
Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation
- Author
- Wouter Huvenne (UGent) , Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert (UGent) and Peter Hellings
- Organization
- Abstract
- Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages. Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells. Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied. Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.
- Keywords
- IGE-ANTIBODIES, BRONCHIAL EOSINOPHILIA, TOXIC-SHOCK-SYNDROME, superantigen, chemokine, epithelial cell, Staphylococcus aureus enterotoxin B, NASAL, INFLAMMATION, STIMULATION, ALLERGIC RHINITIS, ATOPIC-DERMATITIS, DENDRITIC CELLS, BACTERIAL SUPERANTIGEN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-886432
- MLA
- Huvenne, Wouter, et al. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY, vol. 65, no. 8, 2010, pp. 1013–20, doi:10.1111/j.1398-9995.2009.02313.x.
- APA
- Huvenne, W., Callebaut, I., Reekmans, K., Hens, G., Bobic, S., Jorissen, M., … Hellings, P. (2010). Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY, 65(8), 1013–1020. https://doi.org/10.1111/j.1398-9995.2009.02313.x
- Chicago author-date
- Huvenne, Wouter, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65 (8): 1013–20. https://doi.org/10.1111/j.1398-9995.2009.02313.x.
- Chicago author-date (all authors)
- Huvenne, Wouter, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65 (8): 1013–1020. doi:10.1111/j.1398-9995.2009.02313.x.
- Vancouver
- 1.Huvenne W, Callebaut I, Reekmans K, Hens G, Bobic S, Jorissen M, et al. Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY. 2010;65(8):1013–20.
- IEEE
- [1]W. Huvenne et al., “Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation,” ALLERGY, vol. 65, no. 8, pp. 1013–1020, 2010.
@article{886432, abstract = {{Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages. Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells. Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied. Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.}}, author = {{Huvenne, Wouter and Callebaut, Ina and Reekmans, Kristien and Hens, Greet and Bobic, Sonja and Jorissen, Mark and Bullens, Dominique and Ceuppens, Jan and Bachert, Claus and Hellings, Peter}}, issn = {{0105-4538}}, journal = {{ALLERGY}}, keywords = {{IGE-ANTIBODIES,BRONCHIAL EOSINOPHILIA,TOXIC-SHOCK-SYNDROME,superantigen,chemokine,epithelial cell,Staphylococcus aureus enterotoxin B,NASAL,INFLAMMATION,STIMULATION,ALLERGIC RHINITIS,ATOPIC-DERMATITIS,DENDRITIC CELLS,BACTERIAL SUPERANTIGEN}}, language = {{eng}}, number = {{8}}, pages = {{1013--1020}}, title = {{Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation}}, url = {{http://doi.org/10.1111/j.1398-9995.2009.02313.x}}, volume = {{65}}, year = {{2010}}, }
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