Advanced search
1 file | 769.81 KB

Probing plant membranes with FM dyes: tracking, dragging or blocking?

(2010) PLANT JOURNAL. 61(5). p.883-892
Author
Organization
Abstract
Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity (lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using simultaneous labelling with FM dyes.
Keywords
VESICLE TRAFFICKING, PERMEANT BLOCKER, PIN PROTEINS, ARABIDOPSIS, ENDOCYTOSIS, endocytosis, tobacco BY-2 cells, plasma membrane, FM 1-43, FM 4-64, PLASMA-MEMBRANE, CULTURED TOBACCO CELLS, AUXIN EFFLUX, VISUALIZATION, EXOCYTOSIS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 769.81 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Jelinkova, Adriana, Katerina Malinska, Sibu Simon, Jürgen Kleine-Vehn, Marketa Parezova, Premysl Pejchar, Martin Kubes, et al. 2010. “Probing Plant Membranes with FM Dyes: Tracking, Dragging or Blocking?” Plant Journal 61 (5): 883–892.
APA
Jelinkova, A., Malinska, K., Simon, S., Kleine-Vehn, J., Parezova, M., Pejchar, P., Kubes, M., et al. (2010). Probing plant membranes with FM dyes: tracking, dragging or blocking? PLANT JOURNAL, 61(5), 883–892.
Vancouver
1.
Jelinkova A, Malinska K, Simon S, Kleine-Vehn J, Parezova M, Pejchar P, et al. Probing plant membranes with FM dyes: tracking, dragging or blocking? PLANT JOURNAL. 2010;61(5):883–92.
MLA
Jelinkova, Adriana, Katerina Malinska, Sibu Simon, et al. “Probing Plant Membranes with FM Dyes: Tracking, Dragging or Blocking?” PLANT JOURNAL 61.5 (2010): 883–892. Print.
@article{912477,
  abstract     = {Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity (lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using simultaneous labelling with FM dyes.},
  author       = {Jelinkova, Adriana and Malinska, Katerina and Simon, Sibu and Kleine-Vehn, J{\"u}rgen and Parezova, Marketa and Pejchar, Premysl and Kubes, Martin and Martinec, Jan and Friml, Jiri and Zazimalova, Eva and Petrasek, Jan},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {VESICLE TRAFFICKING,PERMEANT BLOCKER,PIN PROTEINS,ARABIDOPSIS,ENDOCYTOSIS,endocytosis,tobacco BY-2 cells,plasma membrane,FM 1-43,FM 4-64,PLASMA-MEMBRANE,CULTURED TOBACCO CELLS,AUXIN EFFLUX,VISUALIZATION,EXOCYTOSIS},
  language     = {eng},
  number       = {5},
  pages        = {883--892},
  title        = {Probing plant membranes with FM dyes: tracking, dragging or blocking?},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2009.04102.x},
  volume       = {61},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: