Advanced search
1 file | 278.49 KB

Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation

(2010) ALLERGY. 65(8). p.1013-1020
Author
Organization
Abstract
Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages. Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells. Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied. Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.
Keywords
IGE-ANTIBODIES, BRONCHIAL EOSINOPHILIA, TOXIC-SHOCK-SYNDROME, superantigen, chemokine, epithelial cell, Staphylococcus aureus enterotoxin B, NASAL, INFLAMMATION, STIMULATION, ALLERGIC RHINITIS, ATOPIC-DERMATITIS, DENDRITIC CELLS, BACTERIAL SUPERANTIGEN

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 278.49 KB

Citation

Please use this url to cite or link to this publication:

Chicago
HUVENNE, WOUTER, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” Allergy 65 (8): 1013–1020.
APA
HUVENNE, W., Callebaut, I., Reekmans, K., Hens, G., Bobic, S., Jorissen, M., Bullens, D., et al. (2010). Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY, 65(8), 1013–1020.
Vancouver
1.
HUVENNE W, Callebaut I, Reekmans K, Hens G, Bobic S, Jorissen M, et al. Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY. 2010;65(8):1013–20.
MLA
HUVENNE, WOUTER, Ina Callebaut, Kristien Reekmans, et al. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65.8 (2010): 1013–1020. Print.
@article{886432,
  abstract     = {Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages.
Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells.
Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied.
Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.},
  author       = {Huvenne, Wouter and Callebaut, Ina and Reekmans, Kristien and Hens, Greet and Bobic, Sonja and Jorissen, Mark and Bullens, Dominique and Ceuppens, Jan and Bachert, Claus and Hellings, Peter},
  issn         = {0105-4538},
  journal      = {ALLERGY},
  language     = {eng},
  number       = {8},
  pages        = {1013--1020},
  title        = {Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2009.02313.x},
  volume       = {65},
  year         = {2010},
}

Altmetric
View in Altmetric
Web of Science
Times cited: