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Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species

Eva Neirinckx (UGent) , Chris Vervaet (UGent) , Joris Michiels (UGent) , Stefaan De Smet (UGent) , Wim Van Den Broeck (UGent) , Jean Paul Remon (UGent) , Patrick De Backer (UGent) and Siska Croubels (UGent)
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Abstract
The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.
Keywords
INTESTINAL PERMEABILITY, WATER-SOLUBLE DRUGS, RAT, BIOAVAILABILITY, ABSORPTION, REGIONS, HUMANS, TRACT, PHARMACOKINETICS, DYSFUNCTION

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Chicago
Neirinckx, Eva, Chris Vervaet, Joris Michiels, Stefaan De Smet, Wim Van Den Broeck, Jean Paul Remon, Patrick De Backer, and Siska Croubels. 2011. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” Journal of Veterinary Pharmacology and Therapeutics 34 (3): 290–297.
APA
Neirinckx, E., Vervaet, C., Michiels, J., De Smet, S., Van Den Broeck, W., Remon, J. P., De Backer, P., et al. (2011). Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 34(3), 290–297.
Vancouver
1.
Neirinckx E, Vervaet C, Michiels J, De Smet S, Van Den Broeck W, Remon JP, et al. Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species. JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS. 2011;34(3):290–7.
MLA
Neirinckx, Eva, Chris Vervaet, Joris Michiels, et al. “Feasibility of the Ussing Chamber Technique for the Determination of in Vitro Jejunal Permeability of Passively Absorbed Compounds in Different Animal Species.” JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS 34.3 (2011): 290–297. Print.
@article{899527,
  abstract     = {The aim of this study was to assess the feasibility of the Ussing chamber technique for the determination of the jejunal permeability of passively absorbed, high permeability model compounds (acetaminophen and ketoprofen) in different animal species. Additionally, electrophysiological measurements and histological examination of pre- and post-incubation tissue specimens were performed. Apparent permeability coefficients of turkey and dog jejunum were low and highly variable due to tissue fragility caused by differences in thickness of the remaining intestinal layers after stripping and resulting in severe damage. Pig and horse jejunum were markedly more suitable for permeability determinations and mild signs of deterioration were noticed after 120 min of incubation. Transepithelial electrical resistance and potential difference did not correlate well with the observed tissue damage. From these data, the Ussing chamber technique appears to allow for permeability measurements within a species, but seems unsuitable for interspecies permeability comparison. However, further validation of the method with low permeability compounds and actively transported compounds is needed.},
  author       = {Neirinckx, Eva and Vervaet, Chris and Michiels, Joris and De Smet, Stefaan and Van Den Broeck, Wim and Remon, Jean Paul and De Backer, Patrick and Croubels, Siska},
  issn         = {0140-7783},
  journal      = {JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS},
  keyword      = {INTESTINAL PERMEABILITY,WATER-SOLUBLE DRUGS,RAT,BIOAVAILABILITY,ABSORPTION,REGIONS,HUMANS,TRACT,PHARMACOKINETICS,DYSFUNCTION},
  language     = {eng},
  number       = {3},
  pages        = {290--297},
  title        = {Feasibility of the Ussing chamber technique for the determination of in vitro jejunal permeability of passively absorbed compounds in different animal species},
  url          = {http://dx.doi.org/10.1111/j.1365-2885.2010.01218.x},
  volume       = {34},
  year         = {2011},
}

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