@article{886432,
  abstract     = {{Background: Staphylococcus aureus enterotoxin B (SEB) has recently been postulated to be involved in the pathology of granulocyte-dominated disease. Studying the immunologic interaction between SEB and airway epithelial cells in immortalized cell lines or long term epithelial cell cultures has obvious disadvantages.
Methods: We used a novel technique of freshly isolated and purified human nasal epithelial cells from healthy, non-allergic individuals, which were incubated for 24 h without/with SEB at different concentrations. Chemokine production was evaluated in the supernatant using Cytometric Bead Array. The chemotactic activity of the supernatant was studied in vitro using a Boyden chamber. Survival was evaluated with flow cytometry, using propidium iodide to identify dead cells.
Results: SEB showed a dose-dependent induction of IP-10, MIG, RANTES, MCP-1 and G-CSF production by epithelial cells in vitro. The supernatant of epithelial cells had chemotactic activity for granulocytes in vitro, which was enhanced in the supernatant of SEB-stimulated epithelial cells. Reduced number of propidium iodide positive granulocytes was found in the conditions where supernatant of SEB-stimulated epithelial cells was applied.
Conclusion: Staphylococcus aureus enterotoxin B exerts a direct pro-inflammatory effect on human nasal epithelial cells, with induction of chemokine and growth factor release, resulting in the migration and prolonged survival of granulocytes in vitro.}},
  author       = {{Huvenne, Wouter and Callebaut, Ina and Reekmans, Kristien and Hens, Greet and Bobic, Sonja and Jorissen, Mark and Bullens, Dominique and Ceuppens, Jan and Bachert, Claus and Hellings, Peter}},
  issn         = {{0105-4538}},
  journal      = {{ALLERGY}},
  keywords     = {{IGE-ANTIBODIES,BRONCHIAL EOSINOPHILIA,TOXIC-SHOCK-SYNDROME,superantigen,chemokine,epithelial cell,Staphylococcus aureus enterotoxin B,NASAL,INFLAMMATION,STIMULATION,ALLERGIC RHINITIS,ATOPIC-DERMATITIS,DENDRITIC CELLS,BACTERIAL SUPERANTIGEN}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1013--1020}},
  title        = {{Staphylococcus aureus enterotoxin B augments granulocyte migration and survival via airway epithelial cell activation}},
  url          = {{http://doi.org/10.1111/j.1398-9995.2009.02313.x}},
  volume       = {{65}},
  year         = {{2010}},
}

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