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Dual detector micro-XRF cryotomography and mapping on the model organism Daphnia magna

Björn De Samber (UGent) , Gerd Wellenreuther, Roel Evens (UGent) , Karel De Schamphelaere (UGent) , Colin Janssen (UGent) , Inge Lindemann (UGent) , Geert Silversmit (UGent) , Tom Schoonjans (UGent) , Bart Vekemans (UGent) , Imre Szaloki, et al.
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Abstract
The recent availability of a cryostream cooler at beamline L has allowed synchrotron radiation based micro-XRF analysis of frozen biological samples close to their native state. In a previous contribution, we compared the elemental distributions within a ydrated (frozen) and a fixed (dehydrated) Daphnia magna, which is a freshwater crustacean used in toxicological research as a model organism for evaluating effects of metals on the cosystem. Although hydrated samples show less dislocation of elements and/or sample contamination as compared to fixed samples, they are mainly composed of a water matrix, which is more susceptible to absorption effects of low energy X-rays. Therefore, we investigate the degree of absorption in 2D/CT micro-XRF elemental maps of Daphnia magna using a dual silicon drift detector (SDD) setup.
Keywords
Daphnia magna, silicon drift detector, cryogenic, micro-XRF, synchrotron

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Chicago
De Samber, Björn, Gerd Wellenreuther, Roel Evens, Karel De Schamphelaere, Colin Janssen, Inge Lindemann, Geert Silversmit, et al. 2010. “Dual Detector micro-XRF Cryotomography and Mapping on the Model Organism Daphnia Magna.” Photon Science 2009 : Highlights and HASYLAB Annual Report : Author Contributions.
APA
De Samber, B., Wellenreuther, G., Evens, R., De Schamphelaere, K., Janssen, C., Lindemann, I., Silversmit, G., et al. (2010). Dual detector micro-XRF cryotomography and mapping on the model organism Daphnia magna. Photon Science 2009 : highlights and HASYLAB annual report : author contributions.
Vancouver
1.
De Samber B, Wellenreuther G, Evens R, De Schamphelaere K, Janssen C, Lindemann I, et al. Dual detector micro-XRF cryotomography and mapping on the model organism Daphnia magna. Photon Science 2009 : highlights and HASYLAB annual report : author contributions. Hamburg, Germany: Deutsches Elektronen-Synchrotron (DESY). Hamburger Synchrotronstrahlungslabor (HASYLAB); 2010;
MLA
De Samber, Björn, Gerd Wellenreuther, Roel Evens, et al. “Dual Detector micro-XRF Cryotomography and Mapping on the Model Organism Daphnia Magna.” Photon Science 2009 : highlights and HASYLAB annual report : author contributions (2010): n. pag. Print.
@article{898795,
  abstract     = {The recent availability of a cryostream cooler at beamline L has allowed synchrotron radiation based micro-XRF analysis of frozen biological samples close to their native state. In a previous contribution, we compared the elemental distributions within a  ydrated (frozen) and a fixed (dehydrated) Daphnia magna, which is a freshwater crustacean used in toxicological research  as a model organism for evaluating effects of metals on the  cosystem. Although hydrated samples show less dislocation of  elements and/or sample contamination as compared to fixed samples, they are mainly composed of a water matrix, which is more susceptible to absorption effects of low energy X-rays. Therefore, we investigate the degree of absorption in 2D/CT micro-XRF elemental maps of Daphnia magna using a dual silicon drift detector (SDD) setup.},
  articleno    = {2009480},
  author       = {De Samber, Bj{\"o}rn and Wellenreuther, Gerd and Evens, Roel and De Schamphelaere, Karel and Janssen, Colin and Lindemann, Inge and Silversmit, Geert and Schoonjans, Tom and Vekemans, Bart and Szaloki, Imre and Falkenberg, Gerald and Appel, Karen and Vincze, Laszlo},
  isbn         = {9783935702393},
  journal      = {Photon Science 2009 : highlights and HASYLAB annual report : author contributions},
  keyword      = {Daphnia magna,silicon drift detector,cryogenic,micro-XRF,synchrotron},
  language     = {eng},
  pages        = {2},
  publisher    = {Deutsches Elektronen-Synchrotron (DESY). Hamburger Synchrotronstrahlungslabor (HASYLAB)},
  title        = {Dual detector micro-XRF cryotomography and mapping on the model organism Daphnia magna},
  url          = {http://hasylab.desy.de/annual\_report/files/2009/2009480.pdf},
  year         = {2010},
}