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Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation

(2010) ALLERGY. 65(8). p.1013-1020
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Abstract
Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages. Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells. Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied. Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.
Keywords
IGE-ANTIBODIES, BRONCHIAL EOSINOPHILIA, TOXIC-SHOCK-SYNDROME, superantigen, chemokine, epithelial cell, Staphylococcus aureus enterotoxin B, NASAL, INFLAMMATION, STIMULATION, ALLERGIC RHINITIS, ATOPIC-DERMATITIS, DENDRITIC CELLS, BACTERIAL SUPERANTIGEN

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MLA
Huvenne, Wouter, et al. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY, vol. 65, no. 8, 2010, pp. 1013–20, doi:10.1111/j.1398-9995.2009.02313.x.
APA
Huvenne, W., Callebaut, I., Reekmans, K., Hens, G., Bobic, S., Jorissen, M., … Hellings, P. (2010). Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY, 65(8), 1013–1020. https://doi.org/10.1111/j.1398-9995.2009.02313.x
Chicago author-date
Huvenne, Wouter, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65 (8): 1013–20. https://doi.org/10.1111/j.1398-9995.2009.02313.x.
Chicago author-date (all authors)
Huvenne, Wouter, Ina Callebaut, Kristien Reekmans, Greet Hens, Sonja Bobic, Mark Jorissen, Dominique Bullens, Jan Ceuppens, Claus Bachert, and Peter Hellings. 2010. “Staphylococcus Aureus Enterotoxin B Augments Granulocyte Migration and Survival via Airway Epithelial Cell Activation.” ALLERGY 65 (8): 1013–1020. doi:10.1111/j.1398-9995.2009.02313.x.
Vancouver
1.
Huvenne W, Callebaut I, Reekmans K, Hens G, Bobic S, Jorissen M, et al. Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation. ALLERGY. 2010;65(8):1013–20.
IEEE
[1]
W. Huvenne et al., “Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation,” ALLERGY, vol. 65, no. 8, pp. 1013–1020, 2010.
@article{886432,
  abstract     = {{Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages.
Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells.
Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied.
Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.}},
  author       = {{Huvenne, Wouter and Callebaut, Ina and Reekmans, Kristien and Hens, Greet and Bobic, Sonja and Jorissen, Mark and Bullens, Dominique and Ceuppens, Jan and Bachert, Claus and Hellings, Peter}},
  issn         = {{0105-4538}},
  journal      = {{ALLERGY}},
  keywords     = {{IGE-ANTIBODIES,BRONCHIAL EOSINOPHILIA,TOXIC-SHOCK-SYNDROME,superantigen,chemokine,epithelial cell,Staphylococcus aureus enterotoxin B,NASAL,INFLAMMATION,STIMULATION,ALLERGIC RHINITIS,ATOPIC-DERMATITIS,DENDRITIC CELLS,BACTERIAL SUPERANTIGEN}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1013--1020}},
  title        = {{Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation}},
  url          = {{http://doi.org/10.1111/j.1398-9995.2009.02313.x}},
  volume       = {{65}},
  year         = {{2010}},
}

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