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NINJA connects the co-repressor TOPLESS to jasmonate signalling

Laurens Pauwels UGent, Gemma Fernández Barbero, Jan Geerinck UGent, Sofie Tilleman UGent, Wim Grunewald UGent, Amparo Cuéllar Pérez UGent, José Manuel Chico, Robin Vanden Bossche UGent, Jared Sewell and Eduardo Gil, et al. (2010) NATURE. 464(7289). p.788-791
abstract
Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DEFENSE, ARABIDOPSIS-THALIANA, TANDEM AFFINITY PURIFICATION, FUNCTIONAL GENOMICS, STRESS-RESPONSE, PROTEIN FAMILY, DEGRADATION, AUXIN, CELLS, GENES
journal title
NATURE
Nature
volume
464
issue
7289
pages
6 pages
Web of Science type
Article
Web of Science id
000276205000051
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
36.101 (2010)
JCR rank
1/56 (2010)
JCR quartile
1 (2010)
ISSN
0028-0836
DOI
10.1038/nature08854
language
English
UGent publication?
yes
classification
A1
additional info
online supplement: full methods
copyright statement
I have transferred the copyright for this publication to the publisher
id
924840
handle
http://hdl.handle.net/1854/LU-924840
date created
2010-04-13 16:51:26
date last changed
2012-09-19 14:03:56
@article{924840,
  abstract     = {Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.},
  author       = {Pauwels, Laurens and Fern{\'a}ndez Barbero, Gemma and Geerinck, Jan and Tilleman, Sofie and Grunewald, Wim and Cu{\'e}llar P{\'e}rez, Amparo and Chico, Jos{\'e} Manuel and Vanden Bossche, Robin and Sewell, Jared and Gil, Eduardo and Garcia-Casado, Gloria and Witters, Erwin and Inz{\'e}, Dirk and Long, Jeff A and De Jaeger, Geert and Solano, Roberto and Goossens, Alain},
  issn         = {0028-0836},
  journal      = {NATURE},
  keyword      = {DEFENSE,ARABIDOPSIS-THALIANA,TANDEM AFFINITY PURIFICATION,FUNCTIONAL GENOMICS,STRESS-RESPONSE,PROTEIN FAMILY,DEGRADATION,AUXIN,CELLS,GENES},
  language     = {eng},
  number       = {7289},
  pages        = {788--791},
  title        = {NINJA connects the co-repressor TOPLESS to jasmonate signalling},
  url          = {http://dx.doi.org/10.1038/nature08854},
  volume       = {464},
  year         = {2010},
}

Chicago
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, Sofie Tilleman, Wim Grunewald, Amparo Cuéllar Pérez, José Manuel Chico, et al. 2010. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” Nature 464 (7289): 788–791.
APA
Pauwels, Laurens, Fernández Barbero, G., Geerinck, J., Tilleman, S., Grunewald, W., Cuéllar Pérez, A., Chico, J. M., et al. (2010). NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE, 464(7289), 788–791.
Vancouver
1.
Pauwels L, Fernández Barbero G, Geerinck J, Tilleman S, Grunewald W, Cuéllar Pérez A, et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE. 2010;464(7289):788–91.
MLA
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, et al. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” NATURE 464.7289 (2010): 788–791. Print.