-RESPONSE,PROTEIN FAMILY,DEGRADATION,AUXIN,CELLS,GENES},
  language     = {eng},
  number       = {7289},
  pages        = {788--791},
  title        = {NINJA connects the co-repressor TOPLESS to jasmonate signalling},
  url          = {http://dx.doi.org/10.1038/nature08854},
  volume       = {464},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: