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Optimization and validation of an analytical method to measure phenolic acids

Dries Knockaert (UGent) , Katleen Raes (UGent) , Christophe Wille (UGent) and John Van Camp (UGent)
(2009) COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES. 74(4). p.?-?
Author
Organization
Abstract
A method has been developed to separate and quantify phenolic acids (gallic-, caffeic-, p-coumaric-, o-coumaric and ferulic acid) and their microbial conversion products by means of high performance liquid chromatography (HPLC). The separation was performed using a Inertsil ODS-3, 3 µm (4.6 * 150 mm) column. Acetonitrile and 50 mM phosphoric acid formed the mobile phase. Quantification was done at three different wavelengths. The method has been validated by measuring the linearity, the limit of detection, the range, the limit of quantification and the reproducibility.

Citation

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MLA
Knockaert, Dries, et al. “Optimization and Validation of an Analytical Method to Measure Phenolic Acids.” COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES, vol. 74, no. 4, 2009, p. ?-?
APA
Knockaert, D., Raes, K., Wille, C., & Van Camp, J. (2009). Optimization and validation of an analytical method to measure phenolic acids. COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES, 74(4), ?-?
Chicago author-date
Knockaert, Dries, Katleen Raes, Christophe Wille, and John Van Camp. 2009. “Optimization and Validation of an Analytical Method to Measure Phenolic Acids.” In COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES, 74:?-?
Chicago author-date (all authors)
Knockaert, Dries, Katleen Raes, Christophe Wille, and John Van Camp. 2009. “Optimization and Validation of an Analytical Method to Measure Phenolic Acids.” In COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES, 74:?-?
Vancouver
1.
Knockaert D, Raes K, Wille C, Van Camp J. Optimization and validation of an analytical method to measure phenolic acids. In: COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES. 2009. p. ?-?
IEEE
[1]
D. Knockaert, K. Raes, C. Wille, and J. Van Camp, “Optimization and validation of an analytical method to measure phenolic acids,” in COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES, Leuven, Belgium, 2009, vol. 74, no. 4, p. ?-?
@inproceedings{915461,
  abstract     = {{A method has been developed to separate and quantify phenolic acids (gallic-, caffeic-, p-coumaric-, o-coumaric and ferulic acid) and their microbial conversion products by means of high performance liquid chromatography (HPLC).  The separation was performed using a Inertsil ODS-3, 3 µm  (4.6 * 150 mm) column. Acetonitrile and 50 mM phosphoric acid formed the mobile phase. Quantification was done at three different wavelengths. The method has been validated by measuring the linearity, the limit of detection, the range, the limit of quantification and the reproducibility.}},
  author       = {{Knockaert, Dries and Raes, Katleen and Wille, Christophe and Van Camp, John}},
  booktitle    = {{COMMUNICATIONS IN AGRICULTURAL AND APPLIED BIOLOGICAL SCIENCES}},
  issn         = {{1379-1176}},
  language     = {{eng}},
  location     = {{Leuven, Belgium}},
  number       = {{4}},
  pages        = {{?--?}},
  title        = {{Optimization and validation of an analytical method to measure phenolic acids}},
  volume       = {{74}},
  year         = {{2009}},
}