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Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species

Eva Neirinckx UGent, Chris Vervaet UGent, Joris Michiels UGent, Stefaan De Smet UGent, Wim Van Den Broeck UGent, Jean Paul Remon UGent, Patrick De Backer UGent and Siska Croubels UGent (2011) JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS. 34(3). p.290-297
abstract
The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
INTESTINAL PERMEABILITY, WATER-SOLUBLE DRUGS, RAT, BIOAVAILABILITY, ABSORPTION, REGIONS, HUMANS, TRACT, PHARMACOKINETICS, DYSFUNCTION
journal title
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
J. Vet. Pharmacol. Ther.
volume
34
issue
3
pages
290 - 297
Web of Science type
Article
Web of Science id
000289627400011
JCR category
VETERINARY SCIENCES
JCR impact factor
1.181 (2011)
JCR rank
45/141 (2011)
JCR quartile
2 (2011)
ISSN
0140-7783
DOI
10.1111/j.1365-2885.2010.01218.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
899527
handle
http://hdl.handle.net/1854/LU-899527
date created
2010-03-09 16:52:15
date last changed
2011-12-12 08:57:19
@article{899527,
  abstract     = {The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.},
  author       = {Neirinckx, Eva and Vervaet, Chris and Michiels, Joris and De Smet, Stefaan and Van Den Broeck, Wim and Remon, Jean Paul and De Backer, Patrick and Croubels, Siska},
  issn         = {0140-7783},
  journal      = {JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS},
  keyword      = {INTESTINAL PERMEABILITY,WATER-SOLUBLE DRUGS,RAT,BIOAVAILABILITY,ABSORPTION,REGIONS,HUMANS,TRACT,PHARMACOKINETICS,DYSFUNCTION},
  language     = {eng},
  number       = {3},
  pages        = {290--297},
  title        = {Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species},
  url          = {http://dx.doi.org/10.1111/j.1365-2885.2010.01218.x},
  volume       = {34},
  year         = {2011},
}

Chicago
Neirinckx, Eva, Chris Vervaet, Joris Michiels, Stefaan De Smet, Wim Van Den Broeck, Jean Paul Remon, Patrick De Backer, and Siska Croubels. 2011. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” Journal of Veterinary Pharmacology and Therapeutics 34 (3): 290–297.
APA
Neirinckx, E., Vervaet, C., Michiels, J., De Smet, S., Van Den Broeck, W., Remon, J. P., De Backer, P., et al. (2011). Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 34(3), 290–297.
Vancouver
1.
Neirinckx E, Vervaet C, Michiels J, De Smet S, Van Den Broeck W, Remon JP, et al. Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS. 2011;34(3):290–7.
MLA
Neirinckx, Eva, Chris Vervaet, Joris Michiels, et al. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 34.3 (2011): 290–297. Print.