Thermoresponsive poly(N-vinylcaprolactam) as stationary phase for aqueous and green liquid chromatography
- Author
- Bram Miserez (UGent) , Frederic Lynen (UGent) , Adrian Wright, Melvin Euerby and Patrick Sandra (UGent)
- Organization
- Abstract
- Poly(N-vinylcaprolactam) (PVCL) connected to aminopropyl silica is a new stationary phase for temperature responsive liquid chromatography (TR-LC). PVCL shows a transition from hydrophilic to hydrophobic interaction between 30 and 40 degrees C. The synthesis is described in detail. The temperature responsive characteristic of the phase is illustrated with a mixture of steroids using pure water as mobile phase. An increase in retention is observed when raising the temperature. H-u plots at different temperatures were constructed. Below the lower critical solution temperature (LCST), no optimal velocity could be measured because of substantial resistance to mass transfer. Above the LCST, u (opt) was ca. 0.3 mm s(-1) with reduced plate heights from 4 at 45 degrees C to 3 at 65 degrees C. The temperature responsive nature of the polymer is lost in green chromatography with ethanol as modifier in concentrations above 5%.
- Keywords
- N-ISOPROPYLACRYLAMIDE, POLYMER, MODIFIED SILICA, TEMPERATURE-RESPONSIVE CHROMATOGRAPHY, Poly(N-vinylcaprolactam), Aqueous and green mobile phases, Column liquid chromatography, Thermoresponsive liquid chromatography, SYSTEM, PH, VINYLCAPROLACTAM, COPOLYMERS, ONLINE, CELLS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-888915
- MLA
- Miserez, Bram, et al. “Thermoresponsive Poly(N-Vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography.” CHROMATOGRAPHIA, vol. 71, no. 1–2, 2010, pp. 1–6, doi:10.1365/s10337-009-1394-3.
- APA
- Miserez, B., Lynen, F., Wright, A., Euerby, M., & Sandra, P. (2010). Thermoresponsive poly(N-vinylcaprolactam) as stationary phase for aqueous and green liquid chromatography. CHROMATOGRAPHIA, 71(1–2), 1–6. https://doi.org/10.1365/s10337-009-1394-3
- Chicago author-date
- Miserez, Bram, Frederic Lynen, Adrian Wright, Melvin Euerby, and Patrick Sandra. 2010. “Thermoresponsive Poly(N-Vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography.” CHROMATOGRAPHIA 71 (1–2): 1–6. https://doi.org/10.1365/s10337-009-1394-3.
- Chicago author-date (all authors)
- Miserez, Bram, Frederic Lynen, Adrian Wright, Melvin Euerby, and Patrick Sandra. 2010. “Thermoresponsive Poly(N-Vinylcaprolactam) as Stationary Phase for Aqueous and Green Liquid Chromatography.” CHROMATOGRAPHIA 71 (1–2): 1–6. doi:10.1365/s10337-009-1394-3.
- Vancouver
- 1.Miserez B, Lynen F, Wright A, Euerby M, Sandra P. Thermoresponsive poly(N-vinylcaprolactam) as stationary phase for aqueous and green liquid chromatography. CHROMATOGRAPHIA. 2010;71(1–2):1–6.
- IEEE
- [1]B. Miserez, F. Lynen, A. Wright, M. Euerby, and P. Sandra, “Thermoresponsive poly(N-vinylcaprolactam) as stationary phase for aqueous and green liquid chromatography,” CHROMATOGRAPHIA, vol. 71, no. 1–2, pp. 1–6, 2010.
@article{888915,
abstract = {{Poly(N-vinylcaprolactam) (PVCL) connected to aminopropyl silica is a new stationary phase for temperature responsive liquid chromatography (TR-LC). PVCL shows a transition from hydrophilic to hydrophobic interaction between 30 and 40 degrees C. The synthesis is described in detail. The temperature responsive characteristic of the phase is illustrated with a mixture of steroids using pure water as mobile phase. An increase in retention is observed when raising the temperature. H-u plots at different temperatures were constructed. Below the lower critical solution temperature (LCST), no optimal velocity could be measured because of substantial resistance to mass transfer. Above the LCST, u (opt) was ca. 0.3 mm s(-1) with reduced plate heights from 4 at 45 degrees C to 3 at 65 degrees C. The temperature responsive nature of the polymer is lost in green chromatography with ethanol as modifier in concentrations above 5%.}},
author = {{Miserez, Bram and Lynen, Frederic and Wright, Adrian and Euerby, Melvin and Sandra, Patrick}},
issn = {{0009-5893}},
journal = {{CHROMATOGRAPHIA}},
keywords = {{N-ISOPROPYLACRYLAMIDE,POLYMER,MODIFIED SILICA,TEMPERATURE-RESPONSIVE CHROMATOGRAPHY,Poly(N-vinylcaprolactam),Aqueous and green mobile phases,Column liquid chromatography,Thermoresponsive liquid chromatography,SYSTEM,PH,VINYLCAPROLACTAM,COPOLYMERS,ONLINE,CELLS}},
language = {{eng}},
number = {{1-2}},
pages = {{1--6}},
title = {{Thermoresponsive poly(N-vinylcaprolactam) as stationary phase for aqueous and green liquid chromatography}},
url = {{http://doi.org/10.1365/s10337-009-1394-3}},
volume = {{71}},
year = {{2010}},
}
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