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A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution

Ora Hazak, Daria Bloch, Limor Poraty, Hasana Sternberg, Jing Zhang UGent, Jiri Friml UGent and Shaul Yalovsky (2010) PLOS BIOLOGY. 8(1).
abstract
Development in multicellular organisms depends on the ability of individual cells to coordinate their behavior by means of small signaling molecules to form correctly patterned tissues. In plants, a unique mechanism of directional transport of the signaling molecule auxin between cells connects cell polarity and tissue patterning and thus is required for many aspects of plant development. Direction of auxin flow is determined by polar subcellular localization of PIN auxin efflux transporters. Dynamic PIN polar localization results from the constitutive endocytic cycling to and from the plasma membrane, but it is not well understood how this mechanism connects to regulators of cell polarity. The Rho family small GTPases ROPs/RACs are master regulators of cell polarity, however their role in regulating polar protein trafficking and polar auxin transport has not been established. Here, by analysis of mutants and transgenic plants, we show that the ROP interactor and polarity regulator scaffold protein ICR1 is required for recruitment of PIN proteins to the polar domains at the plasma membrane. icr1 mutant embryos and plants display an a array of severe developmental aberrations that are caused by compromised differential auxin distribution. ICR1 functions at the plasma membrane where it is required for exocytosis but does not recycle together with PINs. ICR1 expression is quickly induced by auxin but is suppressed at the positions of stable auxin maxima in the hypophysis and later in the embryonic and mature root meristems. Our results imply that ICR1 is part of an auxin regulated positive feedback loop realized by a unique integration of auxin-dependent transcriptional regulation into ROP-mediated modulation of cell polarity. Thus, ICR1 forms an auxin-modulated link between cell polarity, exocytosis, and auxin transport-dependent tissue patterning.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
POLAR GROWTH, ACTIN DYNAMICS, STEM-CELL NICHE, POLLEN-TUBE GROWTH, PLASMA-MEMBRANE, ARABIDOPSIS-THALIANA, PLANT DEVELOPMENT, ROOT, VESICLE TRAFFICKING, TIP GROWTH
journal title
PLOS BIOLOGY
PLoS. Biol.
volume
8
issue
1
pages
16 pages
Web of Science type
Article
Web of Science id
000275256800014
JCR category
BIOLOGY
JCR impact factor
12.469 (2010)
JCR rank
1/84 (2010)
JCR quartile
1 (2010)
ISSN
1544-9173
DOI
10.1371/journal.pbio.1000282
language
English
UGent publication?
yes
classification
A1
additional info
article no. e1000282 (16 p.)
copyright statement
I have retained and own the full copyright for this publication
id
912465
handle
http://hdl.handle.net/1854/LU-912465
date created
2010-03-24 17:29:59
date last changed
2012-09-19 14:03:48
@article{912465,
  abstract     = {Development in multicellular organisms depends on the ability of individual cells to coordinate their behavior by means of small signaling molecules to form correctly patterned tissues. In plants, a unique mechanism of directional transport of the signaling molecule auxin between cells connects cell polarity and tissue patterning and thus is required for many aspects of plant development. Direction of auxin flow is determined by polar subcellular localization of PIN auxin efflux transporters. Dynamic PIN polar localization results from the constitutive endocytic cycling to and from the plasma membrane, but it is not well understood how this mechanism connects to regulators of cell polarity. The Rho family small GTPases ROPs/RACs are master regulators of cell polarity, however their role in regulating polar protein trafficking and polar auxin transport has not been established. Here, by analysis of mutants and transgenic plants, we show that the ROP interactor and polarity regulator scaffold protein ICR1 is required for recruitment of PIN proteins to the polar domains at the plasma membrane. icr1 mutant embryos and plants display an a array of severe developmental aberrations that are caused by compromised differential auxin distribution. ICR1 functions at the plasma membrane where it is required for exocytosis but does not recycle together with PINs. ICR1 expression is quickly induced by auxin but is suppressed at the positions of stable auxin maxima in the hypophysis and later in the embryonic and mature root meristems. Our results imply that ICR1 is part of an auxin regulated positive feedback loop realized by a unique integration of auxin-dependent transcriptional regulation into ROP-mediated modulation of cell polarity. Thus, ICR1 forms an auxin-modulated link between cell polarity, exocytosis, and auxin transport-dependent tissue patterning.},
  author       = {Hazak, Ora and Bloch, Daria and Poraty, Limor and Sternberg, Hasana and Zhang, Jing and Friml, Jiri and Yalovsky, Shaul},
  issn         = {1544-9173},
  journal      = {PLOS BIOLOGY},
  keyword      = {POLAR GROWTH,ACTIN DYNAMICS,STEM-CELL NICHE,POLLEN-TUBE GROWTH,PLASMA-MEMBRANE,ARABIDOPSIS-THALIANA,PLANT DEVELOPMENT,ROOT,VESICLE TRAFFICKING,TIP GROWTH},
  language     = {eng},
  number       = {1},
  pages        = {16},
  title        = {A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution},
  url          = {http://dx.doi.org/10.1371/journal.pbio.1000282},
  volume       = {8},
  year         = {2010},
}

Chicago
Hazak, Ora, Daria Bloch, Limor Poraty, Hasana Sternberg, Jing Zhang, Jiri Friml, and Shaul Yalovsky. 2010. “A Rho Scaffold Integrates the Secretory System with Feedback Mechanisms in Regulation of Auxin Distribution.” Plos Biology 8 (1).
APA
Hazak, O., Bloch, D., Poraty, L., Sternberg, H., Zhang, J., Friml, J., & Yalovsky, S. (2010). A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution. PLOS BIOLOGY, 8(1).
Vancouver
1.
Hazak O, Bloch D, Poraty L, Sternberg H, Zhang J, Friml J, et al. A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution. PLOS BIOLOGY. 2010;8(1).
MLA
Hazak, Ora, Daria Bloch, Limor Poraty, et al. “A Rho Scaffold Integrates the Secretory System with Feedback Mechanisms in Regulation of Auxin Distribution.” PLOS BIOLOGY 8.1 (2010): n. pag. Print.