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Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA

Ilse Becue UGent, Christof Van Poucke UGent, Jeroen CW Rijk, Toine FH Bovee, Michel Nielen and Carlos Van Peteghem UGent (2010) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 396(2). p.799-808
abstract
DHEA (3 beta-hydroxy-androst-5-en-17-one) is a natural steroid prohormone. Despite a lack of information on the effect, DHEA and other prohormones are frequently used as a food supplement by body-builders. DHEA is suspected for growth promoting abuse in cattle as well. Considering the latter, urine samples from a previous exposure study in which calves were exposed to 1 g DHEA per day for 7 days, were used. The calves were divided in three groups: one orally treated, one intramuscularly injected, and a control group. The effect of this treatment on the urinary profile of several precursors and metabolites of DHEA was investigated. Urine samples were collected several days before and during the 7 days of administration and were submitted to a clean-up procedure consisting of a separation of the different conjugates (free, glucuronidated, and sulfated forms) of each compound on a SAX column (Varian). An LC-MS/MS method was developed for the detection and quantification of several metabolites of the pathway of DHEA including 17 alpha- and 17 beta-testosterone, 4-androstenedione, 5-androstenediol, pregnenolone, and hydroxypregnenolone. Elevated levels of DHEA, 5-androstenediol, and 17 alpha-testosterone were observed in the free and sulfated fraction of the urine of the treated calves, thus indicating that the administered DHEA is metabolized mainly by the a dagger(5)-pathway with 5-androstenediol as the intermediate. Sulfoconjugates of DHEA and its metabolites were found to constitute the largest proportion of the urinary metabolites. The free form was also present, but in a lesser extent than the sulfated form, while glucuronides were negligible.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (proceedingsPaper)
publication status
published
subject
keyword
SUPPLEMENTS, BIOASSAY, MASS-SPECTROMETRY, ANDROGEN PROFILE, Prohormones, 5-androstenediol, Urine, Metabolites, DHEA, LC-MS/MS, MEN, DEHYDROEPIANDROSTERONE, GAS, PROHORMONES, CONSEQUENCES
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
396
issue
2
pages
10 pages
conference name
15th International Symposium on Pollutant Responses in Marine Organisms (PRIMO15)
conference location
Bordeaux, France
conference start
2009-05-17
conference end
2009-05-20
Web of Science type
Proceedings Paper
Web of Science id
000272910400028
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.841 (2010)
JCR rank
9/71 (2010)
JCR quartile
1 (2010)
ISSN
1618-2642
DOI
10.1007/s00216-009-3265-z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
906373
handle
http://hdl.handle.net/1854/LU-906373
date created
2010-03-16 16:12:27
date last changed
2010-04-08 14:02:36
@article{906373,
  abstract     = {DHEA (3 beta-hydroxy-androst-5-en-17-one) is a natural steroid prohormone. Despite a lack of information on the effect, DHEA and other prohormones are frequently used as a food supplement by body-builders. DHEA is suspected for growth promoting abuse in cattle as well. Considering the latter, urine samples from a previous exposure study in which calves were exposed to 1 g DHEA per day for 7 days, were used. The calves were divided in three groups: one orally treated, one intramuscularly injected, and a control group. The effect of this treatment on the urinary profile of several precursors and metabolites of DHEA was investigated. Urine samples were collected several days before and during the 7 days of administration and were submitted to a clean-up procedure consisting of a separation of the different conjugates (free, glucuronidated, and sulfated forms) of each compound on a SAX column (Varian). An LC-MS/MS method was developed for the detection and quantification of several metabolites of the pathway of DHEA including 17 alpha- and 17 beta-testosterone, 4-androstenedione, 5-androstenediol, pregnenolone, and hydroxypregnenolone. Elevated levels of DHEA, 5-androstenediol, and 17 alpha-testosterone were observed in the free and sulfated fraction of the urine of the treated calves, thus indicating that the administered DHEA is metabolized mainly by the a dagger(5)-pathway with 5-androstenediol as the intermediate. Sulfoconjugates of DHEA and its metabolites were found to constitute the largest proportion of the urinary metabolites. The free form was also present, but in a lesser extent than the sulfated form, while glucuronides were negligible.},
  author       = {Becue, Ilse and Van Poucke, Christof and Rijk, Jeroen CW and Bovee, Toine FH and Nielen, Michel and Van Peteghem, Carlos},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {SUPPLEMENTS,BIOASSAY,MASS-SPECTROMETRY,ANDROGEN PROFILE,Prohormones,5-androstenediol,Urine,Metabolites,DHEA,LC-MS/MS,MEN,DEHYDROEPIANDROSTERONE,GAS,PROHORMONES,CONSEQUENCES},
  language     = {eng},
  location     = {Bordeaux, France},
  number       = {2},
  pages        = {799--808},
  title        = {Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA},
  url          = {http://dx.doi.org/10.1007/s00216-009-3265-z},
  volume       = {396},
  year         = {2010},
}

Chicago
Becue, Ilse, Christof Van Poucke, Jeroen CW Rijk, Toine FH Bovee, Michel Nielen, and Carlos Van Peteghem. 2010. “Investigation of Urinary Steroid Metabolites in Calf Urine After Oral and Intramuscular Administration of DHEA.” Analytical and Bioanalytical Chemistry 396 (2): 799–808.
APA
Becue, I., Van Poucke, C., Rijk, J. C., Bovee, T. F., Nielen, M., & Van Peteghem, C. (2010). Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 396(2), 799–808. Presented at the 15th International Symposium on Pollutant Responses in Marine Organisms (PRIMO15).
Vancouver
1.
Becue I, Van Poucke C, Rijk JC, Bovee TF, Nielen M, Van Peteghem C. Investigation of urinary steroid metabolites in calf urine after oral and intramuscular administration of DHEA. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2010;396(2):799–808.
MLA
Becue, Ilse, Christof Van Poucke, Jeroen CW Rijk, et al. “Investigation of Urinary Steroid Metabolites in Calf Urine After Oral and Intramuscular Administration of DHEA.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 396.2 (2010): 799–808. Print.