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GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro

Sarah Costers UGent, David Lefebvre UGent, Jan Van Doorsselaere UGent, Merijn Vanhee UGent, Peter Delputte and Hans Nauwynck UGent (2010) ARCHIVES OF VIROLOGY. 155(3). p.371-378
abstract
Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LELYSTAD-VIRUS, STRUCTURAL PROTEIN, READING FRAMES 3, DEHYDROGENASE-ELEVATING VIRUS, SYNDROME PRRS VIRUS, INFECTION, GLYCOPROTEIN, IDENTIFICATION, MONOCLONAL-ANTIBODIES, PIGS
journal title
ARCHIVES OF VIROLOGY
Arch. Virol.
volume
155
issue
3
pages
8 pages
Web of Science type
Article
Web of Science id
000274939600010
JCR category
VIROLOGY
JCR impact factor
2.209 (2010)
JCR rank
19/32 (2010)
JCR quartile
3 (2010)
ISSN
0304-8608
DOI
10.1007/s00705-009-0582-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
924572
handle
http://hdl.handle.net/1854/LU-924572
date created
2010-04-13 15:01:04
date last changed
2016-12-19 15:46:25
@article{924572,
  abstract     = {Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.},
  author       = {Costers, Sarah and Lefebvre, David and Van Doorsselaere, Jan and Vanhee, Merijn and Delputte, Peter and Nauwynck, Hans},
  issn         = {0304-8608},
  journal      = {ARCHIVES OF VIROLOGY},
  keyword      = {LELYSTAD-VIRUS,STRUCTURAL PROTEIN,READING FRAMES 3,DEHYDROGENASE-ELEVATING VIRUS,SYNDROME PRRS VIRUS,INFECTION,GLYCOPROTEIN,IDENTIFICATION,MONOCLONAL-ANTIBODIES,PIGS},
  language     = {eng},
  number       = {3},
  pages        = {371--378},
  title        = {GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro},
  url          = {http://dx.doi.org/10.1007/s00705-009-0582-7},
  volume       = {155},
  year         = {2010},
}

Chicago
Costers, Sarah, David Lefebvre, Jan Van Doorsselaere, Merijn Vanhee, Peter Delputte, and Hans Nauwynck. 2010. “GP4 of Porcine Reproductive and Respiratory Syndrome Virus Contains a Neutralizing Epitope That Is Susceptible to Immunoselection in Vitro.” Archives of Virology 155 (3): 371–378.
APA
Costers, S., Lefebvre, D., Van Doorsselaere, J., Vanhee, M., Delputte, P., & Nauwynck, H. (2010). GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. ARCHIVES OF VIROLOGY, 155(3), 371–378.
Vancouver
1.
Costers S, Lefebvre D, Van Doorsselaere J, Vanhee M, Delputte P, Nauwynck H. GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro. ARCHIVES OF VIROLOGY. 2010;155(3):371–8.
MLA
Costers, Sarah, David Lefebvre, Jan Van Doorsselaere, et al. “GP4 of Porcine Reproductive and Respiratory Syndrome Virus Contains a Neutralizing Epitope That Is Susceptible to Immunoselection in Vitro.” ARCHIVES OF VIROLOGY 155.3 (2010): 371–378. Print.