Ghent University Academic Bibliography

Advanced

Probing plant membranes with FM dyes: tracking, dragging or blocking?

Adriana Jelinkova, Katerina Malinska, Sibu Simon, Jürgen Kleine-Vehn UGent, Marketa Parezova, Premysl Pejchar, Martin Kubes, Jan Martinec, Jiri Friml UGent and Eva Zazimalova, et al. (2010) PLANT JOURNAL. 61(5). p.883-892
abstract
Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity (lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using simultaneous labelling with FM dyes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VESICLE TRAFFICKING, PERMEANT BLOCKER, PIN PROTEINS, ARABIDOPSIS, ENDOCYTOSIS, endocytosis, tobacco BY-2 cells, plasma membrane, FM 1-43, FM 4-64, PLASMA-MEMBRANE, CULTURED TOBACCO CELLS, AUXIN EFFLUX, VISUALIZATION, EXOCYTOSIS
journal title
PLANT JOURNAL
Plant J.
volume
61
issue
5
pages
10 pages
Web of Science type
Article
Web of Science id
000274805600014
JCR category
PLANT SCIENCES
JCR impact factor
6.948 (2010)
JCR rank
6/185 (2010)
JCR quartile
1 (2010)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2009.04102.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
912477
handle
http://hdl.handle.net/1854/LU-912477
date created
2010-03-24 17:30:12
date last changed
2012-09-19 14:03:49
@article{912477,
  abstract     = {Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity (lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using simultaneous labelling with FM dyes.},
  author       = {Jelinkova, Adriana and Malinska, Katerina and Simon, Sibu and Kleine-Vehn, J{\"u}rgen and Parezova, Marketa and Pejchar, Premysl and Kubes, Martin and Martinec, Jan and Friml, Jiri and Zazimalova, Eva and Petrasek, Jan},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {VESICLE TRAFFICKING,PERMEANT BLOCKER,PIN PROTEINS,ARABIDOPSIS,ENDOCYTOSIS,endocytosis,tobacco BY-2 cells,plasma membrane,FM 1-43,FM 4-64,PLASMA-MEMBRANE,CULTURED TOBACCO CELLS,AUXIN EFFLUX,VISUALIZATION,EXOCYTOSIS},
  language     = {eng},
  number       = {5},
  pages        = {883--892},
  title        = {Probing plant membranes with FM dyes: tracking, dragging or blocking?},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2009.04102.x},
  volume       = {61},
  year         = {2010},
}

Chicago
Jelinkova, Adriana, Katerina Malinska, Sibu Simon, Jürgen Kleine-Vehn, Marketa Parezova, Premysl Pejchar, Martin Kubes, et al. 2010. “Probing Plant Membranes with FM Dyes: Tracking, Dragging or Blocking?” Plant Journal 61 (5): 883–892.
APA
Jelinkova, A., Malinska, K., Simon, S., Kleine-Vehn, J., Parezova, M., Pejchar, P., Kubes, M., et al. (2010). Probing plant membranes with FM dyes: tracking, dragging or blocking? PLANT JOURNAL, 61(5), 883–892.
Vancouver
1.
Jelinkova A, Malinska K, Simon S, Kleine-Vehn J, Parezova M, Pejchar P, et al. Probing plant membranes with FM dyes: tracking, dragging or blocking? PLANT JOURNAL. 2010;61(5):883–92.
MLA
Jelinkova, Adriana, Katerina Malinska, Sibu Simon, et al. “Probing Plant Membranes with FM Dyes: Tracking, Dragging or Blocking?” PLANT JOURNAL 61.5 (2010): 883–892. Print.