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Clonal expansion of the Belgian Phytophthora ramorum populations based on new microsatellite markers

Annelies Vercauteren UGent, Isabelle De Dobbelaere, NJ Grunwald, P Bonants, Erik Van Bockstaele UGent, Martine Maes and Kurt Heungens (2010) MOLECULAR ECOLOGY. 19(1). p.92-107
abstract
Co-existence of both mating types A1 and A2 within the EU1 lineage of Phytophthora ramorum has only been observed in Belgium, which begs the question whether sexual reproduction is occurring. A collection of 411 Belgian P. ramorum isolates was established during a 7-year survey. Our main objectives were genetic characterization of this population to test for sexual reproduction, determination of population structure, evolution and spread, and evaluation of the effectiveness and impact of control measures. Novel, polymorphic simple sequence repeat (SSR) markers were developed after screening 149 candidate loci. Eighty isolates of P. ramorum, broadly representing the Belgian population, were analyzed using four previously described and three newly identified polymorphic microsatellite loci as well as amplified fragment length polymorphisms. SSR analysis was most informative and was used to screen the entire Belgian population. Thirty multilocus genotypes were identified, but 68% of the isolates belonged to the main genotype EU1MG1. Although accumulated mutation events were detected, the overall level of genetic diversity within the Belgian isolates of P. ramorum appears to be limited, indicating a relatively recent clonal expansion. Based on our SSR analysis there is no evidence of sexual recombination in the Belgian population of P. ramorum. Metalaxyl use decreased the genetic diversity of P. ramorum until 2005, when the majority of the isolates had become resistant. Most genotypes were site-specific and despite systematic removal of symptomatic and neighbouring plants, some genotypes were detected over a period of several years at a single site, sometimes discontinuously, indicating (latent) survival of the pathogen at those sites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INFESTANS, PATHOGEN, MATING-TYPE, ORNAMENTAL PLANTS, PRACTICAL EXPERIENCE, METALAXYL RESISTANCE, 1ST REPORT, GENOTYPIC DIVERSITY, FUNGICIDE RESISTANCE, SUDDEN OAK DEATH, universal labelling, Sudden Oak Death, population genetics, oomycetes, metalaxyl resistance, nurseries
journal title
MOLECULAR ECOLOGY
Mol. Ecol.
volume
19
issue
1
pages
92 - 107
Web of Science type
Article
Web of Science id
000273005800011
JCR category
ECOLOGY
JCR impact factor
6.457 (2010)
JCR rank
5/129 (2010)
JCR quartile
1 (2010)
ISSN
0962-1083
DOI
10.1111/j.1365-294X.2009.04443.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
914880
handle
http://hdl.handle.net/1854/LU-914880
date created
2010-03-29 11:47:16
date last changed
2010-05-07 13:25:38
@article{914880,
  abstract     = {Co-existence of both mating types A1 and A2 within the EU1 lineage of Phytophthora ramorum has only been observed in Belgium, which begs the question whether sexual reproduction is occurring. A collection of 411 Belgian P. ramorum isolates was established during a 7-year survey. Our main objectives were genetic characterization of this population to test for sexual reproduction, determination of population structure, evolution and spread, and evaluation of the effectiveness and impact of control measures. Novel, polymorphic simple sequence repeat (SSR) markers were developed after screening 149 candidate loci. Eighty isolates of P. ramorum, broadly representing the Belgian population, were analyzed using four previously described and three newly identified polymorphic microsatellite loci as well as amplified fragment length polymorphisms. SSR analysis was most informative and was used to screen the entire Belgian population. Thirty multilocus genotypes were identified, but 68\% of the isolates belonged to the main genotype EU1MG1. Although accumulated mutation events were detected, the overall level of genetic diversity within the Belgian isolates of P. ramorum appears to be limited, indicating a relatively recent clonal expansion. Based on our SSR analysis there is no evidence of sexual recombination in the Belgian population of P. ramorum. Metalaxyl use decreased the genetic diversity of P. ramorum until 2005, when the majority of the isolates had become resistant. Most genotypes were site-specific and despite systematic removal of symptomatic and neighbouring plants, some genotypes were detected over a period of several years at a single site, sometimes discontinuously, indicating (latent) survival of the pathogen at those sites.},
  author       = {Vercauteren, Annelies and De Dobbelaere, Isabelle and Grunwald, NJ and Bonants, P and Van Bockstaele, Erik and Maes, Martine and Heungens, Kurt},
  issn         = {0962-1083},
  journal      = {MOLECULAR ECOLOGY},
  keyword      = {INFESTANS,PATHOGEN,MATING-TYPE,ORNAMENTAL PLANTS,PRACTICAL EXPERIENCE,METALAXYL RESISTANCE,1ST REPORT,GENOTYPIC DIVERSITY,FUNGICIDE RESISTANCE,SUDDEN OAK DEATH,universal labelling,Sudden Oak Death,population genetics,oomycetes,metalaxyl resistance,nurseries},
  language     = {eng},
  number       = {1},
  pages        = {92--107},
  title        = {Clonal expansion of the Belgian Phytophthora ramorum populations based on new microsatellite markers},
  url          = {http://dx.doi.org/10.1111/j.1365-294X.2009.04443.x},
  volume       = {19},
  year         = {2010},
}

Chicago
Vercauteren, Annelies, Isabelle De Dobbelaere, NJ Grunwald, P Bonants, Erik Van Bockstaele, Martine Maes, and Kurt Heungens. 2010. “Clonal Expansion of the Belgian Phytophthora Ramorum Populations Based on New Microsatellite Markers.” Molecular Ecology 19 (1): 92–107.
APA
Vercauteren, Annelies, De Dobbelaere, I., Grunwald, N., Bonants, P., Van Bockstaele, E., Maes, M., & Heungens, K. (2010). Clonal expansion of the Belgian Phytophthora ramorum populations based on new microsatellite markers. MOLECULAR ECOLOGY, 19(1), 92–107.
Vancouver
1.
Vercauteren A, De Dobbelaere I, Grunwald N, Bonants P, Van Bockstaele E, Maes M, et al. Clonal expansion of the Belgian Phytophthora ramorum populations based on new microsatellite markers. MOLECULAR ECOLOGY. 2010;19(1):92–107.
MLA
Vercauteren, Annelies, Isabelle De Dobbelaere, NJ Grunwald, et al. “Clonal Expansion of the Belgian Phytophthora Ramorum Populations Based on New Microsatellite Markers.” MOLECULAR ECOLOGY 19.1 (2010): 92–107. Print.