Advanced search
1 file | 256.54 KB

GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro

Sarah Costers (UGent) , David Lefebvre (UGent) , Jan Van Doorsselaere (UGent) , Merijn Vanhee (UGent) , Peter Delputte (UGent) and Hans Nauwynck (UGent)
(2010) ARCHIVES OF VIROLOGY. 155(3). p.371-378
Author
Organization
Abstract
Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.
Keywords
LELYSTAD-VIRUS, STRUCTURAL PROTEIN, READING FRAMES 3, DEHYDROGENASE-ELEVATING VIRUS, SYNDROME PRRS VIRUS, INFECTION, GLYCOPROTEIN, IDENTIFICATION, MONOCLONAL-ANTIBODIES, PIGS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 256.54 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Costers, Sarah, David Lefebvre, Jan Van Doorsselaere, Merijn Vanhee, Peter Delputte, and Hans Nauwynck. 2010. “GP4 of Porcine Reproductive and Respiratory Syndrome Virus Contains a Neutralizing Epitope That Is Susceptible to Immunoselection in Vitro.” Archives of Virology 155 (3): 371–378.
APA
Costers, S., Lefebvre, D., Van Doorsselaere, J., Vanhee, M., Delputte, P., & Nauwynck, H. (2010). GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. ARCHIVES OF VIROLOGY, 155(3), 371–378.
Vancouver
1.
Costers S, Lefebvre D, Van Doorsselaere J, Vanhee M, Delputte P, Nauwynck H. GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. ARCHIVES OF VIROLOGY. 2010;155(3):371–8.
MLA
Costers, Sarah, David Lefebvre, Jan Van Doorsselaere, et al. “GP4 of Porcine Reproductive and Respiratory Syndrome Virus Contains a Neutralizing Epitope That Is Susceptible to Immunoselection in Vitro.” ARCHIVES OF VIROLOGY 155.3 (2010): 371–378. Print.
@article{924572,
  abstract     = {Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.},
  author       = {Costers, Sarah and Lefebvre, David and Van Doorsselaere, Jan and Vanhee, Merijn and Delputte, Peter and Nauwynck, Hans},
  issn         = {0304-8608},
  journal      = {ARCHIVES OF VIROLOGY},
  keyword      = {LELYSTAD-VIRUS,STRUCTURAL PROTEIN,READING FRAMES 3,DEHYDROGENASE-ELEVATING VIRUS,SYNDROME PRRS VIRUS,INFECTION,GLYCOPROTEIN,IDENTIFICATION,MONOCLONAL-ANTIBODIES,PIGS},
  language     = {eng},
  number       = {3},
  pages        = {371--378},
  title        = {GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro},
  url          = {http://dx.doi.org/10.1007/s00705-009-0582-7},
  volume       = {155},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: