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Integrated proteomic analysis reveals a substantial enrichment of protein trafficking processes in hippocampus tissue after hypoxic stress

Roos Van Elzen, Bart Ghesquière UGent, Evy Timmerman UGent, Stefaan Vandamme, Luc Moens, Kris Gevaert UGent and Sylvia Dewilde (2010) JOURNAL OF PROTEOME RESEARCH. 9(1). p.204-215
abstract
Acute and chronic hypoxic episodes of the brain have been generally recognized as a common denominator of several neuropathologies of which cerebral ischemic stroke represents one of the leading causes of mortality and morbidity. In an attempt to clarify the plethora of molecular events elicited by ischemic or hypoxic stress, several studies have reported before on large-scale expression analysis; however, only a minority have put focus on proteome based changes. To further enrich our knowledge, we investigated the differences in protein levels following prolonged exposure of mice to global hypoxic stress in the hippocampus, one of the most susceptible regions of the brain. This was accomplished using the conventional 2-DE approach and peptide-centered quantitative methionyl-COFRADIC. Together both methods resulted in the identification of 110 unique hypoxia regulated proteins, and 34 posthypoxic reoxygenation regulated proteins based on 2-DE analysis alone, The integration and comparison of the implicated biological functions with other large-scale analyses of similar hypoxia and ischemic stroke models gave an overall resemblance of implicated biological processes apart from model specific alterations in distribution. Nevertheless, further examination of the data clearly depicted a substantial enrichment of protein trafficking and targeting processes in our data which could be related to synaptic plasticity and remodeling events.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
RAT-BRAIN, BRAIN GENOMIC RESPONSE, IN-VITRO, peptide-centered, COFRADIC, two-dimensional electrophoresis, proteomics, hypoxia, Brain, FOCAL CEREBRAL-ISCHEMIA, MAGUK SCAFFOLDING PROTEINS, GENE-EXPRESSION, MICROARRAY ANALYSIS, MOLECULAR MECHANISMS, NEURONAL INJURY, ARTERY OCCLUSION
journal title
JOURNAL OF PROTEOME RESEARCH
J. Proteome Res.
volume
9
issue
1
pages
204 - 215
Web of Science type
Article
Web of Science id
000273267900020
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
5.46 (2010)
JCR rank
9/70 (2010)
JCR quartile
1 (2010)
ISSN
1535-3893
DOI
10.1021/pr900517m
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
902740
handle
http://hdl.handle.net/1854/LU-902740
date created
2010-03-12 13:53:06
date last changed
2012-11-28 15:42:15
@article{902740,
  abstract     = {Acute and chronic hypoxic episodes of the brain have been generally recognized as a common denominator of several neuropathologies of which cerebral ischemic stroke represents one of the leading causes of mortality and morbidity. In an attempt to clarify the plethora of molecular events elicited by ischemic or hypoxic stress, several studies have reported before on large-scale expression analysis; however, only a minority have put focus on proteome based changes. To further enrich our knowledge, we investigated the differences in protein levels following prolonged exposure of mice to global hypoxic stress in the hippocampus, one of the most susceptible regions of the brain. This was accomplished using the conventional 2-DE approach and peptide-centered quantitative methionyl-COFRADIC. Together both methods resulted in the identification of 110 unique hypoxia regulated proteins, and 34 posthypoxic reoxygenation regulated proteins based on 2-DE analysis alone, The integration and comparison of the implicated biological functions with other large-scale analyses of similar hypoxia and ischemic stroke models gave an overall resemblance of implicated biological processes apart from model specific alterations in distribution. Nevertheless, further examination of the data clearly depicted a substantial enrichment of protein trafficking and targeting processes in our data which could be related to synaptic plasticity and remodeling events.},
  author       = {Van Elzen, Roos and Ghesqui{\`e}re, Bart and Timmerman, Evy and Vandamme, Stefaan and Moens, Luc and Gevaert, Kris and Dewilde, Sylvia},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {RAT-BRAIN,BRAIN GENOMIC RESPONSE,IN-VITRO,peptide-centered,COFRADIC,two-dimensional electrophoresis,proteomics,hypoxia,Brain,FOCAL CEREBRAL-ISCHEMIA,MAGUK SCAFFOLDING PROTEINS,GENE-EXPRESSION,MICROARRAY ANALYSIS,MOLECULAR MECHANISMS,NEURONAL INJURY,ARTERY OCCLUSION},
  language     = {eng},
  number       = {1},
  pages        = {204--215},
  title        = {Integrated proteomic analysis reveals a substantial enrichment of protein trafficking processes in hippocampus tissue after hypoxic stress},
  url          = {http://dx.doi.org/10.1021/pr900517m},
  volume       = {9},
  year         = {2010},
}

Chicago
Van Elzen, Roos, Bart Ghesquière, Evy Timmerman, Stefaan Vandamme, Luc Moens, Kris Gevaert, and Sylvia Dewilde. 2010. “Integrated Proteomic Analysis Reveals a Substantial Enrichment of Protein Trafficking Processes in Hippocampus Tissue After Hypoxic Stress.” Journal of Proteome Research 9 (1): 204–215.
APA
Van Elzen, R., Ghesquière, B., Timmerman, E., Vandamme, S., Moens, L., Gevaert, K., & Dewilde, S. (2010). Integrated proteomic analysis reveals a substantial enrichment of protein trafficking processes in hippocampus tissue after hypoxic stress. JOURNAL OF PROTEOME RESEARCH, 9(1), 204–215.
Vancouver
1.
Van Elzen R, Ghesquière B, Timmerman E, Vandamme S, Moens L, Gevaert K, et al. Integrated proteomic analysis reveals a substantial enrichment of protein trafficking processes in hippocampus tissue after hypoxic stress. JOURNAL OF PROTEOME RESEARCH. 2010;9(1):204–15.
MLA
Van Elzen, Roos, Bart Ghesquière, Evy Timmerman, et al. “Integrated Proteomic Analysis Reveals a Substantial Enrichment of Protein Trafficking Processes in Hippocampus Tissue After Hypoxic Stress.” JOURNAL OF PROTEOME RESEARCH 9.1 (2010): 204–215. Print.