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Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study

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Abstract
The aims of the study were to study the effect of pre-operative treatment on the expression of tumour-related proteins and to correlate the expression of these proteins with response and survival of patients with advanced rectal cancer. Tissue micro-arrays from pre- and post-treatment biopsies of 99 patients with rectal cancer treated with pre-operative (chemo)radiotherapy were stained for epidermal growth factor receptor (EGFR), carbonic anhydrase IX, Ki67, vascular endothelial growth factor, cyclo-oxygenase 2 (COX-2) and cleaved cytokeratin 18 (c-CK18). Also, fibro-inflammatory alterations after treatment were evaluated. Pre-operative (chemo)radiotherapy caused fibro-inflammatory changes, a downregulation of proliferation (Ki67) and EGFR and an upregulation of apoptosis (cleaved CK18). Patients with a good regression during pre-operative treatment showed less proliferating and apoptotic cells in the resection specimen. Multivariate analysis showed that T downstaging, fibro-inflammatory changes in the resection specimen and COX-2 expression in the biopsy correlated with overall survival. Pre-operative treatment has an effect on proliferation, apoptosis, inflammation and EGFR expression. The classical clinical parameters as well as fibro-inflammatory changes and COX-2 expression seem most valuable as predictors for survival.
Keywords
TOTAL MESORECTAL EXCISION, EPIDERMAL-GROWTH-FACTOR, CIRCUMFERENTIAL MARGIN INVOLVEMENT, COLORECTAL-CANCER, CELL-PROLIFERATION, CYCLOOXYGENASE-2 EXPRESSION, RADIATION-THERAPY, PREOPERATIVE RADIOCHEMOTHERAPY, ADJUVANT THERAPY, FACTOR RECEPTOR

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Chicago
Debucquoy, Annelies, Laurence Goethals, Louis Libbrecht, Christiaan Perneel, Karel Geboes, Nadine Ectors, William McBride, and Karin Haustermans. 2009. “Molecular and Clinico-pathological Markers in Rectal Cancer: a Tissue Micro-array Study.” International Journal of Colorectal Disease 24 (2): 129–138.
APA
Debucquoy, Annelies, Goethals, L., Libbrecht, L., Perneel, C., Geboes, K., Ectors, N., McBride, W., et al. (2009). Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE, 24(2), 129–138.
Vancouver
1.
Debucquoy A, Goethals L, Libbrecht L, Perneel C, Geboes K, Ectors N, et al. Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study. INTERNATIONAL JOURNAL OF COLORECTAL DISEASE. NEW YORK: Springer; 2009;24(2):129–38.
MLA
Debucquoy, Annelies, Laurence Goethals, Louis Libbrecht, et al. “Molecular and Clinico-pathological Markers in Rectal Cancer: a Tissue Micro-array Study.” INTERNATIONAL JOURNAL OF COLORECTAL DISEASE 24.2 (2009): 129–138. Print.
@article{903920,
  abstract     = {The aims of the study were to study the effect of pre-operative treatment on the expression of tumour-related proteins and to correlate the expression of these proteins with response and survival of patients with advanced rectal cancer.

Tissue micro-arrays from pre- and post-treatment biopsies of 99 patients with rectal cancer treated with pre-operative (chemo)radiotherapy were stained for epidermal growth factor receptor (EGFR), carbonic anhydrase IX, Ki67, vascular endothelial growth factor, cyclo-oxygenase 2 (COX-2) and cleaved cytokeratin 18 (c-CK18). Also, fibro-inflammatory alterations after treatment were evaluated.

Pre-operative (chemo)radiotherapy caused fibro-inflammatory changes, a downregulation of proliferation (Ki67) and EGFR and an upregulation of apoptosis (cleaved CK18). Patients with a good regression during pre-operative treatment showed less proliferating and apoptotic cells in the resection specimen. Multivariate analysis showed that T downstaging, fibro-inflammatory changes in the resection specimen and COX-2 expression in the biopsy correlated with overall survival.

Pre-operative treatment has an effect on proliferation, apoptosis, inflammation and EGFR expression. The classical clinical parameters as well as fibro-inflammatory changes and COX-2 expression seem most valuable as predictors for survival.},
  author       = {Debucquoy, Annelies and Goethals, Laurence and Libbrecht, Louis and Perneel, Christiaan and Geboes, Karel and Ectors, Nadine and McBride, William and Haustermans, Karin},
  issn         = {0179-1958},
  journal      = {INTERNATIONAL JOURNAL OF COLORECTAL DISEASE},
  keyword      = {TOTAL MESORECTAL EXCISION,EPIDERMAL-GROWTH-FACTOR,CIRCUMFERENTIAL MARGIN INVOLVEMENT,COLORECTAL-CANCER,CELL-PROLIFERATION,CYCLOOXYGENASE-2 EXPRESSION,RADIATION-THERAPY,PREOPERATIVE RADIOCHEMOTHERAPY,ADJUVANT THERAPY,FACTOR RECEPTOR},
  language     = {eng},
  number       = {2},
  pages        = {129--138},
  publisher    = {Springer},
  title        = {Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study},
  url          = {http://dx.doi.org/10.1007/s00384-008-0608-8},
  volume       = {24},
  year         = {2009},
}

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