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Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance

L Lin, Louis Libbrecht (UGent) , J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst and J Fevery
(2009) JOURNAL OF MEDICAL VIROLOGY. 81(9). p.1569-1575
Author
Organization
Abstract
HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand-specific HCV real-time RT-PCR assays were developed and applied to livers explanted because of end-stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)- and negative (NS)strand RNA did correlate with each other, with PS/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV-PS or -NS RNA did not correlate with serum HCV-RNA levels or with genotypes. A high HCV envelope-2 protein expression correlated with a low NS concentration. HCV-PS and -NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS/PS, a marker of viral replication, correlated with the severity of the recurrent post-transplant hepatitis caused by HCV. This suggests the existence of an extra-hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver.
Keywords
TRANSPLANTATION, INFECTION, PROTEIN, BLOOD MONONUCLEAR-CELLS, HEPATITIS-C-VIRUS, ANTIVIRAL THERAPY, OXIDATIVE STRESS, MINUS-STRAND, ACTIVATION, RNA

Citation

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MLA
Lin, L., et al. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” JOURNAL OF MEDICAL VIROLOGY, vol. 81, no. 9, WILEY-LISS, 2009, pp. 1569–75, doi:10.1002/jmv.21510.
APA
Lin, L., Libbrecht, L., Verbeeck, J., Verslype, C., Roskams, T., van Pelt, J., … Fevery, J. (2009). Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance. JOURNAL OF MEDICAL VIROLOGY, 81(9), 1569–1575. https://doi.org/10.1002/jmv.21510
Chicago author-date
Lin, L, Louis Libbrecht, J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst, and J Fevery. 2009. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” JOURNAL OF MEDICAL VIROLOGY 81 (9): 1569–75. https://doi.org/10.1002/jmv.21510.
Chicago author-date (all authors)
Lin, L, Louis Libbrecht, J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst, and J Fevery. 2009. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” JOURNAL OF MEDICAL VIROLOGY 81 (9): 1569–1575. doi:10.1002/jmv.21510.
Vancouver
1.
Lin L, Libbrecht L, Verbeeck J, Verslype C, Roskams T, van Pelt J, et al. Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance. JOURNAL OF MEDICAL VIROLOGY. 2009;81(9):1569–75.
IEEE
[1]
L. Lin et al., “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance,” JOURNAL OF MEDICAL VIROLOGY, vol. 81, no. 9, pp. 1569–1575, 2009.
@article{903828,
  abstract     = {{HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand-specific HCV real-time RT-PCR assays were developed and applied to livers explanted because of end-stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)- and negative (NS)strand RNA did correlate with each other, with PS/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV-PS or -NS RNA did not correlate with serum HCV-RNA levels or with genotypes. A high HCV envelope-2 protein expression correlated with a low NS concentration. HCV-PS and -NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS/PS, a marker of viral replication, correlated with the severity of the recurrent post-transplant hepatitis caused by HCV. This suggests the existence of an extra-hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver.}},
  author       = {{Lin, L and Libbrecht, Louis and Verbeeck, J and Verslype, C and Roskams, T and van Pelt, J and Van Ranst, M and Fevery, J}},
  issn         = {{0146-6615}},
  journal      = {{JOURNAL OF MEDICAL VIROLOGY}},
  keywords     = {{TRANSPLANTATION,INFECTION,PROTEIN,BLOOD MONONUCLEAR-CELLS,HEPATITIS-C-VIRUS,ANTIVIRAL THERAPY,OXIDATIVE STRESS,MINUS-STRAND,ACTIVATION,RNA}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1569--1575}},
  publisher    = {{WILEY-LISS}},
  title        = {{Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance}},
  url          = {{http://dx.doi.org/10.1002/jmv.21510}},
  volume       = {{81}},
  year         = {{2009}},
}
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