Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance
- Author
- L Lin, Louis Libbrecht (UGent) , J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst and J Fevery
- Organization
- Abstract
- HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand-specific HCV real-time RT-PCR assays were developed and applied to livers explanted because of end-stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)- and negative (NS)strand RNA did correlate with each other, with PS/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV-PS or -NS RNA did not correlate with serum HCV-RNA levels or with genotypes. A high HCV envelope-2 protein expression correlated with a low NS concentration. HCV-PS and -NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS/PS, a marker of viral replication, correlated with the severity of the recurrent post-transplant hepatitis caused by HCV. This suggests the existence of an extra-hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver.
- Keywords
- TRANSPLANTATION, INFECTION, PROTEIN, BLOOD MONONUCLEAR-CELLS, HEPATITIS-C-VIRUS, ANTIVIRAL THERAPY, OXIDATIVE STRESS, MINUS-STRAND, ACTIVATION, RNA
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-903828
- MLA
- Lin, L et al. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” JOURNAL OF MEDICAL VIROLOGY 81.9 (2009): 1569–1575. Print.
- APA
- Lin, L., Libbrecht, L., Verbeeck, J., Verslype, C., Roskams, T., van Pelt, J., Van Ranst, M., et al. (2009). Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance. JOURNAL OF MEDICAL VIROLOGY, 81(9), 1569–1575.
- Chicago author-date
- Lin, L, Louis Libbrecht, J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst, and J Fevery. 2009. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” Journal of Medical Virology 81 (9): 1569–1575.
- Chicago author-date (all authors)
- Lin, L, Louis Libbrecht, J Verbeeck, C Verslype, T Roskams, J van Pelt, M Van Ranst, and J Fevery. 2009. “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance.” Journal of Medical Virology 81 (9): 1569–1575.
- Vancouver
- 1.Lin L, Libbrecht L, Verbeeck J, Verslype C, Roskams T, van Pelt J, et al. Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance. JOURNAL OF MEDICAL VIROLOGY. HOBOKEN: WILEY-LISS; 2009;81(9):1569–75.
- IEEE
- [1]L. Lin et al., “Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance,” JOURNAL OF MEDICAL VIROLOGY, vol. 81, no. 9, pp. 1569–1575, 2009.
@article{903828,
abstract = {{HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand-specific HCV real-time RT-PCR assays were developed and applied to livers explanted because of end-stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)- and negative (NS)strand RNA did correlate with each other, with PS/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV-PS or -NS RNA did not correlate with serum HCV-RNA levels or with genotypes. A high HCV envelope-2 protein expression correlated with a low NS concentration. HCV-PS and -NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS/PS, a marker of viral replication, correlated with the severity of the recurrent post-transplant hepatitis caused by HCV. This suggests the existence of an extra-hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver.}},
author = {{Lin, L and Libbrecht, Louis and Verbeeck, J and Verslype, C and Roskams, T and van Pelt, J and Van Ranst, M and Fevery, J}},
issn = {{0146-6615}},
journal = {{JOURNAL OF MEDICAL VIROLOGY}},
keywords = {{TRANSPLANTATION,INFECTION,PROTEIN,BLOOD MONONUCLEAR-CELLS,HEPATITIS-C-VIRUS,ANTIVIRAL THERAPY,OXIDATIVE STRESS,MINUS-STRAND,ACTIVATION,RNA}},
language = {{eng}},
number = {{9}},
pages = {{1569--1575}},
publisher = {{WILEY-LISS}},
title = {{Quantitation of Replication of the HCV Genome in Human Livers With End-Stage Cirrhosis by Strand-Specific Real-Time RT-PCR Assays: Methods and Clinical Relevance}},
url = {{http://dx.doi.org/10.1002/jmv.21510}},
volume = {{81}},
year = {{2009}},
}
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