Ghent University Academic Bibliography

Advanced

Combination of liquid-chromatography tandem mass spectrometry in different scan modes with human and chimeric mouse urine for the study of steroid metabolism

Oscar Juan Pozo Mendoza UGent, Leen Lootens UGent, Peter Van Eenoo UGent, Koen Deventer UGent, Philip Meuleman UGent, Geert Leroux-Roels UGent, MK Parr, W Schanzer and Frans Delbeke UGent (2009) DRUG TESTING AND ANALYSIS. 1(11-12). p.554-567
abstract
Anabolic steroids are among the most frequently detected compounds in doping analysis. They are extensively metabolized and therefore an in-depth knowledge about steroid metabolism is needed. In this study, a liquid chromatography tandem mass spectometry (LC-MS/MS) method based on a precursor ion scan with a uPA-SCID mouse with humanized liver (a chimeric mouse) was explored for the detection of steroid metabolism. Methandienone was used as a model compound. The application of the precursor ion scan method in positive human samples and chimeric mice samples after methandienone administration allowed the detection of most steroid metabolites without any structural restriction. Three hitherto unreported metabolites were found using this approach. These metabolites were characterized using LC-MS/MS and feasible structures were proposed. The structure of one of them, 6-ene-epimethandienone, was confirmed by the synthesis of the reference compound. A selected reaction monitoring (SRM) method for the specific detection of all these metabolites has been developed. The application of this method to several human and chimeric mouse samples confirmed that more than 80% of the steroid metabolites were found in both samples. Only metabolites that are poorly detectable by LC-MS/MS were not detected in some urine samples. The metabolic nature of the unreported metabolites was also confirmed. A global strategy for the detection of steroid metabolites combining both human and chimeric mouse urine is proposed. Copyright (C) 2009 John Wiley & Sons, Ltd.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BIOSYNTHESIS, IN-VITRO, ANABOLIC-STEROIDS, STRUCTURE ELUCIDATION, SCREENING METHOD, IDENTIFICATION, METANDIENONE, DISCOVERY, METHANDIENONE, TETRAHYDROGESTRINONE
journal title
DRUG TESTING AND ANALYSIS
Drug Test. Anal.
volume
1
issue
11-12
pages
14 pages
publisher
JOHN WILEY & SONS LTD
place of publication
CHICHESTER
Web of Science type
Article
Web of Science id
000275021000013
ISSN
1942-7603
DOI
10.1002/dta.56
language
English
UGent publication?
yes
classification
A1
id
910772
handle
http://hdl.handle.net/1854/LU-910772
date created
2010-03-23 11:42:54
date last changed
2010-03-31 15:37:21
@article{910772,
  abstract     = {Anabolic steroids are among the most frequently detected compounds in doping analysis. They are extensively metabolized and therefore an in-depth knowledge about steroid metabolism is needed. In this study, a liquid chromatography tandem mass spectometry (LC-MS/MS) method based on a precursor ion scan with a uPA-SCID mouse with humanized liver (a chimeric mouse) was explored for the detection of steroid metabolism. Methandienone was used as a model compound. The application of the precursor ion scan method in positive human samples and chimeric mice samples after methandienone administration allowed the detection of most steroid metabolites without any structural restriction. Three hitherto unreported metabolites were found using this approach. These metabolites were characterized using LC-MS/MS and feasible structures were proposed. The structure of one of them, 6-ene-epimethandienone, was confirmed by the synthesis of the reference compound. A selected reaction monitoring (SRM) method for the specific detection of all these metabolites has been developed. The application of this method to several human and chimeric mouse samples confirmed that more than 80\% of the steroid metabolites were found in both samples. Only metabolites that are poorly detectable by LC-MS/MS were not detected in some urine samples. The metabolic nature of the unreported metabolites was also confirmed. A global strategy for the detection of steroid metabolites combining both human and chimeric mouse urine is proposed. Copyright (C) 2009 John Wiley \& Sons, Ltd.},
  author       = {Pozo Mendoza, Oscar Juan and Lootens, Leen and Van Eenoo, Peter and Deventer, Koen and Meuleman, Philip and Leroux-Roels, Geert and Parr, MK and Schanzer, W and Delbeke, Frans},
  issn         = {1942-7603},
  journal      = {DRUG TESTING AND ANALYSIS},
  keyword      = {BIOSYNTHESIS,IN-VITRO,ANABOLIC-STEROIDS,STRUCTURE ELUCIDATION,SCREENING METHOD,IDENTIFICATION,METANDIENONE,DISCOVERY,METHANDIENONE,TETRAHYDROGESTRINONE},
  language     = {eng},
  number       = {11-12},
  pages        = {554--567},
  publisher    = {JOHN WILEY \& SONS LTD},
  title        = {Combination of liquid-chromatography tandem mass spectrometry in different scan modes with human and chimeric mouse urine for the study of steroid metabolism},
  url          = {http://dx.doi.org/10.1002/dta.56},
  volume       = {1},
  year         = {2009},
}

Chicago
Pozo Mendoza, Oscar Juan, Leen Lootens, Peter Van Eenoo, Koen Deventer, Philip Meuleman, Geert Leroux-Roels, MK Parr, W Schanzer, and Frans Delbeke. 2009. “Combination of Liquid-chromatography Tandem Mass Spectrometry in Different Scan Modes with Human and Chimeric Mouse Urine for the Study of Steroid Metabolism.” Drug Testing and Analysis 1 (11-12): 554–567.
APA
Pozo Mendoza, O. J., Lootens, L., Van Eenoo, P., Deventer, K., Meuleman, P., Leroux-Roels, G., Parr, M., et al. (2009). Combination of liquid-chromatography tandem mass spectrometry in different scan modes with human and chimeric mouse urine for the study of steroid metabolism. DRUG TESTING AND ANALYSIS, 1(11-12), 554–567.
Vancouver
1.
Pozo Mendoza OJ, Lootens L, Van Eenoo P, Deventer K, Meuleman P, Leroux-Roels G, et al. Combination of liquid-chromatography tandem mass spectrometry in different scan modes with human and chimeric mouse urine for the study of steroid metabolism. DRUG TESTING AND ANALYSIS. CHICHESTER: JOHN WILEY & SONS LTD; 2009;1(11-12):554–67.
MLA
Pozo Mendoza, Oscar Juan, Leen Lootens, Peter Van Eenoo, et al. “Combination of Liquid-chromatography Tandem Mass Spectrometry in Different Scan Modes with Human and Chimeric Mouse Urine for the Study of Steroid Metabolism.” DRUG TESTING AND ANALYSIS 1.11-12 (2009): 554–567. Print.