Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation
- Author
- Wouter Huvenne (UGent) , Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert (UGent) and Peter Hellings
- Organization
- Abstract
- Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages. Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells. Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied. Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.
- Keywords
- IGE-ANTIBODIES, BRONCHIAL EOSINOPHILIA, TOXIC-SHOCK-SYNDROME, superantigen, chemokine, epithelial cell, Staphylococcus aureus enterotoxin B, NASAL, INFLAMMATION, STIMULATION, ALLERGIC RHINITIS, ATOPIC-DERMATITIS, DENDRITIC CELLS, BACTERIAL SUPERANTIGEN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-886432
- Chicago
- HUVENNE, WOUTER, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” Allergy 65 (8): 1013–1020.
- APA
- HUVENNE, W., Callebaut, I., Reekmans, K., Hens, G., Bobic, S., Jorissen, M., Bullens, D., et al. (2010). Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY, 65(8), 1013–1020.
- Vancouver
- 1.HUVENNE W, Callebaut I, Reekmans K, Hens G, Bobic S, Jorissen M, et al. Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY. 2010;65(8):1013–20.
- MLA
- HUVENNE, WOUTER, Ina Callebaut, Kristien Reekmans, et al. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65.8 (2010): 1013–1020. Print.
@article{886432,
abstract = {Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages.
Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells.
Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied.
Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.},
author = {Huvenne, Wouter and Callebaut, Ina and Reekmans, Kristien and Hens, Greet and Bobic, Sonja and Jorissen, Mark and Bullens, Dominique and Ceuppens, Jan and Bachert, Claus and Hellings, Peter},
issn = {0105-4538},
journal = {ALLERGY},
language = {eng},
number = {8},
pages = {1013--1020},
title = {Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation},
url = {http://dx.doi.org/10.1111/j.1398-9995.2009.02313.x},
volume = {65},
year = {2010},
}
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