Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species
- Author
- Eva Neirinckx (UGent) , Chris Vervaet (UGent) , Joris Michiels (UGent) , Stefaan De Smet (UGent) , Wim Van Den Broeck (UGent) , Jean Paul Remon (UGent) , Patrick De Backer (UGent) and Siska Croubels (UGent)
- Organization
- Abstract
- The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.
- Keywords
- INTESTINAL PERMEABILITY, WATER-SOLUBLE DRUGS, RAT, BIOAVAILABILITY, ABSORPTION, REGIONS, HUMANS, TRACT, PHARMACOKINETICS, DYSFUNCTION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-899527
- MLA
- Neirinckx, Eva, et al. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, vol. 34, no. 3, 2011, pp. 290–97, doi:10.1111/j.1365-2885.2010.01218.x.
- APA
- Neirinckx, E., Vervaet, C., Michiels, J., De Smet, S., Van Den Broeck, W., Remon, J. P., … Croubels, S. (2011). Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 34(3), 290–297. https://doi.org/10.1111/j.1365-2885.2010.01218.x
- Chicago author-date
- Neirinckx, Eva, Chris Vervaet, Joris Michiels, Stefaan De Smet, Wim Van Den Broeck, Jean Paul Remon, Patrick De Backer, and Siska Croubels. 2011. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 34 (3): 290–97. https://doi.org/10.1111/j.1365-2885.2010.01218.x.
- Chicago author-date (all authors)
- Neirinckx, Eva, Chris Vervaet, Joris Michiels, Stefaan De Smet, Wim Van Den Broeck, Jean Paul Remon, Patrick De Backer, and Siska Croubels. 2011. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 34 (3): 290–297. doi:10.1111/j.1365-2885.2010.01218.x.
- Vancouver
- 1.Neirinckx E, Vervaet C, Michiels J, De Smet S, Van Den Broeck W, Remon JP, et al. Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS. 2011;34(3):290–7.
- IEEE
- [1]E. Neirinckx et al., “Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species,” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, vol. 34, no. 3, pp. 290–297, 2011.
@article{899527, abstract = {{The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.}}, author = {{Neirinckx, Eva and Vervaet, Chris and Michiels, Joris and De Smet, Stefaan and Van Den Broeck, Wim and Remon, Jean Paul and De Backer, Patrick and Croubels, Siska}}, issn = {{0140-7783}}, journal = {{JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS}}, keywords = {{INTESTINAL PERMEABILITY,WATER-SOLUBLE DRUGS,RAT,BIOAVAILABILITY,ABSORPTION,REGIONS,HUMANS,TRACT,PHARMACOKINETICS,DYSFUNCTION}}, language = {{eng}}, number = {{3}}, pages = {{290--297}}, title = {{Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species}}, url = {{http://doi.org/10.1111/j.1365-2885.2010.01218.x}}, volume = {{34}}, year = {{2011}}, }
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