Ghent University Academic Bibliography

Advanced

Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers

A. Mihalyi, O. Gevaert, C.M. Kyama, P. Simsa, Nathalie Pochet UGent, F De Smet, B De Moor, C Meuleman, J Billen, N Blanckaert, et al. (2010) HUMAN REPRODUCTION. 25(3). p.654-664
abstract
Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis. This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM). Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100% (specificity 84%) and minimal-mild endometriosis was detected with a sensitivity of 87% (specificity 71%) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94% (specificity 61%) during the secretory phase and with a sensitivity of 92% (specificity 63%) during the menstrual phase. Advanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
EXPRESSION, MARKERS, PERITONEAL, MILD ENDOMETRIOSIS, MENSTRUAL-CYCLE, CA-125 LEVELS, SERUM CA-125, PELVIC PAIN, INTERLEUKIN-6, WOMEN
journal title
HUMAN REPRODUCTION
Hum. Reprod.
volume
25
issue
3
pages
11 pages
publisher
Oxford University Press
place of publication
OXFORD
Web of Science type
Article
Web of Science id
000274490700014
JCR category
OBSTETRICS & GYNECOLOGY
JCR impact factor
4.357 (2010)
JCR rank
3/75 (2010)
JCR quartile
1 (2010)
ISSN
0268-1161
DOI
10.1093/humrep/dep425
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
899595
handle
http://hdl.handle.net/1854/LU-899595
date created
2010-03-09 18:51:55
date last changed
2017-01-02 09:55:44
@article{899595,
  abstract     = {Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis.

This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM).

Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100\% (specificity 84\%) and minimal-mild endometriosis was detected with a sensitivity of 87\% (specificity 71\%) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94\% (specificity 61\%) during the secretory phase and with a sensitivity of 92\% (specificity 63\%) during the menstrual phase.

Advanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity.},
  author       = {Mihalyi, A. and Gevaert, O. and Kyama, C.M. and Simsa, P. and Pochet, Nathalie and De Smet, F and De Moor, B and Meuleman, C and Billen, J and Blanckaert, N and Vodolazkaia, A and Fulop, V and D'Hooghe, TM},
  issn         = {0268-1161},
  journal      = {HUMAN REPRODUCTION},
  keyword      = {EXPRESSION,MARKERS,PERITONEAL,MILD ENDOMETRIOSIS,MENSTRUAL-CYCLE,CA-125 LEVELS,SERUM CA-125,PELVIC PAIN,INTERLEUKIN-6,WOMEN},
  language     = {eng},
  number       = {3},
  pages        = {654--664},
  publisher    = {Oxford University Press},
  title        = {Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers},
  url          = {http://dx.doi.org/10.1093/humrep/dep425},
  volume       = {25},
  year         = {2010},
}

Chicago
Mihalyi, A., O. Gevaert, C.M. Kyama, P. Simsa, Nathalie Pochet, F De Smet, B De Moor, et al. 2010. “Non-invasive Diagnosis of Endometriosis Based on a Combined Analysis of Six Plasma Biomarkers.” Human Reproduction 25 (3): 654–664.
APA
Mihalyi, A., Gevaert, O., Kyama, C. M., Simsa, P., Pochet, N., De Smet, F., De Moor, B., et al. (2010). Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. HUMAN REPRODUCTION, 25(3), 654–664.
Vancouver
1.
Mihalyi A, Gevaert O, Kyama CM, Simsa P, Pochet N, De Smet F, et al. Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. HUMAN REPRODUCTION. OXFORD: Oxford University Press; 2010;25(3):654–64.
MLA
Mihalyi, A., O. Gevaert, C.M. Kyama, et al. “Non-invasive Diagnosis of Endometriosis Based on a Combined Analysis of Six Plasma Biomarkers.” HUMAN REPRODUCTION 25.3 (2010): 654–664. Print.