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NINJA connects the co-repressor TOPLESS to jasmonate signalling

(2010) NATURE. 464(7289). p.788-791
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Abstract
Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.
Keywords
DEFENSE, ARABIDOPSIS-THALIANA, TANDEM AFFINITY PURIFICATION, FUNCTIONAL GENOMICS, STRESS-RESPONSE, PROTEIN FAMILY, DEGRADATION, AUXIN, CELLS, GENES

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Chicago
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, Sofie Tilleman, Wim Grunewald, Amparo Cuéllar Pérez, José Manuel Chico, et al. 2010. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” Nature 464 (7289): 788–791.
APA
Pauwels, Laurens, Fernández Barbero, G., Geerinck, J., Tilleman, S., Grunewald, W., Cuéllar Pérez, A., Chico, J. M., et al. (2010). NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE, 464(7289), 788–791.
Vancouver
1.
Pauwels L, Fernández Barbero G, Geerinck J, Tilleman S, Grunewald W, Cuéllar Pérez A, et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling. NATURE. 2010;464(7289):788–91.
MLA
Pauwels, Laurens, Gemma Fernández Barbero, Jan Geerinck, et al. “NINJA Connects the Co-repressor TOPLESS to Jasmonate Signalling.” NATURE 464.7289 (2010): 788–791. Print.
@article{924840,
  abstract     = {Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.},
  author       = {Pauwels, Laurens and Fern{\'a}ndez Barbero, Gemma and Geerinck, Jan and Tilleman, Sofie and Grunewald, Wim and Cu{\'e}llar P{\'e}rez, Amparo and Chico, Jos{\'e} Manuel and Vanden Bossche, Robin and Sewell, Jared and Gil, Eduardo and Garcia-Casado, Gloria and Witters, Erwin and Inz{\'e}, Dirk and Long, Jeff A and De Jaeger, Geert and Solano, Roberto and Goossens, Alain},
  issn         = {0028-0836},
  journal      = {NATURE},
  language     = {eng},
  number       = {7289},
  pages        = {788--791},
  title        = {NINJA connects the co-repressor TOPLESS to jasmonate signalling},
  url          = {http://dx.doi.org/10.1038/nature08854},
  volume       = {464},
  year         = {2010},
}

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