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A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability

Shuang Wu, Wolf-Rüdiger Scheible, Dana Schindelasch, Hilde Van Den Daele UGent, Lieven De Veylder UGent and Tobias I Baskin (2010) DEVELOPMENT. 137(6). p.953-961
abstract
The caspase family protease, separase, is required at anaphase onset to cleave the cohesin complex, which joins sister chromatids. However, among eukaryotes, separases have acquired novel functions. Here, we show that Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant isolated previously on the basis of root swelling, harbors a mutation in At4g22970, the A. thaliana separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. Surprisingly, rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, which occur in other eukaryotes upon loss of separase activity. However, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1;1, excessive levels of which have been associated with altered microtubules and morphology. Cyclin B1;1 also accumulates in certain backgrounds in response to DNA damage, but we find no evidence for aberrant responses to DNA damage in rsw4. Our characterization of rsw4 leads us to hypothesize that plant separase, in addition to cleaving cohesin, regulates cyclin B1;1, with profound ramifications for morphogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DNA-DAMAGE, ANAPHASE, EXPRESSION, ROOT, Root morphology, radially swollen 4, Mitosis, Microtubules, DNA damage, Arabidopsis thaliana, MITOTIC EXIT, DROSOPHILA SEPARASE, TRANSCRIPTION FACTOR, CORTICAL MICROTUBULES, SISTER-CHROMATID COHESION, CELL-CYCLE
journal title
DEVELOPMENT
Development
volume
137
issue
6
pages
9 pages
Web of Science type
Article
Web of Science id
000275369100003
JCR category
DEVELOPMENTAL BIOLOGY
JCR impact factor
6.898 (2010)
JCR rank
4/38 (2010)
JCR quartile
1 (2010)
ISSN
0950-1991
DOI
10.1242/dev.041939
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
921071
handle
http://hdl.handle.net/1854/LU-921071
date created
2010-04-06 13:03:02
date last changed
2012-09-19 14:04:09
@article{921071,
  abstract     = {The caspase family protease, separase, is required at anaphase onset to cleave the cohesin complex, which joins sister chromatids. However, among eukaryotes, separases have acquired novel functions. Here, we show that Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant isolated previously on the basis of root swelling, harbors a mutation in At4g22970, the A. thaliana separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. Surprisingly, rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, which occur in other eukaryotes upon loss of separase activity. However, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1;1, excessive levels of which have been associated with altered microtubules and morphology. Cyclin B1;1 also accumulates in certain backgrounds in response to DNA damage, but we find no evidence for aberrant responses to DNA damage in rsw4. Our characterization of rsw4 leads us to hypothesize that plant separase, in addition to cleaving cohesin, regulates cyclin B1;1, with profound ramifications for morphogenesis.},
  author       = {Wu, Shuang and Scheible, Wolf-R{\"u}diger and Schindelasch, Dana and Van Den Daele, Hilde and De Veylder, Lieven and Baskin, Tobias I},
  issn         = {0950-1991},
  journal      = {DEVELOPMENT},
  keyword      = {DNA-DAMAGE,ANAPHASE,EXPRESSION,ROOT,Root morphology,radially swollen 4,Mitosis,Microtubules,DNA damage,Arabidopsis thaliana,MITOTIC EXIT,DROSOPHILA SEPARASE,TRANSCRIPTION FACTOR,CORTICAL MICROTUBULES,SISTER-CHROMATID COHESION,CELL-CYCLE},
  language     = {eng},
  number       = {6},
  pages        = {953--961},
  title        = {A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability},
  url          = {http://dx.doi.org/10.1242/dev.041939},
  volume       = {137},
  year         = {2010},
}

Chicago
Wu, Shuang, Wolf-Rüdiger Scheible, Dana Schindelasch, Hilde Van Den Daele, Lieven De Veylder, and Tobias I Baskin. 2010. “A Conditional Mutation in Arabidopsis Thaliana Separase Induces Chromosome Non-disjunction, Aberrant Morphogenesis and Cyclin B1;1 Stability.” Development 137 (6): 953–961.
APA
Wu, S., Scheible, W.-R., Schindelasch, D., Van Den Daele, H., De Veylder, L., & Baskin, T. I. (2010). A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability. DEVELOPMENT, 137(6), 953–961.
Vancouver
1.
Wu S, Scheible W-R, Schindelasch D, Van Den Daele H, De Veylder L, Baskin TI. A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability. DEVELOPMENT. 2010;137(6):953–61.
MLA
Wu, Shuang, Wolf-Rüdiger Scheible, Dana Schindelasch, et al. “A Conditional Mutation in Arabidopsis Thaliana Separase Induces Chromosome Non-disjunction, Aberrant Morphogenesis and Cyclin B1;1 Stability.” DEVELOPMENT 137.6 (2010): 953–961. Print.