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Green hydrophilic interaction chromatography using ethanol-water-carbon dioxide mixtures

(2010) JOURNAL OF SEPARATION SCIENCE. 33(6-7). p.834-837
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Abstract
In hydrophilic interaction chromatography (HILIC), best results are obtained with high concentrations of acetonitrile. In the framework of green chromatography, different concentrations of carbon dioxide were added to the mobile phases acetonitrile-water and ethanol-water and the impact on retention and separation in HILIC using bare silica as stationary phase was explored. The features of HILIC using enhanced-fluidity mobile phases are illustrated with the analysis of the nucleobases and a mixture containing the nucleobases and cortisol, flurbiprofen, theophylline and caffeine. For both organic constituents, the elution window is widened in function of the carbon dioxide concentration and selectivity changes. At high concentrations of carbon dioxide in ethanol, separations were similar to those obtained with acetonitrile without carbon dioxide addition.
Keywords
Hydrophilic interaction chromatography, PERFORMANCE LIQUID-CHROMATOGRAPHY, Green chromatography, Bare silica, Enhanced-fluidity mobile phases, ENHANCED-FLUIDITY, MOBILE PHASES, TEMPERATURE, SEPARATION

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Chicago
Dos Santos Pereira, Alberto, Ana Maria Jimenez Giron, Engdawork Admasu Engda, and Patrick Sandra. 2010. “Green Hydrophilic Interaction Chromatography Using Ethanol-water-carbon Dioxide Mixtures.” Ed. Michael Lämmerhofer. Journal of Separation Science 33 (6-7): 834–837.
APA
Dos Santos Pereira, A., Jimenez Giron, A. M., Admasu Engda, E., & Sandra, P. (2010). Green hydrophilic interaction chromatography using ethanol-water-carbon dioxide mixtures. (M. Lämmerhofer, Ed.)JOURNAL OF SEPARATION SCIENCE, 33(6-7), 834–837.
Vancouver
1.
Dos Santos Pereira A, Jimenez Giron AM, Admasu Engda E, Sandra P. Green hydrophilic interaction chromatography using ethanol-water-carbon dioxide mixtures. Lämmerhofer M, editor. JOURNAL OF SEPARATION SCIENCE. 2010;33(6-7):834–7.
MLA
Dos Santos Pereira, Alberto et al. “Green Hydrophilic Interaction Chromatography Using Ethanol-water-carbon Dioxide Mixtures.” Ed. Michael Lämmerhofer. JOURNAL OF SEPARATION SCIENCE 33.6-7 (2010): 834–837. Print.
@article{923024,
  abstract     = {In hydrophilic interaction chromatography (HILIC), best results are obtained with high concentrations of acetonitrile. In the framework of green chromatography, different concentrations of carbon dioxide were added to the mobile phases acetonitrile-water and ethanol-water and the impact on retention and separation in HILIC using bare silica as stationary phase was explored. The features of HILIC using enhanced-fluidity mobile phases are illustrated with the analysis of the nucleobases and a mixture containing the nucleobases and cortisol, flurbiprofen, theophylline and caffeine. For both organic constituents, the elution window is widened in function of the carbon dioxide concentration and selectivity changes. At high concentrations of carbon dioxide in ethanol, separations were similar to those obtained with acetonitrile without carbon dioxide addition.},
  author       = {Dos Santos Pereira, Alberto and Jimenez Giron, Ana Maria and Admasu Engda, Engdawork and Sandra, Patrick},
  editor       = {L{\"a}mmerhofer, Michael },
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  language     = {eng},
  number       = {6-7},
  pages        = {834--837},
  title        = {Green hydrophilic interaction chromatography using ethanol-water-carbon dioxide mixtures},
  url          = {http://dx.doi.org/10.1002/jssc.200900791},
  volume       = {33},
  year         = {2010},
}

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